Chemical profile of craft brewer’s spent yeast and its antioxidant and antiproliferative activities

Horn, Priscila Aparecida; Zeni, Ana Lúcia Bertarello; Cavichioli, Natália; Winter, Evelyn; Batista, Keila Zaniboni Siqueira; Vitali, Luciano; Almeida, Eduardo Alves de

doi:10.1007/s00217-023-04268-z

Cited by 4 publications

(7 citation statements)

References 98 publications

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“…The cells were cultured in 25 mM glucose or mannitol for 72 h. Subsequently, the cells (5 × 10 3 ) were treated with indicated concentrations of 2HH (0 [control], 0 [high BS], 10 [high BS + low-dose 2HH], and 20 [high BS + high-dose 2HH] μM) for 24 h. Following this treatment, the cells were exposed to 0.5 mg/mL resazurin (Sigma-Aldrich, Burlington, MA, USA) for 48 h. Then, the cells were lysed using DMSO, and the absorbance was measured at 560-590 nm. (Horn et al, 2023) Due to low proliferative rate of CAC, a flowcytometry (Becton Dickinson) was employed to assess the viability. The CAC was incubated in 25 mM glucose or mannitol for 72h.…”

Section: Proliferation and Viabilitymentioning

confidence: 99%

2-Hydroxyl hispolon reverses high glucose induced endothelial progenitor cell dysfunction through the PI3K/AKT/eNOS and AMPK/HO1 pathways

Wang,

Hou,

Yang

et al. 2024

Preprint

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Background and Purpose: In diabetes, elevated blood sugar (BS) levels contribute to the overproduction of reactive oxygen species (ROS), leading to endothelial progenitor cell (EPC) dysfunction. 2-hydroxy hispolon (2HH), a derivative of Hispolon (HIS), shows potential in reversing high glucose-induced EPC dysfunction. Experimental Approach: In vitro, we inspected the effect of 2HH on three different angiogenic cells, including outgrowth endothelial cell (OEC), circulating angiogenic cell (CAC) and endothelial cell (EC). In vivo, High-fat diet and streptozotocin induced diabetic mice and hindlimb ischemic model were utilized to evaluate the effect of 2HH on angiogenesis and CAC mobilization. Key Results: Treatment with 2HH significantly improved the proliferation, migration, and tube formation, NO synthesis and ROS diminishing of OEC under high glucose conditions. Moreover, 2HH administration potentiates the AMP-activated protein kinase (AMPK)/heme oxygenase-1 (HO-1) and phosphoinositide 3-kinases (PI3K)/protein kinase B (AKT)/endothelial nitric oxide synthase (eNOS) signaling pathways in OECs exposed to high glucose levels. Additionally, 2HH reverses high glucose-induced impairment of CAC function but fails to restore EC dysfunction under high glucose conditions. Hindlimb ischemic assay demonstrated that 2HH treatment enhances blood flow recovery in ischemic hindlimbs and increases the number of circulating CACs in diabetic mice after femoral ligation. Conclusion and Implications: Our study has demonstrated that 2HH effectively reduces oxidative stress, thereby preventing EPC dysfunction induced by high glucose levels, primarily through the PI3K/AKT/eNOS and AMPK/HO-1 pathways. These findings offer a promising therapeutic avenue for attenuating the susceptibility to critical limb ischemia in patients with diabetes.

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Section: Proliferation and Viabilitymentioning

confidence: 99%

2-Hydroxyl hispolon reverses high glucose induced endothelial progenitor cell dysfunction through the PI3K/AKT/eNOS and AMPK/HO1 pathways

Wang,

Hou,

Yang

et al. 2024

Preprint

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“…Unlike pure cultured yeast, BSY is obtained after approximately 1 week of fermentation, during which composed of active and inactive yeast cells (Horn et al, 2023). Additionally, BSY is mixed with wort and hop juice during fermentation.…”

Section: Proximate Compositionmentioning

confidence: 99%

“…Yeast, a single-celled microorganism belonging to the fungal kingdom, has long been recognized for its pivotal role in various industrial processes, most notably in the production of alcoholic beverages such as beer (Bastos et al, 2022;Ferreira, Pinho, Vieira, & Tavarela, 2010;Horn et al, 2023). Beyond its fermentation application, yeast has garnered scientific interest due to its rich composition, encompassing not only proteins extracted in the form of yeast extract but also valuable polysaccharides residing within its cell wall (Bastos et Zhang et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Composition and Soluble Polysaccharide Content between Brewer’s Spent Yeast and Cultured Yeast Cells

Lee,

Park,

Chewaka

2024

Preprint

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Yeast, crucial in beer production, holds promise as a valuable by-product resource, known as brewer's spent yeast (BSY), with potentially beneficial physiological effects. This study aimed to compare the composition and soluble polysaccharide content of Brewer's spent yeast with those of pure cultured yeast strains, namely Saccharomyces cerevisiae (SC) and S. boulardii (SB), to facilitate the utilization of BSY as an alternative source of functional polysaccharides. BSY exhibited significantly higher carbohydrate content and lower crude protein content than SC and SB cells. The residues recovered through autolysis were 53.11%, 43.83%, and 44.99% for BSY, SC, and SB, respectively. Notably, the polysaccharide content of BSY residue (641.90 μg/mg) was higher than that of SC (553.52 μg/mg) and SB (591.56 μg/mg). The yields of alkali-extracted water-soluble polysaccharides were 40%, 30%, and 35% for BSY, SC, and SB, respectively, with BSY comprising a higher proportion of water-soluble saccharides than SC and SB, including 49.3% mannan and 20.1% β-glucan. Furthermore, BSY demonstrated antioxidant activities, including superoxide dismutase (SOD), ABTS, and DPPH scavenging potential, suggesting its ability to mitigate oxidative stress. BSY also exhibited a significantly higher total phenolic compound content, indicating its potential as an effective functional food material.

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“…Yeast, a single-celled microorganism belonging to the fungal kingdom, has long been recognized for its pivotal role in various industrial processes, most notably in the production of alcoholic beverages such as beer [1][2][3]. Beside its application in fermentation processes, yeast has garnered scientific interest due to its rich composition, encompassing not only proteins extracted in the form of yeast extract, but also valuable polysaccharides residing within its cell wall [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Yeast cell walls are a source of useful polysaccharides with a range of biological activities and uses in medicine [1,3,[6][7][8][9]. Past research has clarified the complex structure and composition of Saccharomyces cerevisiae cell walls, demonstrating that they make up 15-35% of the dry weight of the cell and are composed of up to 90% polysaccharides, organized in layers within the cell wall system [10].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Composition and Soluble Polysaccharide Content between Brewer’s Spent Yeast and Cultured Yeast Cells

Lee,

Park,

Chewaka

2024

Foods

View full text Add to dashboard Cite

Yeast, crucial in beer production, holds great potential owing - to its ability to transform into a valuable by-product resource, known as brewer’s spent yeast (BSY), with potentially beneficial physiological effects. This study aimed to compare the composition and soluble polysaccharide content of Brewer’s spent yeast with those of cultured yeast strains, namely Saccharomyces cerevisiae (SC) and S. boulardii (SB), to facilitate the utilization of BSY as an alternative source of functional polysaccharides. BSY exhibited significantly higher carbohydrate content and lower crude protein content than SC and SB cells. The residues recovered through autolysis were 53.11%, 43.83%, and 44.99% for BSY, SC, and SB, respectively. Notably, the polysaccharide content of the BSY residue (641.90 μg/mg) was higher than that of SC (553.52 μg/mg) and SB (591.56 μg/mg). The yields of alkali-extracted water-soluble polysaccharides were 33.62%, 40.76%, and 42.97% for BSY, SC, and SB, respectively, with BSY comprising a comparable proportion of water-soluble saccharides made with SC and SB, including 49.31% mannan and 20.18% β-glucan. Furthermore, BSY demonstrated antioxidant activities, including superoxide dismutase (SOD), ABTS, and DPPH scavenging potential, suggesting its ability to mitigate oxidative stress. BSY also exhibited a significantly higher total phenolic compound content, indicating its potential to act as an effective functional food material.

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Chemical profile of craft brewer’s spent yeast and its antioxidant and antiproliferative activities

Cited by 4 publications

References 98 publications

2-Hydroxyl hispolon reverses high glucose induced endothelial progenitor cell dysfunction through the PI3K/AKT/eNOS and AMPK/HO1 pathways

2-Hydroxyl hispolon reverses high glucose induced endothelial progenitor cell dysfunction through the PI3K/AKT/eNOS and AMPK/HO1 pathways

A Comparative Study of Composition and Soluble Polysaccharide Content between Brewer’s Spent Yeast and Cultured Yeast Cells

A Comparative Study of Composition and Soluble Polysaccharide Content between Brewer’s Spent Yeast and Cultured Yeast Cells

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