Chemical speciation of MeHg⁺ and Hg²⁺ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

Abstract

Speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches with fluorogenic probes supported in cultured cells.

Detection of Contaminants of High Environmental Impact by Means of Fluorogenic Probes

“…The experiments were a good starting point for the preparation of selective MeHg 1 fluorogenic probes for live-cell imaging by synthesizing water-soluble derivatives of the probes that retained the sensitivity to Hg(II) derivatives, albeit one of them, JG45, was much more sensitive to MeHg , and the fluorogenic probes. [43] 5. Discriminating organic compounds of serious concern: From ecstasy to nerve agents…”

“…Following this idea, we prepared new fluorogenic probes for the selective detection and speciation of Hg 21 and MeHg 1 in aqueousorganic mixtures and in human embryonic kidney cell line 293 (HEK293) cells. [43] Both probes were very efficient off-on fluorogenic probes for the selective detection of , in all tested solvent mixtures, whereas the other one produced a fluorescent response only in the presence of Hg 21 . As a consequence, the use of both probes permitted the speciation of MeHg 1 and Hg 21 in aqueous test samples containing mixtures of both cations, performed for the first time only with the help of fluorogenic probes using the standard addition methodology (Figure 10).…”

“…Subsequent interaction of the sulfur‐containing group with thiophilic cations should therefore increase the fluorescence of the probe, thus making this kind of compound suitable for the detection of Hg(II) cations. Following this idea, we prepared new fluorogenic probes for the selective detection and speciation of Hg 2+ and MeHg + in aqueous‐organic mixtures and in human embryonic kidney cell line 293 (HEK293) cells . Both probes were very efficient off‐on fluorogenic probes for the selective detection of Hg 2+ in mixtures of methanol: water 20: 80 V/V or acetonitrile: water 20: 80 V/V with comparable efficiency.…”

Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus

“…Indeed, the rare examples only include nuclearlocalized probes NucPE1, SNAP-PG and a highly sensitive probe NP1 responding to the changes of nuclear H 2 O 2 uxes, [33][34][35] a photo-stable pH probe NaOCy and a ratiometric uorescence pH probe hoeFL for selectively staining nuclei in living cells; 36,37 a sulfur-containing uorogenic probe JG45 for selectively detecting nuclear methylmercury (MeHg 2+ ). 38 The difficulty in visualizing the ux of bioactive molecules in the nucleus is mainly due to the impermeable nuclear membrane and the interference of the complex nucleic acid environment with the uorescence of probes. 39,40 On the other hand, numerous DNA and RNA binding agents have been reported, exhibiting nucleic acid induced uorescence turn-on properties for selectively staining the DNA-rich nucleus and RNA-rich nucleolus.…”

A Highly Selective and Ultrasensitive Fluorescent Probe for Monitoring Hg2+ and Its Applications in Real Water Samples

“…[21][22][23][24][25] There are many fluorescent probes to determinate Hg 2+ , but lots of the reported fluorescent probes are based upon foldamers, polymers, and biomolecules. [26][27][28][29][30][31] In addition, most of these reported probes detect Hg 2+ with difficulty in the aqueous solution, and have low sensitive in actual operations, which make the detection become harder, and even failure. [32][33][34][35][36][37] Therefore, it is vital to research new fluorescent probes with water-solubility, high sensitivity and selectivity to detect mercury ions.…”