Chemical properties of bovine cervical mucus during normal estrus and estrus induced by progesterone and/or PGF2α

Tsiligianni, Theodora; Karagiannidis, A.; Brikas, P.; Saratsis, Ph.

doi:10.1016/s0093-691x(01)00541-6

Cited by 23 publications

(20 citation statements)

References 19 publications

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“…The aqueous phase contains 92-95 % of water and several dissolved low-molecular-mass compounds, e.g., ions and metabolites (Tsiligianni et al, 2001); and the gel phase is formed mainly by glycoproteins called mucins (Rutllant et al, 2005;Pluta et al, 2011). Biophysical and biochemical properties of BCM are controlled by changes in sex steroid levels during the oestrous cycle (Rutllant et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Evidence of Fractality in a Pattern of Crystallization of Bovine Cervical Mucus Obtained at Oestrus

et al. 2012

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SUMMARY:Fractality has emerged as a feature of the organization of some complex natural systems. Several biological secretions show fractal-like patterns for their crystallization phenomena but their presence in crystallizations of bovine cervical mucus (BCM) is yet unknown. In order to assess the fractality of BCM crystallization, samples of this fluid were taken from heifers at oestrus, their crystalline patterns photographed and its morphology analyzed. Among the many images obtained for BCM crystallizations, one of them had a highly symmetrical geometric arrangement, possessing three zones characterized by pine-like, arboriform structures, evidencing a remarkable similarity between them. Moreover, fractal dimensions obtained for these zones were statistically equal when analyzed by using specialized software. In summary, this brief communication shows, for the first time, that a pattern of crystallization of BCM at oestrus possesses a fractal-like organization.

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Section: Introductionmentioning

confidence: 99%

Evidence of Fractality in a Pattern of Crystallization of Bovine Cervical Mucus Obtained at Oestrus

et al. 2012

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“…Both physical and biochemical characteristics of cervical mucus are changed under hormonal‐induced estrus (Tsiligianni et al . ,b). Total protein level of the cervical mucus stimulated by PGF 2 α was higher compared to natural estrus (Yildiz & Aydin ).…”

Section: Discussionmentioning

confidence: 99%

“…Glucose appears to be one of the major free sugars that have been identified in cervical mucus of cattle (Tsiligianni et al . 2001b). It is believed that lipids play a nutritive role for spermatozoa, and they also protect spermatozoa from temperature changes (Nasrin & Calogero ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of three hormonal protocols for anovulatory lactating cows under regulations restricting the use of estrogenic compounds

Wang

et al. 2018

Animal Science Journal

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When European Union regulations restricted the use of estrogenic compounds in food-producing animals, refined hormonal protocols were no longer applicable for anovulatory cows. However, Ovsynch and its adaptations are routinely and uniformly applied to all cows regardless of ovarian function. To evaluate their efficacy on anovulatory cows, 143, 147 and 144 anovulatory cows received Ovsynch, Presynch and G6G protocols, respectively. In comparison, 150 cyclic cows were bred without using a synchronized protocol. Results showed that cows in the Presynch group had luteolysis responding to the last prostaglandin F (PGF ) injection greater than the Ovsynch group. The serous progesterone levels at the first gonadotropin-releasing hormone of Ovsych and the last PGF injection was greater in the G6G group than the other two hormonal treatment groups. Concentrations of Ca and total protein in cervical mucus in all three hormone-treated groups before artificial insemination (AI) were significantly different from the controls. The G6G group obtained a greater pregnancy rate compared with Ovsynch and Presynch, but significantly less than the controls. For open cows in the Ovsynch group, estrus rate within 24 days after the first AI was significantly less than the controls. In conclusion, the G6G treatment resulted to better reproductive performance in anovulatory cows.

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“…HA is localized to the stromal ECM (El Maradny et al 1997), and its biological role in cervical remodelling is hypothesized but not well defined. Because cervical relaxation at oestrus is accompanied by an increase in water content (Tsiligianni et al 2001) and HA has a high affinity for water molecules (Takemura et al 2005), a proposed role for HA in cervical remodelling is thought to be in promotion of tissue hydration. The accumulation of HA and water molecules in the interstices between the collagen fibrils may promote dispersion or prevent aggregation of the collagen fibrils, thus weakening the tensile strength of the matrix (El Maradny et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan (HA) content, the ratio of HA fragments and the expression of CD44 in the ovine cervix vary with the stage of the oestrous cycle

Perry¹,

Haresign²,

Wathes³

et al. 2010

Reproduction

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The complex anatomy of the ovine cervix limits the success of trans-cervical artificial insemination in sheep. However, there is a degree of natural relaxation of cervix at oestrus that is accompanied by an increase in the water content. As hyaluronan (HA) has a high affinity for water molecules, in this study, we tested the hypothesis that the HA content of the cervix, the proportion of different size fragments of HA and expression of its receptor CD44 vary with the stage of the oestrous cycle. Oestrous was synchronized in 25 Welsh mountain ewes, and their cervices were collected either during luteal phase (nZ8) or pre-LH (nZ8) or post-LH (nZ9) surge stage of the oestrous cycle. The pre-LH surge group had the highest HA content (2.96 ng/mg of cervical tissue), which was significantly (P%0.05) higher than that observed for the post-LH surge (2.04 ng/mg) group. The luteal phase group had a mean HA content intermediate between the pre-and post-LH surge groups, and was significantly different from either. The frequency of cervical samples containing both sizes of HA fragments (small and large) was significantly higher (P%0.05) in the pre-LH surge group compared with the luteal and the post-LH surge groups, whereas that in post-LH surge group was significantly (P%0.05) higher than that in the luteal group. The number of cervical samples that contained only small HA fragments was significantly (P%0.05) higher in the luteal group compared with both the pre-and post-LH surge groups, whereas the number of samples containing only large HA fragments was significantly (P%0.05) higher in the post-LH surge group compared with the luteal or pre-LH surge groups. Overall mean expression of CD44 in the vaginal and mid regions was significantly (P%0.001) higher than that in the uterine region, with no difference between the vaginal and mid regions of the cervix. Pattern of CD44 expression depended on the stage of the oestrous cycle. At the luteal stage, CD44 expression did not vary among epithelial, sub-epithelial, circular and longitudinal smooth muscle layers, whereas at the pre-and post-LH surge stages, the expression in the epithelial layer was significantly (P%0.001) higher than that in the other three layers. In general, CD44 expression in the transverse smooth muscle layer was significantly (P%0.05) lower than the expression in all the other layers at all the stages of the oestrous cycle. The results indicated that the HA varied with the steroid status. Higher HA values at a time when cervical relaxation is naturally higher may indicate its involvement in remodelling of the cervix at oestrus.

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Chemical properties of bovine cervical mucus during normal estrus and estrus induced by progesterone and/or PGF2α

Cited by 23 publications

References 19 publications

Evidence of Fractality in a Pattern of Crystallization of Bovine Cervical Mucus Obtained at Oestrus

Evidence of Fractality in a Pattern of Crystallization of Bovine Cervical Mucus Obtained at Oestrus

Evaluation of three hormonal protocols for anovulatory lactating cows under regulations restricting the use of estrogenic compounds

Hyaluronan (HA) content, the ratio of HA fragments and the expression of CD44 in the ovine cervix vary with the stage of the oestrous cycle

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