Chemical Proteomic Approaches Targeting Cancer Stem Cells: A Review of Current Literature

Jung, Hye Jin

doi:10.21873/cgp.20042

Cited by 6 publications

(3 citation statements)

References 103 publications

(85 reference statements)

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“…MS in conjunction with various LC separation techniques (HPLC or UHPLC) and fluorescence detection are very useful for the characterization, identification, and quantification of glycans, giving valuable information about glycan biological functions. − LC–MS analyses have enabled us to identify glycosylation sites, to characterize glycan structures, or to identify or quantify glycoproteins and glycopeptides. Different stationary phases based on lectin, boronate, or TiO 2 affinity, hydrophilic interactions, or hydrazide capture can be used for the selective enrichment of N-glycoproteins, glycans, or glycopeptides. , Moreover, recent LC strategies including C18-RPLC, HILIC, HPAEC, or porous graphitic carbon (PGC) enable the separation of isomeric glycans .…”

Section: Glycoproteomics and Analytical Techniques Usedmentioning

confidence: 99%

“…The steeply growing number of publications related to cancer proteomics and glycoproteomics proves that this is a hot topic (Figure ). A summary of proteomic approaches, advancements, and their applications for profiling analyses of tumor tissues/cell has been published in various reviews, but only a few of them are especially focused on breast cancer. ,,,− …”

Section: Cancer Glycoproteomicsmentioning

confidence: 99%

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Use of Lectin-based Affinity Techniques in Breast Cancer Glycoproteomics: A Review

2020

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Changes in glycoprotein content, altered glycosylations, and aberrant glycan structures are increasingly recognized as cancer hallmarks. Because breast cancer is one of the most common causes of cancer deaths in the world, it is highly urgent to find other reliable biomarkers for its initial diagnosis and to learn as much as possible about this disease. In this Review, the applications of lectins to a screening of potential breast cancer biomarkers published during recent years are overviewed. These data provide a deeper insight into the use of modern strategies, technologies, and scientific knowledge in glycoproteomic breast cancer research. Particular attention is concentrated on the use of lectin-based affinity techniques, applied independently or most frequently in combination with mass spectrometry, as an effective tool for the targeting, separation, and reliable identification of glycoprotein molecules. Individual procedures and lectins used in published glycoproteomic studies of breast-cancer-related glycoproteins are discussed. The summarized approaches have the potential for use in diagnostic and predictive applications. Finally, the use of lectins is briefly discussed from the view of their future applications in the analysis of glycoproteins in cancer.

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Section: Glycoproteomics and Analytical Techniques Usedmentioning

confidence: 99%

Section: Cancer Glycoproteomicsmentioning

confidence: 99%

Use of Lectin-based Affinity Techniques in Breast Cancer Glycoproteomics: A Review

2020

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“…Examples of these pathways include the JAK/STAT, Wnt/β-catenin, Hedgehog, Notch, and TGF-β pathways. 5 , 6 RalA signaling pathways has been shown by our group to be over-activated in a number of human malignancies such as liver, lung, medulloblastoma, malignant peripheral nerve sheath tumors, and ovarian cancer. 7 – 12 According to our data, while the levels of RalA expression are comparable in CSCs and differentiated cancer cells, RalAGTP (the active form of RalA) is at higher levels in CSCs.…”

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confidence: 94%