Chemical profile of Cimicifuga heracleifolia Kom. And immunomodulatory effect of its representative bioavailable component, cimigenoside on Poly(I:C)-induced airway inflammation

Hu, Linlin; Song, Xiaojun; Nagai, Takayuki; Yamamoto, Marina; Dai, Yi; He, Liangliang; Kiyohara, Hiroaki; Yao, Xin‐Sheng; Yao, Zhihong

doi:10.1016/j.jep.2020.113615

Cited by 18 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We provide new ideas and methods for quality control and modern analysis of TCM. Finally, a total of 120 compounds were identified in SXT (Table S1) [20][21][22][23][24][25][26][27][28]. Twelve of them were unambiguously identified by comparison with reference compounds.…”

Section: Resultsmentioning

confidence: 99%

A multidimensional strategy to rapidly identify the chemical constituents in Shengxian Decoction by using ultra‐performance liquid chromatography coupled with ion mobility spectrometry quadrupole time‐of‐flight mass spectrometry

Wang

Gao

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

As a well‐known traditional Chinese medicine formula, the chemical constituents of Shengxian Decoction still remain unclear due to its complexity. In this study, a multidimensional strategy based on ultra‐performance liquid chromatography coupled with ion mobility spectrometry quadrupole time‐of‐flight mass spectrometry and informatics UNIFI platform was applied to achieve rapid and comprehensive identification of the complex composition of Shengxian Decoction. Data‐independent acquisition, fast data‐directed analysis, and high‐definition MSE were used to obtain more and cleaner mass spectrum information. As a result, a total of 120 compounds including 74 saponins, 17 flavonoids, 7 cinnamic acid derivatives, 8 triterpenoids, and 14 others were identified or tentatively characterized by high‐resolution molecular mass, fragment ions, and collision cross‐section values. Furthermore, high‐definition MSE was used to identify six pairs of co‐eluting isomers that could not be detected from conventional data‐independent acquisition and fast data‐directed analysis. This research strategy has a certain potential for the analysis of other compound formulae and lays the foundation for the study of traditional Chinese medicine efficacy.

show abstract

Section: Resultsmentioning

confidence: 99%

A multidimensional strategy to rapidly identify the chemical constituents in Shengxian Decoction by using ultra‐performance liquid chromatography coupled with ion mobility spectrometry quadrupole time‐of‐flight mass spectrometry

Wang

Gao

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Then, by comparing the retention time and the accurate relative molecular mass, the structure of the detected chemical composition is determined. In addition, we combined our laboratory’s previous experience in the isolation and identification of cimicifuga and summarized the fragmentation regularities of these compounds by mass spectrometry [ 10 , 17 , 18 ]; some compounds were determined by comparison with the mass spectrometry data of the reference substance. Figure 1 shows the UHPLC-Q-TOF-MS base peak intensity chromatograms of the rhizomes and fibrous roots of C. dahurica in positive and negative electrospray (ESI) modes.…”

Section: Resultsmentioning

confidence: 99%

Fibrous Roots of Cimicifuga Are at Risk of Hepatotoxicity

et al. 2022

View full text Add to dashboard Cite

The cause of liver damage by using black cohosh preparation has been concerned but remains unclear. After a preliminary investigation, the black cohosh medicinal materials sold in the market were adulterated with Asian cohosh (Cimicifuga) without removing the fibrous roots. The safety of Cimicifuga rhizome and fibrous roots is unknown and has not been reported. Therefore, in this paper, the rhizome and fibrous roots of Cimicifuga dahurica (Turcz.) Maxim (C. dahurica) were completely separated, extracted with 70% ethanol, and freeze-dried to obtain crude rhizome extract (RC) and fibrous roots extract (FRC). UHPLC-Q-TOF-MS was used to identify 39 compounds in the rhizome and fibrous roots of Cimicifuga, mainly saponins and phenolic acids. In the L-02 cytotoxicity experiment, the IC50 of fibrous roots (1.26 mg/mL) was slightly lower than that of rhizomes (1.417 mg/mL). In the 90-day sub-chronic toxicity study, the FRC group significantly increased the level of white blood cells, ALP, ALT, AST, BILI and CHOL (p < 0.05); large area of granular degeneration and balloon degeneration occurred in liver tissue; and the expression of p-NF-kB in the nucleus increased in a dose-dependent manner. Overall, Fibrous roots of Cimicifuga are at risk of hepatotoxicity and should be strictly controlled and removed during the processing.

show abstract

“…The poly (I:C)-induced murine pulmonary inflammation model was established according to the method in our published research ( Hu et al, 2021 ). Briefly, mice were anesthetized by i. p. injection of Somnopentyl ® (Kyoritsu Seiyaku Corp., Tokyo, Japan) or a mixture of three kinds of anesthetics [Domitor ® (Nippon Zenyaku Kogyo, Kōriyama, Japan), Dormicum ® (Maruishi Pharmaceutical, Osaka, Japan), Vetorphale ® (Meiji Animal Health, Kumamoto, Japan)] after drug administration and were inoculated with 25 μl saline solution of poly (I:C) (1 mg/ml) via the right nostril; the procedures were conducted for three consecutive days.…”

Section: Methodsmentioning

confidence: 99%

Integrating network pharmacology and experimental verification to decipher the immunomodulatory effect of Bu-Zhong-Yi-Qi-Tang against poly (I:C)-induced pulmonary inflammation

Yamamoto

Chen

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

Pulmonary inflammation caused by respiratory tract viral infections is usually associated with acute exacerbation of respiratory diseases, such as asthma and chronic obstructive pulmonary disease (COPD). Therefore, maintaining the pulmonary immune homeostasis is particular important for prevention of the acute exacerbation. Bu-Zhong-Yi-Qi-Tang (BZYQT), a traditional Chinese medicine formula, has been broadly used to improve respiratory and gastrointestinal disorders in China for over 700 years. Previously, we have found the regulatory activity of BZYQT on the lower respiratory immune system, while its potential effects during pulmonary inflammation remain unknown. Thus, the current study focused on deciphering its immunomodulatory effect and potential mechanism against pulmonary inflammation by using a viral RNA analogue, poly (I:C), induced murine pulmonary inflammation model and BEAS-2B cell model coupled with network pharmacology. Inflammatory cells in the bronchoalveolar lavage fluid were counted through microscope examination according to the cell’s morphology and staining characteristics; protein and gene levels of inflammatory mediators were determined with Elisa and quantitative PCR, respectively; network pharmacology was conducted based on 46 BZYQT-related potential bioactive components, pulmonary inflammation and immune-related targets. Our results indicated that the recruitment of neutrophils and the expression of Adgre1 (encoding the F4/80, which is a macrophage marker) in the lung induced by poly (I:C) were significantly reduced after BZYQT treatment, and these effects were further demonstrated to be related to the interference of leukocyte transendothelial migration from the decreased levels of CXCL10, IL-6, TNF-α, CXCL2, ICAM-1, VCAM-1, and E/P-selectins. Furthermore, BZYQT inhibited the CXCL10, TNF-α, and IFN-β expression of poly (I:C)-challenged BEAS-2B cells in a dose-dependent manner. Through integrating results from network pharmacology, experiments, and the published literature, isoliquiritigenin, Z-ligustilide, atractylenolide I, atractylenolide III, formononetin, ferulic acid, hesperidin, and cimigenoside were presumed as the bioactive components of BZYQT against pulmonary inflammation. Overall, our findings demonstrated that BZYQT possesses a pronounced immunomodulatory effect on poly (I:C)-induced pulmonary inflammation, which provides a pharmacological basis for BZYQT in the treatment of respiratory disorders.

show abstract

Chemical profile of Cimicifuga heracleifolia Kom. And immunomodulatory effect of its representative bioavailable component, cimigenoside on Poly(I:C)-induced airway inflammation

Cited by 18 publications

References 24 publications

A multidimensional strategy to rapidly identify the chemical constituents in Shengxian Decoction by using ultra‐performance liquid chromatography coupled with ion mobility spectrometry quadrupole time‐of‐flight mass spectrometry

A multidimensional strategy to rapidly identify the chemical constituents in Shengxian Decoction by using ultra‐performance liquid chromatography coupled with ion mobility spectrometry quadrupole time‐of‐flight mass spectrometry

Fibrous Roots of Cimicifuga Are at Risk of Hepatotoxicity

Integrating network pharmacology and experimental verification to decipher the immunomodulatory effect of Bu-Zhong-Yi-Qi-Tang against poly (I:C)-induced pulmonary inflammation

Contact Info

Product

Resources

About