Chemical profile, antimicrobial potential, and antiaggregant activity of supercritical fluid extract from Agaricus bisporus

Weber, Simone Schneider; Souza, Alessandra Carla Sampaio de; Soares, Denise Caroline Luiz; Lima, Caroline Carvalho; Moraes, Ana Carolina Rabello de; Gkionis, Stephanie Viegas; Arenhart, Tanara; Rodrigues, Luiz Gustavo Gonçalves; Ferreira, Sandra Regina Salvador; Pedrosa, Rozangela Curi; Silva, Denise Brentan; Paredes‐Gamero, Edgar Julian; Perdomo, Renata Trentin; Parisotto, Eduardo Benedetti

doi:10.1007/s11696-022-02308-8

Cited by 3 publications

(2 citation statements)

References 51 publications

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“…Platelet aggregation was assessed by turbidimetry using a semi-automatic aggregometer (EasyAgreg 4.0, Qualiterm ® , São Paulo, Brazil) [21]. Platelets were counted in automatic counting equipment (Sysmex XP-300, Sysmex, Kobe, Japan) and adjusted with saline solution, obtaining a value between 200,000 and 250,000 platelets/mm 3 .…”

Section: Determination Of Platelet Aggregationmentioning

confidence: 99%

“…About 100 µL of PPP was pre-incubated with 1.3 µL of AAPO at different concentrations (50-1000 µg/mL) or controls for 5 min at 37 • C. For normal (standard) and positive controls, plasma without added vehicle and/or treatment and heparin (17 IU/mL blood) were used, respectively. DMSO (0.6%) was used as a negative control [21].…”

Section: Evaluation Of Blood Coagulationmentioning

confidence: 99%

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Antithrombotic Effect of Oil from the Pulp of Bocaiúva—Acrocomia aculeata (Jacq.) Lodd. ex Mart. (Arecaceae)

Espinoça,

Basilio,

de Araujo

et al. 2024

Nutrients

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The study aimed to evaluate the antithrombotic action of Acrocomia aculeata pulp oil (AAPO) in natura, in an in vitro experimental model. AAPO was obtained by solvent extraction, and its chemical characterization was performed by gas chromatography coupled to a mass spectrometer (GC-MS). In vitro toxicity was evaluated with the Trypan Blue exclusion test and in vivo by the Galleria mellonella model. ADP/epinephrine-induced platelet aggregation after treatment with AAPO (50, 100, 200, 400, and 800 μg/mL) was evaluated by turbidimetry, and coagulation was determined by prothrombin activity time (PT) and activated partial thromboplastin time (aPTT). Platelet activation was measured by expression of P-selectin on the platelet surface by flow cytometry and intraplatelet content of reactive oxygen species (ROS) by fluorimetry. The results showed that AAPO has as major components such as oleic acid, palmitic acid, lauric acid, caprylic acid, and squalene. AAPO showed no toxicity in vitro or in vivo. Platelet aggregation decreased against agonists using treatment with different concentrations of AAPO. Oil did not interfere in PT and aPTT. Moreover, it expressively decreased ROS-induced platelet activation and P-selectin expression. Therefore, AAPO showed antiplatelet action since it decreased platelet activation verified by the decrease in P-selectin expression as well as in ROS production.

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Section: Determination Of Platelet Aggregationmentioning

confidence: 99%

Section: Evaluation Of Blood Coagulationmentioning

confidence: 99%

Antithrombotic Effect of Oil from the Pulp of Bocaiúva—Acrocomia aculeata (Jacq.) Lodd. ex Mart. (Arecaceae)

Espinoça,

Basilio,

de Araujo

et al. 2024

Nutrients

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Chemical profile and antithrombotic activity of the essential oil of Levisticum officinale W.D.J.Koch (Apiaceae)

Basilio,

Espinoça,

Coronel

et al. 2024

Chem. Pap.

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Rapid Discovery of Substances with Anticancer Potential from Marine Fungi Based on a One Strain–Many Compounds Strategy and UPLC-QTOF-MS

Wu,

Zhao,

Zhang

et al. 2023

Marine Drugs

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The secondary metabolites of marine fungi with rich chemical diversity and biological activity are an important and exciting target for natural product research. This study aimed to investigate the fungal community in Quanzhou Bay, Fujian, and identified 28 strains of marine fungi. A total of 28 strains of marine fungi were screened for small-scale fermentation by the OSMAC (One Strain-Many Compounds) strategy, and 77 EtOAc crude extracts were obtained and assayed for cancer cell inhibition rate. A total of six strains of marine fungi (P-WZ-2, P-WZ-3-2, P-WZ-4, P-WZ-5, P56, and P341) with significant changes in cancer cell inhibition induced by the OSMAC strategy were analysed by UPLC-QTOF-MS. The ACD/MS Structure ID Suite software was used to predict the possible structures with inhibitory effects on cancer cells. A total of 23 compounds were identified, of which 10 compounds have been reported to have potential anticancer activity or cytotoxicity. In this study, the OSMAC strategy was combined with an untargeted metabolomics approach based on UPLC-QTOF-MS to efficiently analyse the effect of changes in culture conditions on anticancer potentials and to rapidly find active substances that inhibit cancer cell growth.

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Chemical profile, antimicrobial potential, and antiaggregant activity of supercritical fluid extract from Agaricus bisporus

Cited by 3 publications

References 51 publications

Antithrombotic Effect of Oil from the Pulp of Bocaiúva—Acrocomia aculeata (Jacq.) Lodd. ex Mart. (Arecaceae)

Antithrombotic Effect of Oil from the Pulp of Bocaiúva—Acrocomia aculeata (Jacq.) Lodd. ex Mart. (Arecaceae)

Chemical profile and antithrombotic activity of the essential oil of Levisticum officinale W.D.J.Koch (Apiaceae)

Rapid Discovery of Substances with Anticancer Potential from Marine Fungi Based on a One Strain–Many Compounds Strategy and UPLC-QTOF-MS

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