Chemical structure and biological activity of the (1 → 3)-linked β-D-glucan isolated from marine diatom Conticribra weissflogii

Rizzi, Juliane; Moro, Tatiana Rojo; Winnischofer, Sheila Maria Brochado; Colusse, Guilherme Augusto; Tamiello, Camila Silva; Trombetta-Lima, Marina; Noleto, Guilhermina Rodrigues; Dolga, Amalia M.; Duarte, Maria Eugênia R.; Noseda, Miguel D.

doi:10.1016/j.ijbiomac.2022.10.147

Cited by 4 publications

(3 citation statements)

References 57 publications

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“…To the best of our knowledge, this is the first study that characterizes metabolites through assays with 1 H HR-MAS NMR of C. weissflogii , which is a model species for environmental studies and in the development of energy and feed supplements for animals [ 47 , 48 ]. Since before its reclassification (previously known as Thalassiosira weissflogii ) [ 48 , 49 ], this species has demonstrated important cytotoxic and immunomodulatory bioactivity related to the presence of polysaccharides, as well as the important production of metabolites that are toxic against different microorganisms; for example, it interferes with quorum sensing and the adhesion and formation of bacterial biofilms [ 47 , 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Application of 1H HR-MAS NMR-Based Metabolite Fingerprinting of Marine Microalgae

et al. 2023

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Natural products from the marine environment as well as microalgae, have been known for the complexity of the metabolites they produce due to their adaptability to different environmental conditions, which has been an inexhaustible source of several bioactive properties, such as antioxidant, anti-tumor, and antimicrobial. This study aims to characterize the main metabolites of three species of microalgae (Nannochloropsis oceanica, Chaetoceros muelleri, and Conticribra weissflogii), which have important applications in the biofuel and nutrition industries, by 1H High-resolution magic angle spinning nuclear magnetic resonance (1H HR-MAS NMR), a method which is non-destructive, is highly reproducible, and requires minimal sample preparation. Even though the three species were found in the same ecosystem and a superior production of lipid compounds was observed, important differences were identified in relation to the production of specialized metabolites. These distinct properties favor the use of these compounds as leaders in the development of new bioactive compounds, especially against environmental, human, and animal pathogens (One Health), and demonstrate their potential in the development of alternatives for aquaculture.

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Section: Discussionmentioning

confidence: 99%

Application of 1H HR-MAS NMR-Based Metabolite Fingerprinting of Marine Microalgae

et al. 2023

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“…An immunomodulatory β-glucan, identified from marine diatom Conticribra weissflogii, was investigated completely through 1D 1 H, 13 C, and 2D 1 H- 13 C NMR methodology. 45 The δ H1 /δ C1 values at 4.78/102.5 ppm established a β-anomeric configuration for the isolated glucan, while a down-field shift solely in 1 H3/ 13 C3 (δ H3 /δ C3 3.78/84.8 ppm) indicated towards a linear glucan structure 3-linked primarily composed of the !3)-β-Glc-(1 ! unit.…”

Section: Overview Of Nmr In Studies Of Marine Sulfated Glycans: Chall...mentioning

confidence: 93%

Nuclear magnetic resonance‐based structural elucidation of novel marine glycans and derived oligosaccharides

Dwivedi

Maurya

Ahmed

et al. 2023

Magnetic Reson in Chemistry

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Marine glycans of defined structures are unique representatives among all kinds of structurally complex glycans endowed with important biological actions. Besides their unique biological properties, these marine sugars also enable advanced structure–activity relationship (SAR) studies given their distinct and defined structures. However, the natural high molecular weights (MWs) of these marine polysaccharides, sometimes even bigger than 100 kDa, pose a problem in many biophysical and analytical studies. Hence, the preparation of low MW oligosaccharides becomes a strategy to overcome the problem. Regardless of the polymeric or oligomeric lengths of these molecules, structural elucidation is mandatory for SAR studies. For this, nuclear magnetic resonance (NMR) spectroscopy plays a pivotal role. Here, we revisit the NMR‐based structural elucidation of a series of marine sulfated poly/oligosaccharides discovered in our laboratory within the last 2 years. This set of structures includes the α‐glucan extracted from the bivalve Marcia hiantina; the two sulfated galactans extracted from the red alga Botryocladia occidentalis; the fucosylated chondroitin sulfate isolated from the sea cucumber Pentacta pygmaea; the oligosaccharides produced from the fucosylated chondroitin sulfates from this sea cucumber species and from another species, Holothuria floridana; and the sulfated fucan from this later species. Specific 1H and 13C chemical shifts, generated by various 1D and 2D homonuclear and heteronuclear NMR spectra, are exploited as the primary source of information in the structural elucidation of these marine glycans.

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“…In respect to the structure, cereal β-glucan is mainly linked by β-1,3 and β-1,4 glycosidic bonds, while microbial or algal β-glucan is mainly linked by β-1,3 and β-1,6 glycosidic bonds. In terms of biological activity, oat β-glucan can significantly reduce fasting blood sugar, improve glucose tolerance and insulin sensitivity, and achieve a hypoglycemic effect [ 17 ]; Ganoderma lucidum spores β-glucan and marine diatom β-glucan have immune-enhancing effects [ 18 , 19 ].In terms of application, Lentinula edodes β-glucan, yeast β-glucan and oat β-glucan have various effects on starch pasting, gelation, and digestive properties due to their distinct molecular weights and spatial conformations. [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Developments in Molecular Characterization, Bioactivity, and Application of Arabinoxylans from Different Sources

et al. 2023

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Arabinoxylan (AX) is a polysaccharide composed of arabinose, xylose, and a small number of other carbohydrates. AX comes from a wide range of sources, and its physicochemical properties and physiological functions are closely related to its molecular characterization, such as branched chains, relative molecular masses, and substituents. In addition, AX also has antioxidant, hypoglycemic, antitumor, and proliferative abilities for intestinal probiotic flora, among other biological activities. AXs of various origins have different molecular characterizations in terms of molecular weight, degree of branching, and structure, with varying structures leading to diverse effects of the biological activity of AX. Therefore, this report describes the physical properties, biological activities, and applications of AX in diverse plants, aiming to provide a theoretical basis for future research on AX as well as provide more options for crop breeding.

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Chemical structure and biological activity of the (1 → 3)-linked β-D-glucan isolated from marine diatom Conticribra weissflogii

Cited by 4 publications

References 57 publications

Application of 1H HR-MAS NMR-Based Metabolite Fingerprinting of Marine Microalgae

Application of 1H HR-MAS NMR-Based Metabolite Fingerprinting of Marine Microalgae

Nuclear magnetic resonance‐based structural elucidation of novel marine glycans and derived oligosaccharides

Recent Developments in Molecular Characterization, Bioactivity, and Application of Arabinoxylans from Different Sources

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