Chemical stability of a cold-active cellulase with high tolerance toward surfactants and chaotropic agent

Souza, Thaís Vieira de; Araújo, Juscemácia N.; Silva, Viviam M. da; Liberato, M.V.; Pimentel, Agnes C.; Alvarez, Thabata M.; Squina, Fábio M.; Garcia, Wânius

doi:10.1016/j.btre.2015.11.001

Cited by 21 publications

(6 citation statements)

References 26 publications

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“…Cold-adapted cellulases can be used in the textile and paper industries, in cotton processing, paper recycling, detergent production, juice extraction, as animal feed additives, and for biofuel production [13,66,95,96]. Cold-adapted cellulolytic enzymes tolerant to organic solvents can be used for manufacturing volatile and heat-sensitive compounds such as flavors, fragrances, and perfumes [97].…”

Section: Cellulasesmentioning

confidence: 99%

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Duarte

Santos

Vianna

et al. 2017

Critical Reviews in Biotechnology

106

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Antarctica is the coldest, windiest, and driest continent on Earth. In this sense, microorganisms that inhabit Antarctica environments have to be adapted to harsh conditions. Fungal strains affiliated with Ascomycota and Basidiomycota phyla have been recovered from terrestrial and marine Antarctic samples. They have been used for the bioprospecting of molecules, such as enzymes. Many reports have shown that these microorganisms produce cold-adapted enzymes at low or mild temperatures, including hydrolases (e.g. α-amylase, cellulase, chitinase, glucosidase, invertase, lipase, pectinase, phytase, protease, subtilase, tannase, and xylanase) and oxidoreductases (laccase and superoxide dismutase). Most of these enzymes are extracellular and their production in the laboratory has been carried out mainly under submerged culture conditions. Several studies showed that the cold-adapted enzymes exhibit a wide range in optimal pH (1.0-9.0) and temperature (10.0-70.0 °C). A myriad of methods have been applied for cold-adapted enzyme purification, resulting in purification factors and yields ranging from 1.70 to 1568.00-fold and 0.60 to 86.20%, respectively. Additionally, some fungal cold-adapted enzymes have been cloned and expressed in host organisms. Considering the enzyme-producing ability of microorganisms and the properties of cold-adapted enzymes, fungi recovered from Antarctic environments could be a prolific genetic resource for biotechnological processes (industrial and environmental) carried out at low or mild temperatures.

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Section: Cellulasesmentioning

confidence: 99%

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Duarte

Santos

Vianna

et al. 2017

Critical Reviews in Biotechnology

106

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“…Moreover, CWDE-inhibiting proteins, which are widely distributed in the plant cell wall as a defence mechanism (Benedetti et al, 2018b;Juge 2006;Kalunke et al, 2015;Locci et al, 2019;York et al, 2004), are also inactivated at high temperatures (Liu et al, 2017;Locci et al, 2019), thus do not interfere with enzymatic cellulose degradation. Furthermore, the structural stability of HCWDEs sustains their activity even in the presence of chemicals, surfactants and extreme pH ( de Miguel Bouzas et al 2006;Souza et al 2016). Such harsh reaction conditions may be exploited in industrial applications to promote detachment of different lignocellulose components, thus further increasing the efficiency of HCWDE enzymatic hydrolysis (Li et al 2016;Ooshima et al 1986).…”

Section: Introductionmentioning

confidence: 99%

“…2006; Souza et al . 2016). Such harsh reaction conditions may be exploited in industrial applications to promote detachment of different lignocellulose components, thus further increasing the efficiency of HCWDE enzymatic hydrolysis (Li et al .…”

Section: Introductionmentioning

confidence: 99%

A microalgal‐based preparation with synergistic cellulolytic and detoxifying action towards chemical‐treated lignocellulose

Benedetti

Barera

Longoni

et al. 2020

Plant Biotechnology Journal

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High-temperature bioconversion of lignocellulose into fermentable sugars has drawn attention for efficient production of renewable chemicals and biofuels, because competing microbial activities are inhibited at elevated temperatures and thermostable cell wall degrading enzymes are superior to mesophilic enzymes. Here, we report on the development of a platform to produce four different thermostable cell wall degrading enzymes in the chloroplast of Chlamydomonas reinhardtii. The enzyme blend was composed of the cellobiohydrolase CBM3GH5 from C. saccharolyticus, the b-glucosidase celB from P. furiosus, the endoglucanase B and the endoxylanase XynA from T. neapolitana. In addition, transplastomic microalgae were engineered for the expression of phosphite dehydrogenase D from Pseudomonas stutzeri, allowing for growth in non-axenic media by selective phosphite nutrition. The cellulolytic blend composed of the glycoside hydrolase (GH) domain GH12/GH5/GH1 allowed the conversion of alkaline-treated lignocellulose into glucose with efficiencies ranging from 14% to 17% upon 48h of reaction and an enzyme loading of 0.05% (w/w). Hydrolysates from treated cellulosic materials with extracts of transgenic microalgae boosted both the biogas production by methanogenic bacteria and the mixotrophic growth of the oleaginous microalga Chlorella vulgaris. Notably, microalgal treatment suppressed the detrimental effect of inhibitory byproducts released from the alkaline treatment of biomass, thus allowing for efficient assimilation of lignocellulose-derived sugars by C. vulgaris under mixotrophic growth.

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“…Importantly, high temperatures inactivate plant CWLE-proteinaceous inhibitors, which are localized in the cell wall as a defense mechanism [38,124,125], thus preventing them from interfering with enzymatic degradation. Moreover, the robust protein structure, a common feature of many HCWLEs, allows their use even in the presence of anionic surfactants, extreme pH conditions, and harsh chemicals [126]. In turn, industrial practices can exploit these severe conditions in order to deconstruct lignocellulose, favoring downstream enzymatic reactions [127].…”

Section: Heterologous Expression Of Cwles In Plantsmentioning

confidence: 99%

Green Production and Biotechnological Applications of Cell Wall Lytic Enzymes

et al. 2019

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Energy demand is constantly growing, and, nowadays, fossil fuels still play a dominant role in global energy production, despite their negative effects on air pollution and the emission of greenhouse gases, which are the main contributors to global warming. An alternative clean source of energy is represented by the lignocellulose fraction of plant cell walls, the most abundant carbon source on Earth. To obtain biofuels, lignocellulose must be efficiently converted into fermentable sugars. In this regard, the exploitation of cell wall lytic enzymes (CWLEs) produced by lignocellulolytic fungi and bacteria may be considered as an eco-friendly alternative. These organisms evolved to produce a variety of highly specific CWLEs, even if in low amounts. For an industrial use, both the identification of novel CWLEs and the optimization of sustainable CWLE-expressing biofactories are crucial. In this review, we focus on recently reported advances in the heterologous expression of CWLEs from microbial and plant expression systems as well as some of their industrial applications, including the production of biofuels from agricultural feedstock and of value-added compounds from waste materials. Moreover, since heterologous expression of CWLEs may be toxic to plant hosts, genetic strategies aimed in converting such a deleterious effect into a beneficial trait are discussed.

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Chemical stability of a cold-active cellulase with high tolerance toward surfactants and chaotropic agent

Abstract: HighlightsSurfactants were not able to reduce CelE1 activity significantly.CelE1 was found to be promising candidate for use as detergent additives.Chemical unfolding of CelE1 was very nearly completely reversible.Chemical unfolding of CelE1 proceeds as a two-state process.

Cited by 21 publications

References 26 publications

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

A microalgal‐based preparation with synergistic cellulolytic and detoxifying action towards chemical‐treated lignocellulose

Green Production and Biotechnological Applications of Cell Wall Lytic Enzymes

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