Chemical profiling and antioxidant evaluation of Yangxinshi Tablet by HPLC–ESI-Q-TOF-MS/MS combined with DPPH assay

Zhu, Junfeng; Yi, Xiaojiao; Zhang, Jinghui; Shuqing, Chen; Wu, Yao

doi:10.1016/j.jchromb.2017.06.022

Cited by 49 publications

(32 citation statements)

References 35 publications

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“…Therefore, it is of great importance to develop a rapid and accurate technique to determine the specific ability of individual active compounds in the natural extracts of complexes. High-performance liquid chromatography (HPLC) analysis combined with DPPH and/or ABTS assays are currently implemented for rapid screening of active compounds in extracts 21,22 .…”

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confidence: 99%

“…In DPPH/ABTS-guided HPLC analysis, after a specific antioxidant in the extract reacts with DPPH• or ABTS• + , one or more hydrogen atoms from the former are transferred to the latter, leading to a decrease in, or extinction of, the antioxidant peak compared to that in the HPLC profile of the unreacted extract 21 . In total, 127 compounds have been identified from the so-called 'Yangxinshi Tablet' and 34 antioxidant compounds were rapidly screened using an online DPPH-guided HPLC assay 22 . In another study, an offline DPPH-guided HPLC assay was applied to a Brazilian green propolis extract to screen nine DPPH• scavengers from among 14 characteristic peaks in the HPLC profiles 23 .…”

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confidence: 99%

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Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Cao

Yang

Xue

et al. 2020

Sci Rep

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Morus and Broussonetia trees are widely used as food and/or feed. Among 23 phenolics identified from leaves of five Moraceae species using UPLC-QTOF-MS/MS, 15 were screened using DPPH/ABTSguided HPLCs, including seven weak (flavonoids with one hydroxyl on B-ring) and eight strong (four caffeoylquinic acids and four flavonoids, each with a double hydroxyl on B-ring) antioxidants. We then determined the activity and synergistic effects of individual antioxidants and a mixture of the eight strongest antioxidants using DPPH-guided HPLC. Our findings revealed that (1) flavonoid glucuronide may have a more negative effect on antioxidant activity than glucoside, and (2) other compounds in the mixture may exert a negative synergistic effect on antioxidant activity of the four flavonoids with B-ring double hydroxyls but not the four caffeoylquinic acids. In conclusion, the eight phenolics with the strongest antioxidant ability reliably represented the bioactivity of the five extracts examined in this study. Moreover, the Morus alba hybrid had more phenolic biosynthesis machinery than its cross-parent M. alba, whereas the Broussonetia papyrifera hybrid had significantly less phenolic machinery than B. papyrifera. This difference is probably the main reason for livestock preference for the hybrid of B. papyrifera over B. papyrifera in feed.Morus and Broussonetia tree species (family: Moraceae) have high economic value; among other uses, their leaves are widely used as feed to improve meat quality. The genus Broussonetia comprises five species: B. papyrifera, B. kazinoki, B. zeylanica, B. kaempferi, and B. kurzii 1 , some of which are used as folk medicinal plants and/or for making bark paper 2 . The bark, fruits, leaves, and roots of B. papyrifera and B. kazinoki exhibit antioxidant, anti-inflammatory, antinociceptive, and anti-tyrosinase activity 3-6 . In China, B. papyrifera and B. kazinoki leaves, from which several bioactive components such as polyphenols, flavonoids and alkaloids have been isolated 7 , are widely used to feed livestock for meat quality improvement. Methanol extracts from B. papyrifera leaves have greater inhibitory effects on mushroom tyrosinase and 2,2-diphenyl-1-picrylhydrazyl/(DPPH)• scavenging activity 8 , and ethanol extracts from B. papyrifera roots inhibit both acetyl-and butyl-cholinesterases 2 . Papyriflavonol A, isolated from the root bark of B. papyrifera, exhibits strong antimicrobial activity against different pathogenic bacteria and fungi 9 , whereas a 70% ethanol leaf extract of B. kazinoki was reported to dramatically reduce ear and dorsal skin thickness and other clinical symptoms in mice with atopic dermatitis, possibly via the downregulation of immunoglobulin E (IgE) and interleukin-4 (IL-4) plasma levels 5 . Broussonone A extracted from B. kanzinoki stem bark displays noncompetitive inhibitory activity against pancreatic lipase 10 . However, data on the antioxidant activity and bioactive composition of B. kanzinoki remains limited.The genus Morus comprises 10-16 species 11 , of whic...

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confidence: 99%

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Cao

Yang

Xue

et al. 2020

Sci Rep

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“…The other compounds were tentatively characterized based on the mass accuracy of their precursor ions (within a 5 ppm error), tandem MS spectra, and fragmentation pathways, referring to the previous literature. [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] Aer a thorough literature search, 2 compounds, comprising 1 avonoid (isoliquiritigenin-4 0 -apiosyl(1/2)glucoside) and 1 saponin (apioglycyrrhizin or araboglycyrrhizin), were rst identied in CLT in the present study. Chalcones and avanones.…”

Section: Resultsmentioning

confidence: 99%

“…25 CLT is an important TCM prepared from ve medicinal herbs, including licorice root (extract) concrete (LRC), powdered poppy capsule extractive (PPCE), camphor, oleum anisi stellati, and sodium benzoic (SMB), which have been widely used for anti-inammatory, antitussive, expectorant, and antiasthmatic actions. 26 The compositions of CLT mainly include avonoids, saponins, alkaloids, and other compounds, as reported in previous reports, [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] and these chemical components have a common pharmacological activity, that is, their antioxidant activity. [45][46][47] Moreover, the published studies have shown that the antioxidant components, especially avonoids, saponins, and alkaloids of herbal products, can decrease the risk of numerous diseases, such as inammation, senescence cardiovascular disease, neurodegenerative disorders, diabetes, and cancer, closely related to the damaging effects of free radicals.…”

Section: Introductionmentioning

confidence: 90%

UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

et al. 2018

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“…[1][2][3][4][5] Natural products from fruits and plants are proved to have antioxidant properties by neutralizing excessive free radicals, reducing inflammation and oxidative stress. 4,6 Considering health risks of synthetic antioxidants, especially cancer, there is increasing interest in replacing them with natural ones, which are generally of botanical origin. 7 The genus Ficus belongs to the Moraceae family and comprises about 1000 species around the world, which are distributed in the tropical to subtropical areas, and 98 species occur in China.…”

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Improvement Effect of Ficus vasculosa Ethanol Extract on D-galactose-Induced Mice Aging

Song

2019

Natural Product Communications

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This study was to investigate antioxidant activities of the ethanol extract from young edible leaves of Ficus vasculosa in vitro and in vivo. Ficus vasculosa ethanol extract (FVEE) showed significantly higher reducing power and α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenge activity than vitamin C (P < 0.05). FVEE also showed an activity to resist the D-galactose-induced aging in mice assessed by serum and tissue levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Total serum and tissue oxidative status, total antioxidantresponse, glutathione (GSH) and malondialdehyde (MDA) levels have been also measured. Pretreatment with FVEE at 200 mg/kg·body weight significantly increased enzyme activity of SOD and CAT in serum and hepatic tissue (P < 0.05), as well as significantly increased enzyme activity of SOD in kidney (P < 0.05). Furthermore, high concentration of FVEE pretreatment significantly increased the level of GSH in serum, hepatic tissue and kidney (P < 0.05), meanwhile significantly decreased MDA production in hepatic tissue and kidney (P < 0.05). In addition, the phytochemical investigation discovered six previously described compounds from FVEE, naringenin (1), vanillic acid (2), 9, 16-dioxo-10, 12, 14-octadeca-trienoic acid (3), 2, 6-dimethoxy-1, 4-benzoquinone (4), apigenin (5) and norartocarpetin (6), and all compounds were isolated from this plant for the first time. Among the various compounds found, the rare highly unsaturated fatty acid 9, 16-dioxo-10, 12, 14-octadeca-trienoic acid (3) has been identified, which had been isolated only once before from F. vasculosa. Evaluation of the antioxidant activity of isolated compounds showed naringenin (1) to be the most active. According to our research, FVEE present very high antioxidant activity in vitro due to the presence of several compounds known for their antioxidant activity such as flavonoid and phenolic acid. In vivo, the ethanol extract had improvement effects against D-galactose-induced aging by reducing oxidative stress.

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Chemical profiling and antioxidant evaluation of Yangxinshi Tablet by HPLC–ESI-Q-TOF-MS/MS combined with DPPH assay

Cited by 49 publications

References 35 publications

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

Improvement Effect of Ficus vasculosa Ethanol Extract on D-galactose-Induced Mice Aging

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