Chemical screening: A simple approach to visualizing Streptomyces diversity for drug discovery and further research

Taddei, Antonieta; Valderrama, Margaret; Giarrizzo, Juan G.; Rey, Maikahl; Castelli, Cristina

doi:10.1016/j.resmic.2005.07.007

Cited by 14 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This means that each isolate has its own spectrum of antimicrobial activity, again demonstrating the physiological and morphological diversity of these isolates. Taddei et al (2006) evaluated the diversity of metabolites produced by 71 Streptomyces isolates, and found only two isolates that showed the same pattern.…”

Section: Discussionmentioning

confidence: 99%

Preliminary characterization of some Streptomyces species isolated from a composting process and their antimicrobial potential

Salamoni

Mann

Campos

et al. 2010

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The aim of this study was to screen Streptomycetes isolates with antimicrobial and antiviral activity, in a search for new metabolites. The isolates were obtained from a composting process, and identified based on morphological characteristics and molecular biological methods. The antimicrobial activity was determined using the double-layer agar method against 53 test organisms (bacteria, yeasts, and filamentous fungi). All isolates were grown in submerged culture, in mineral salts-starch-casein (SC) broth and ISP2 media, and the filtrate cultures were used in the assays for antibacterial and antiviral activity. Bovine Herpes virus (BoHV-I) was used for the antiviral activity. The morphological and molecular characteristics confirmed that all 25 isolates belonged to the genus Streptomyces. In the assay for antimicrobial activity, 80% of the Streptomyces isolates were able to inhibit at least one of the test organisms. Of these, 80% were active against bacteria and 45% against fungi. Eight of the isolates showed a broad spectrum of inhibitory activity; of these, the isolate Streptomyces spp. 1S was able to inhibit 46 of the test organisms, and, most importantly, the 16 Gramnegative strains were inhibited. Of the 25 isolates, 44.4% of the isolates were able to grow and produce bioactive metabolites when grown in submerged culture. Four extracts showed a cytopathic effect in 10 CCID 50 MDBK cell, even though no viricidal effect was observed. The results obtained with these isolates indicated good biotechnological potential of these Streptomyces strains.

show abstract

Section: Discussionmentioning

confidence: 99%

Preliminary characterization of some Streptomyces species isolated from a composting process and their antimicrobial potential

Salamoni

Mann

Campos

et al. 2010

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Vanillin/sulfuric acid is a non-specific reagent to detect terpenoid or steroid compounds by giving a violet color [23]. Moreover, vanillin/sulfuric acid may also react with alcohols, phenols steroids, then produce red-brown, gray, seldom yellow color on the TLC plate [24]. Thus, the violet spots, especially on Rf 0.97 in Te.234 and Rf 0.37; 0.97 on Te.236 indicated that the extracts contain terpenoid/steroid compounds.…”

Section: Resultsmentioning

confidence: 99%

Isolation of Actinomycetes from Sugarcane (Saccharum officinarum) Rhizosphere and the Ability to Produce Antibiotic.

Aini¹,

Sulistyani²

2019

Proceedings of the 2019 Ahmad Dahlan International Conference Series on Pharmacy and Health Science (ADICS-PHS 2019)

View full text Add to dashboard Cite

Actinomycetes are well-known microorganism as secondary metabolites producers which serve as antibiotics. In this study, sugar cane (Saccharum officinarum) rhizosphere soil samples were collected from Piyungan, Bantul, Yogyakarta. The aims of this study were to obtain Actinomycetes isolates from sugar cane (Saccharum officinarum) rhizosphere which potentially produce antibiotics and to analyse the Thin Layer Chromatography (TLC) profile of ethyl acetate extract of active Actinomycetes isolates. The method used in primary screening to evaluate Actinomycetes antibacterial activity from the sugar cane rhizosphere are agar block method and cup-plate method, and the extract activity assay used Kirby-Bauer method. Determination of antibiotic activity was observed by measuring the diameter of inhibition zone. Extract component was analyzed by Thin Layer Chromatography (TLC). The results of this research was found 39 isolates of Actinomycetes classified into 7 groups based on color grouping. The result of agar block test was revealed that among 39 isolates, four isolates (10.25%) were able to inhibit Staphylococcus aureus. They were Te.234, Te.235, Te.236, and Te.325 isolates. Inhibition zone of crude extracts from these isolates against Staphylococcus aureus were Te.234=7.67 mm, Te.235=3.75 mm, Te.236=12.42 mm, and Te.325=19.16 mm. However, none of these isolates were able to inhibit Escherichia coli. Results of TLC profile showed that the suspected compounds contained in ethyl acetate extract of culture broth are alkaloid, polypeptide, steroid, alcohol, phenol and ketone/aldehyde compounds. Based on the results, it can be concluded that Actinomycetes can be isolated from sugar cane rhizosphere which has potential as an antibiotic producer by narrow spectrum to inhibit Gram-positive bacteria such as Staphylococcus aureus.

show abstract

“…It has been estimated that the genus Streptomyces might produce at least 100,000 new compounds of biological interest (Watve et al 2001). During screening of new isolates for drug discovery, many potentially interesting microorganisms might be excluded due to inadequate techniques, too high selectivities or just because of missing tests (Taddei et al 2006). We decided therefore, to use a so-called horizontal screening, i.e., a combination of tests with low selectivity covering a broad range of antibacterial, antifungal, phytotoxic and cytotoxic activities (Laatsch 2000).…”

Section: Discussionmentioning

confidence: 99%

“…In order to elucidate outstanding natural molecular diversity for drug development, various methods including target-directed biological, physico-chemical and chemical screening strategies have been developed (Broach and Thorner 1996;Grabley et al 1999). Chemical screening using TLC with various staining reagents and HPLC-MS/MS offers the opportunity to visualize a nearly complete picture of a microbial secondary metabolite pattern (metabolic finger-print) (Taddei et al 2006;Burkhardt et al 1996). This approach can be used advantageously for both, the detection of so-called ''talented'' strains (Burkhardt et al 1996), and for qualifying microbial strain collections, especially as a fundamental step of efficiently applied biological high-throughput assays.…”

Section: Introductionmentioning

confidence: 99%

Antifungal and antibacterial activities of indigenous Streptomyces isolates from saline farmlands: prescreening, ribotyping and metabolic diversity

Sajid

Yao

Shaaban

et al. 2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

A culture collection of 110 indigenous Streptomyces strains originally isolated from saline farmlands (Punjab, Pakistan) using stringent methods was screened biologically and chemically to investigate their potential for the production of bioactive secondary metabolites. In a biological screening the crude extracts obtained from the culture broth of selected strains were analysed for their activity against a set of test organisms, including Grampositive, Gram-negative bacteria, fungi and microalgae using the disk diffusion bioassay method. Additionally a cytotoxicity test was performed by means of the brine shrimp microwell cytotoxicity assay. In a chemical screening each of the crude extracts was analysed by TLC using various staining reagents and by HPLC-MS/MS measurements. The results depicted an impressive chemical diversity of crude extracts produced by these strains. The taxonomic status of the selected strains was confirmed by preliminary physiological testing and 16S rRNA gene sequencing.

show abstract

Chemical screening: A simple approach to visualizing Streptomyces diversity for drug discovery and further research

Cited by 14 publications

References 21 publications

Preliminary characterization of some Streptomyces species isolated from a composting process and their antimicrobial potential

Preliminary characterization of some Streptomyces species isolated from a composting process and their antimicrobial potential

Isolation of Actinomycetes from Sugarcane (Saccharum officinarum) Rhizosphere and the Ability to Produce Antibiotic.

Antifungal and antibacterial activities of indigenous Streptomyces isolates from saline farmlands: prescreening, ribotyping and metabolic diversity

Contact Info

Product

Resources

About