Chemical responsiveness and histochemical phenotype of electrophysiologically classified cells of the adult rat dorsal root ganglion

The Endogenous Redox Agent L-Cysteine Induces T-Type Ca2+ Channel-Dependent Sensitization of a Novel Subpopulation of Rat Peripheral Nociceptors

“…The ATP-evoked currents were not only of similar magnitude to those that have been reported in several other nociceptor subpopulations (Petruska et al, 2000(Petruska et al, , 2002 but also had similar desensitization kinetics. Furthermore, T-rich cells are within the size range (Յ35 m) that comprises the majority of the capsaicin-sensitive and ATP-sensitive nociceptor population (Caterina and Julius, 2001;Petruska et al, 2000Petruska et al, , 2002.…”

“…The capsaicin-evoked currents we observed in T-rich cells displayed rapid onset and Ca 2ϩ -dependent desensitization, properties that are characteristic of capsaicin-evoked currents in other DRG cells (Todorovic et al, 2001;Petruska et al, 2000Petruska et al, , 2002, as well as recombinant cells expressing the capsaicin receptor (Caterina et al, 1997). The ATP-evoked currents were not only of similar magnitude to those that have been reported in several other nociceptor subpopulations (Petruska et al, 2000(Petruska et al, , 2002 but also had similar desensitization kinetics. Furthermore, T-rich cells are within the size range (Յ35 m) that comprises the majority of the capsaicin-sensitive and ATP-sensitive nociceptor population (Caterina and Julius, 2001;Petruska et al, 2000Petruska et al, , 2002.…”

“…Binding of IB 4 has been observed in subsets of both A␦-and C-type nociceptors, as well as capsaicin-and ATP-positive cells (Stucky and Lewin, 1999;Petruska et al, 2000Petruska et al, , 2002. Accordingly, we stained T-rich cells with IB 4 after identification on the basis of I-V signature and found that the majority (76%) of T-rich cells (n ϭ 17) were IB 4 positve (Fig.…”

Cell-Specific Alterations of T-Type Calcium Current in Painful Diabetic Neuropathy Enhance Excitability of Sensory Neurons

“…Binding of IB 4 has been observed in subsets of both A␦ -and C-type nociceptors in vivo (Gerke and Plenderleith, 2001), as well as in acutely dissociated medium-size DRG cells (Petruska et al, 2000(Petruska et al, , 2002. However, expression of this marker of nociceptive function in relation to T-type channels and ADP in medium-size neurons has not been studied.…”

Nociceptors: the sensors of the pain pathway

“…Labeling with retrograde dyes injected into the target tissue has enabled characterization of functional attributes of the soma of nociceptors innervating those tissues. The heterogeneity of functional phenotypes observed in isolated sensory cell bodies (46,47) appears to reflect the variability observed in cutaneous nociceptor fiber types observed in studies in which recordings from fiber or soma during receptive field stimulation are combined with subsequent nociceptor labeling to identify terminal morphology (48,49) and the expression of nocisensors (50), markers, and peptides (48) ( Table 1). Nociceptors differentially express a variety of anatomical and biochemical markers (e.g., the expression of versican, the binding partner for the isolectin B4 [IB4]; ref.…”