Chemical reaction during electromigration of ions

Deman, Joris; Rigole, W.

doi:10.1021/j100700a028

Cited by 49 publications

(35 citation statements)

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“…In 1970, Deman et al [19,20] advanced the important concepts of ''precipitate reaction front'' and ''moving reaction front.'' At the same time, Deman [21,22] observed the elution displacement of metal ions and the separation of six rare earth ions in his experiments.…”

Section: Introductionmentioning

confidence: 99%

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Yang¹,

Fan²,

Shan-sheng³

et al. 2011

Electrophoresis

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In this paper, we developed a novel method of acid-base titration, viz. the electromigration acid-base titration (EABT), via a moving neutralization boundary (MNR). With HCl and NaOH as the model strong acid and base, respectively, we conducted the experiments on the EABT via the method of moving neutralization boundary for the first time. The experiments revealed that (i) the concentration of agarose gel, the voltage used and the content of background electrolyte (KCl) had evident influence on the boundary movement; (ii) the movement length was a function of the running time under the constant acid and base concentrations; and (iii) there was a good linearity between the length and natural logarithmic concentration of HCl under the optimized conditions, and the linearity could be used to detect the concentration of acid. The experiments further manifested that (i) the RSD values of intra-day and inter-day runs were less than 1.59 and 3.76%, respectively, indicating similar precision and stability in capillary electrophoresis or HPLC; (ii) the indicators with different pK(a) values had no obvious effect on EABT, distinguishing strong influence on the judgment of equivalence-point titration in the classic one; and (iii) the constant equivalence-point titration always existed in the EABT, rather than the classic volumetric analysis. Additionally, the EABT could be put to good use for the determination of actual acid concentrations. The experimental results achieved herein showed a new general guidance for the development of classic volumetric analysis and element (e.g. nitrogen) content analysis in protein chemistry.

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Section: Introductionmentioning

confidence: 99%

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Yang¹,

Fan²,

Shan-sheng³

et al. 2011

Electrophoresis

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“…(10)(11)(12). Finally, the local concentrations of H 1 in the a phase, C a H þ ðx k Þ;act , can be directly calculated:…”

Section: Local Immobiline Concentrationsmentioning

confidence: 99%

“…It means that the local pH value, local concentrations of acidic and basic Immobilines, pH x k , C Aðx k Þ and C Bðx k Þ , are directly related to a given position, x k ; (ii) Different NaOH concentrations in the rehydration solutions should result in different buffering-titrant cases; thus the different equations, such as Eqs. (10)(11)(12), must be presented to obtain C b OH À ðx k Þ; act . The space-time varying local concentrations should result in the non-uniform velocity motion of MNB.…”

Section: Position-time Pertinencementioning

confidence: 99%

“…Rigehtti et al in 1978 explored the characteristics of CAs clinging to proteins through polyanionic surface of heparin in IPG [10,11]. Deman and Rigole [12,13] in 1970 introduced ''precipitate reaction front'' and ''moving reaction front'' through the moving reaction front experiments of separation of metal ions [14,15]. The electromigration reaction and neutralization boundary were recognized by Murel et al [16], Hayedorn and Fuhr [17] and Pospichal et al [18] to explain the mechanism of the instability of the pH gradient in IEF.…”

Section: Introductionmentioning

confidence: 99%

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Recursion approach for moving neutralization boundary formed on IPG strips Part I: With strong alkali rehydration buffer

Liang¹,

Chen²,

Tian³

et al. 2009

Electrophoresis

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Moving neutralization boundary (MNB) is an important foundation to understand and improve IEF. However, there are obstacles in theoretical predictions of MNB on IPG strips due to the unknown local concentrations of carrier ampholytes on commercial IPG strips and the time-varying boundary velocities. We introduce a recursion approach to extend the current MNB theories into the space-time varying MNB system. The recursion approach emphasizes the localizability of physicochemical parameters in the discrete time intervals and local positions in Lagrangian coordinates, such as local concentrations of carrier ampholytes, local OH concentrations, local boundary velocities, local judgments, etc. The boundary-position recursion equation in a complete time sequence was presented to quantitatively predict the MNB position-time curves by distinguishing three kinds of titration cases according to NaOH concentrations in rehydration buffers. The theoretical position-time curves and local relative judgments of boundaries were satisfactorily validated by corresponding images of boundary migrations achieved from the IPG-MNB experiments with the some typical NaOH concentrations-bromophenol blue-rehydration buffers on pH 4-7 IPG strips. The results achieved herein have evident significances to the development of moving reaction boundary and IEF.

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“…In 1970 Deman and Rigole explored precipitate reaction front and moving reaction front [22,23]. Murel et al [24] dealt with electromigration reaction and neutralization boundary.…”

Section: Introductionmentioning

confidence: 99%

Time‐varying migration process of moving neutralization boundary on the immobilized pH gradient strip in the weak‐base rehydration buffer

Liang

OuYang

Liu

et al. 2011

J of Separation Science

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This paper quantificationally probes into time-varying migration processes of moving neutralization boundary (MNB) on immobilized pH gradient (IPG) strip in ammonia-rehydration buffers. The time-varying migration processes are determined by both time-varying dissociation equilibria of ammonia and position-varying pH environments formed by immobilized carrier ampholytes (CAs) on the IPG strip. Thus, the local dissociation equilibria of ammonia and the position-varying pH are introduced into the recursion equation of position of MNB migrations. The theoretical position-time curves and the velocity-time curves of MNB migrations obtained by the recursion approach were satisfactorily validated by a series of images of boundary migrations from the IPG-MNB experiments by using rehydration buffers with different ammonia concentrations on pH 3-6 IPG strips. The results achieved herein have significant evidence to a quantificational understanding of the mechanism of MNB and IEF.

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Chemical reaction during electromigration of ions

Cited by 49 publications

References 0 publications

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Recursion approach for moving neutralization boundary formed on IPG strips Part I: With strong alkali rehydration buffer

Time‐varying migration process of moving neutralization boundary on the immobilized pH gradient strip in the weak‐base rehydration buffer

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