Chemical Profile of Malva Neglecta and Malvella Sherardiana by Lc- MS/MS, GC/MS and Their Anticholinesterase, Antimicrobial and Antioxidant Properties With Aflatoxin-Contents

Abstract

The purpose of the present work was to calculate the phenolic and the fatty acids profiles by LC-MS/MS and GC/MS with their antioxidant, anticholinesterase and antimicrobial activities, and aflatoxin contents of Malva neglecta and Malvella sherardiana. The phenolic contents of M. neglecta and M. sherardiana were determined by LC-MS/MS. Malic and 4-OH benzoic acids were found to be the most abundant compounds in M. neglecta and M. sherardiana, respectively. On the other hand, essential oils and fatty acids composition were determined by GC/MS analysis. The methanol extracts of the plants showed the highest effect in all antioxidant assays of this study. The methanol extract of M. neglecta showed the highest activity among the petroleum ether, acetone, methanol and water extracts against acetyl-and butyryl-cholinesterase enzymes (53.68% and 63.95% inhibition ratio, respectively). The acetone extracts of M. neglacta and M. sherardiana exhibited moderate activity against C. albicans with 18 and 17 mm inhibion zone diameter, respectively. The results of the present study is also showed that the M. neglecta and M. sherardiana can also be used as a food source due to its high phenolic acid content and good antioxidant property. Also, the samples were aflatoxin free.

Malva species: Insights on its chemical composition towards pharmacological applications

“…The GC and GC/Ms analyses of mature M. aegyptiaca revealed hexadecanoic acid (20.75%), ethyl linoleolate (18.76%), phytol (11.92%) and neophytadiene (5.15%), terpenyl acetate (3.76%), caryophyllene oxide (3.72%), tetradecanoic acid (3.35%), and 6,10,14-trimethyl-2pentadecanone (3.24%) as major components (Table 1; Zouari et al, 2011). The hierarchical cluster analysis of Malva species based on relative percentage areas their essential oil constituents revealed likeness FIGURE 1 Distribution of Malva species (CalPhotos, 2012) [Colour figure can be viewed at wileyonlinelibrary.com] TABLE 1 Chemical composition (%) of the essential oil from Malva species (Al-Rubaye, Kaizal, & Hameed, 2017;Haşimi et al, 2017;Zouari et al, 2011)…”

“…The essential oils composition of M. neglecta gas chromatography/flame‐ionization detection (GC/FID) and GC/mass spectrometry (MS) analyses revealed 41 components with cineole (18.8%), hexatriacontane (7.8%), tetratetracontane (7.8%), and α‐selinene (4.2%) as the main constituents (Tables and ; Haşimi et al, ). Many reports reveal different concentrations of volatile compounds, macronutrients, and metal ions according to the three growth stages of M. aegyptiaca L. (Zouari et al, ).…”

A network pharmacology approach to assess Reno-protective and -curative effects of methanolic extract of Malva neglecta Wallr in gentamicin induced renal toxicity rat model

“…Literature showed presence of phenolic compounds named quinic acid, malic acid, tr-aconitic acid, protocatechuic acid, tr-caffeic acid, p-coumaric acid, 4-hydroxy benzoic acid and salicylic acid. 4-hydroxy benzoic acid was found to be most abundant among all (amount phenolic compouds) quantification of phenolic compounds (Hasimi et al, 2017). Traditionally, MN is used in food as vegetable and fruit (Ghanati, Khatami, 2011;Dalar, Turker, Konczak, 2012;Rahim et al, 2016;Saremi, Kargar, Pourahmadi, 2015).…”

Preparation, Characterization and Antibacterial Activity of Malva Sylvestris L. Seed Extract Containing Novel Electrospun PVA Nanofibers

“…By using accutare mass Q-TOF LC-MS spectrometer, more than 20 metabolites were characterized from M. Sylvestris L. extracts as in the literature reported previously [31][32][33][34]. The molecular ion [M-H]and MS-Fragment ion m/z values were listed in Table 1.…”