Chemical profiling and cytotoxic activity of 150-year old original sample of Jerusalem Balsam

Łyczko, Jacek; Pawlak, Aleksandra; Augustyński, Iwo; Okińczyc, Piotr; Szperlik, Jakub; Kulma, Anna; Różański, Henryk; Obmińska-Mrukowicz, Bożena; Szumny, Antoni

doi:10.1016/j.fct.2020.111183

Cited by 8 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The profile of the essential oils investigated was evaluated using the GC-MS technique, according to the protocol [ 66 ]. The identification of all of the volatile components was based on a comparison of the mass spectra with the mass spectra of the compound obtained experimentally, available in the NIST20 database.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Evaluation of Antiprotozoal Properties, Cytotoxicity Effect and Anticancer Activity of New Essential-Oil Based Phytoncide Mixtures

et al. 2023

Self Cite

View full text Add to dashboard Cite

Protozoa, in both humans and animals, are one of the leading causes of disease. International programmes introduced in many countries have helped reduce the incidence of disease. However, it has recently become increasingly difficult to achieve the goals set for the coming years. One of the main reasons for this, as with other pathogenic organisms, such as bacteria and fungi, is the increasing resistance to current methods of treating and preventing infection. Therefore, new therapies with high efficacy are needed. In the present study, the novel mixtures of essential oils (EOs), clove, garlic, Ceylon cinnamon, and rosemary with organic acids (acetic, propionic, lactic) and metal ions (Cu, Mn, Zn) were tested against five selected model protozoa (Euglena gracilis, Gregarina blattarum, Amoeba proteus, Paramecium caudatum, Pentatrichomonas hominis). The cytotoxicity and potential anticancer activity of the obtained combinations were tested on the human fibroblasts (NHDF) and human cancer cell lines (A549, MCF7, LoVo, HT29). All of the mixtures showed very good antiprotozoal properties. The most efficient were the combination of clove and rosemary essential oils, mixtures of acids, and Mn ions. The LD50 values were in the range of 0.001–0.006% and the LD100 values were 0.002–0.008%. All of the tested mixtures did not show cytotoxicity against normal cells, but did show growth inhibition against cancer cell lines. The most cytotoxic against cancer cells were combinations with cinnamon essential oil. Nevertheless, the proposed combinations containing essential oils, organic acids, and metal ions have high antiprotozoal activity, with low toxicity to healthy human cells.

show abstract

Section: Methodsmentioning

confidence: 99%

In Vitro Evaluation of Antiprotozoal Properties, Cytotoxicity Effect and Anticancer Activity of New Essential-Oil Based Phytoncide Mixtures

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…The analysis of volatile composition of phytoncides, presented in this paper were determined by the GC-MS technique according to the protocol [ 56 ]. The identification of all volatile constituents for untargeted analysis (full-scan mode) was based on the comparison of the mass spectra of the compound obtained experimentally with the mass spectra available in the NIST20 database.…”

Section: Methodsmentioning

confidence: 99%

The Impact of a Phytobiotic Mixture on Broiler Chicken Health and Meat Safety

Iwiński,

Chodkowska,

Drabik

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

The purpose of the study was to assess the effects of different doses of a phytobiotic mixture on selected production parameters and meat quality and to assess the residue of the preparation in tissues and the possible toxic effects in broiler chickens. Broiler chicks aged 160 days, divided into four equal groups, were supplemented with the phytobiotic mixture at different doses, D1—0.5 mL/L, D2—1 mL/L, and D3—2 mL/L, four times during a 42-day trial. There were no statistically significant differences in weight gain per week of life and mortality in the birds. The study also demonstrated that the use of the mixture of phytobiotics had no significant effect on colour, pH, WHC, and natural leakage. However, a beneficial effect of the additive was found in the group treated with a dose of 1 mL/L, where less thermal leakage from the meat was demonstrated. Furthermore, significant differences in the change in thigh muscle tenderness were also observed. In the histopathological analysis of the liver no significant differences were observed. In addition, no residues of the mixture or its metabolites were found in the tissues analysed. In conclusion, the proposed scheme of administration of the phytobiotic additive, regardless of the dose, does not cause pathological changes in organs and does not carry the risk of residues of the product in tissues intended for human consumption.

show abstract

“…The profile of the essential oils investigated was assessed using the GC-MS technique according to the protocol [ 78 ]. Identification of all volatile constituents was based on comparison of experimentally obtained compound’s mass spectra with mass spectra available in the NIST20 database.…”

Section: Methodsmentioning

confidence: 99%

Novel Formula of Antiprotozoal Mixtures

et al. 2022

Self Cite

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is becoming more common in both bacteria and pathogenic protozoa. Therefore, new solutions are being sought as alternatives to currently used agents. There are many new ideas and solutions, especially compounds of natural origin, including essential oils. In the present study, the antiprotozoal activity of a mixture of essential oils (eucalyptus, lavender, cedar and tea tree), organic acids (acetic acid, propionic acid and lactic acid) and metal ions (Cu, Zn, Mn) were tested. As a model, protozoans were selected: Euglena gracilis, Gregarina blattarum, Amoeba proteus, Paramecium caudatum, Pentatrichomonas hominis. The tested concentrations of mixtures were in the range of 0.001–1.5%. The analyses show unexpected, very strong protozoicidal activity of combinations, presenting the synergy of compounds via determination of LD50 and LD100 values. Obtained mixtures showed significantly higher activity against protozoans, compared to chloramphenicol and metronidazole. Most of the analyzed samples show high antiprotozoal activity at very low concentration, in the range of 0.001–0.009%. The most effective combinations for all analyzed protozoans were the cedar essential oil and tea tree essential oil with a mixture of acids and manganese or zinc ions. Innovative combinations of essential oils, organic acids and metal ions are characterized by very high antiprotozoal activity at low doses, which, after further investigation, can be applicable for control of protozoan pathogens.

show abstract

Chemical profiling and cytotoxic activity of 150-year old original sample of Jerusalem Balsam

Cited by 8 publications

References 42 publications

In Vitro Evaluation of Antiprotozoal Properties, Cytotoxicity Effect and Anticancer Activity of New Essential-Oil Based Phytoncide Mixtures

In Vitro Evaluation of Antiprotozoal Properties, Cytotoxicity Effect and Anticancer Activity of New Essential-Oil Based Phytoncide Mixtures

The Impact of a Phytobiotic Mixture on Broiler Chicken Health and Meat Safety

Novel Formula of Antiprotozoal Mixtures

Contact Info

Product

Resources

About