Chemical strategies for immobilization of oligonucleotides

Abstract

The development of oligonucleotide-based microarrays (biochips) is a major thrust area in the rapidly growing biotechnology industry, which encompasses a diverse range of research areas including genomics, proteomics, computational biology, and pharmaceuticals, among other activities. Microarray experiments have proved to be unique in offering cost-effective and efficient analysis at the genomic level. In the last few years, biochips have gained increasing acceptance in the study of genetic and cellular processes. As the increase in experimental throughput has posed many challenges to the research community, considerable progress has been made in the advancement of microarray technology. In this review, chemical strategies for immobilization of oligonucleotides have been highlighted with special emphasis on post-synthetic immobilization of oligonucleotides on glass surface. The major objective of this article is to make the researchers acquainted with some most recent advances in this area.

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

“…A variety of strategies have been reported for the immobilization of bioreceptors to sensor coatings [3,15,50,62,63,210]. Most of them can also be applied to the immobilization of b i o r e c e p t o r s t o a n t i f o u l i n g c o a t i n g s ( s e e a l s o "Immobilization of biorecognition elements" section).…”

Genotyping of single nucleotide polymorphisms related to attention-deficit hyperactivity disorder

“…The system is based on microarray assays, performed on polycarbonate chips, and on the later colorimetric detection by a biorecognition process. The use of polymeric substrates is a good solution in research [14], but is key for 65 commercial purposes. Polycarbonate is an excellent material given its properties: mass fabrication under high quality standards, adaptable fabrication of devices, exceptional optical 4 properties, wide chemical reactivity for probe anchoring [15,16].…”

Editorial: Biochips and nanobiotechnology

“…The first article reviews chemical strategies for immobilization of oligonucleotides with special emphasis on post-synthetic immobilization on glass surfaces [1]. After the advent of biochip technology, an enormous amount of reports has been published about the various related technologies and their applications.…”