Chemical priming of plant defense responses to pathogen attacks

Hönig, Martin; Roeber, Venja M.; Schmülling, Thomas; Cortleven, Anne

doi:10.3389/fpls.2023.1146577

Cited by 25 publications

(10 citation statements)

References 166 publications

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“…Systemic acquired resistance occurs in disposal plant parts following infection by the pathogen in the localized core of infection. This process is controlled by a signaling pathway depending upon the regulatory protein NPR1 and salicylic acid accumulation (Hönig et al, 2023). The enhanced WRKY6 induction is consistent with enhancement of our related marker genes R1, PR5, and RGA29, basically resistant to FOC for comparing and con rming the results of our research (Chand et The resistant onion ''Saba'' and ''Saba' -HS' showed the highest accumulation of PR5 transcripts at all the course times in our study.…”

Section: Discussionsupporting

confidence: 90%

“…The alarming increase in the human population, and the ever-increasing crop demand led to intensive agricultural operations, which enhance the pathogen attack intensity (Soheili et al, 2023; Gholamaliyan et al, 2021). Chemical method of disease control via chemical pesticides usually affects adversely in nutrient cycles, natural bio-control factors, and bene cial organisms (Haghani et al, 2014;Kasem et al, 2019;Hönig et al, 2023). Thus, the preferred choice is the establishment of resistance in the target crops via genetic engineering technology (Forghani et al, 2021;Filyushin et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Poursakhi,

Asadi-Gharneh,

Esfahani

et al. 2024

Preprint

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Fusarium basal rot (FBR) is a serious disease of onion that causes significant losses globally. There are several control measures, of which planting of resistant FBR-cultivars is the most advantageous control option. Thus, to elucidate the involving mechanisms to FBR infection, we consequently carried out quantification changes in antioxidant enzymes activity including ROS (reactive oxygen species); superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT) in two resistant onion genotypes ‘Saba’ and ‘Saba – HS’; and susceptible ‘Savannah Sweet’ and ‘Golden eye’ following inoculation with FBR agent. As a complimentary to the related antioxidant enzymes, we also characterized the critical transcription factors (TFs); R1, R5 and RGA29 marker genes using qRT-PCR involved in endowing resistance at three time course/week post inoculation (wpi). Additionally, we analyzed the changes in biomass growth parameters (BGPs) accordingly. The results showed that the lowest disease severity percentage (DSP) was in resistant ‘Saba’ and ‘Saba – HS’ (6.7 and 4.7 %), and the highest ones in susceptible ‘Savannah Sweet’ and ‘Golden eye’ (88.9 and 89.6%), respectively. There was also a significant increase in CAT, POX, and SOD activities in resistant genotypes. The highest CAT activity was recorded in resistant ‘Saba’- 4.91, and Saba-HS - 3.75 fold, followed by the same trend relatively for POX (‘Saba’- 3.53 and ‘Saba-HS’- 2.35 fold); and SOD (‘Saba’ 14.46 -fold and ‘Saba-HS’ 12.95 –fold) significantly. It was also found that FOC could up-regulate the defense marker genes; R1, PR5, and RGA29 expression in resistant '‘Saba’' and ‘Saba’-HS ranging from 1.23 to 5.01-fold. The highest regulation was in PR5-5.01 fold followed by R1-4.78; and RGA29-3.83-fold, respectively. In addition, BGPs displayed almost similar trends as was in enzyme and gene expression accordingly. These findings lead to introduction of newly FBR-resistant onion genotypes, and also can be implicated in integrated pest management planning programs against FBR disease. Cumulatively, the proteomic-transcriptomic-data provide novel-insights for improving onion-breeding programs against FBR disease.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Poursakhi,

Asadi-Gharneh,

Esfahani

et al. 2024

Preprint

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“…In this present study, 21-day old leaves or roots were mechanically wounded and immediately subjected to treatment or mock. Since TaCAD-A1 and TaCAD-D1 were detectable without vacuum in ltration or untreated overnight incubation, we postulate that the experimental design of the RNAseq PAMP assay may have skewed the resulting data by priming the tested tissue to produce a stress response (91,92). The differences between the RNAseq and the qRT-PCR assays may also be explained by the high sequence homology present among the class I TaCAD homoeolog cDNA sequences (Supplementary Table S7).…”

Section: Differential Expression Of Class I Tacads May Contribute To ...mentioning

confidence: 99%

Genome-wide characterization and expression analysis of the CINNAMYL ALCOHOL DEHYDROGENASE gene family in Triticum aestivum

Peracchi,

Brew-Appiah,

Garland-Campbell

et al. 2024

Preprint

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Background CINNAMYL ALCOHOL DEHYDROGENASE (CAD) catalyzes the NADPH-dependent reduction of cinnamaldehydes into cinnamyl alcohols and is a key enzyme found at the final step of the phenylpropanoid pathway. Cinnamyl alcohols and their conjugates are subsequently polymerized in the secondary cell wall to form lignin. CAD genes are typically encoded by multi-gene families and thus traditionally organized into general classifications of functional relevance. Results In silico analysis of the hexaploid Triticum aestivum genome revealed 47 high confidence TaCAD copies, of which three were determined to be the most significant isoforms (class I) considered bone fide CADs. Class I CADs were expressed throughout development both in RNAseq data sets as well as via qRT-PCR analysis. In addition, Class I TaCADs were also upregulated after wounding and chitin elicitation in RNAseq data sets, but not in qRT-PCR experiments in roots or shoots. Of the 37 class II TaCADs identified, two groups were observed to be significantly co-expressed with class I TaCADs in developing tissue and under chitin elicitation in RNAseq data sets. These co-expressed class II TaCADs were also found to be phylogenetically unrelated to a separate clade of class II TaCADs previously reported to be an influential resistance factor to pathogenic fungal infection. Lastly, two groups were phylogenetically identified as class III TaCADs, which possess distinct conserved gene structures. However, the lack of data supporting their catalytic activity for cinnamaldehydes and their bereft transcriptional presence in lignifying tissues challenges their designation and function as CADs. Conclusions Taken together, the TaCAD gene family contributes overlapping but nonredundant functions that likely contribute to T. aestivum growth across a wide variety of agroecosystems and tolerance to a large variety of stressors.

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“… 1 , 2 A diverse set of stimuli has been shown for being effective in priming the plant immune system against pathogens. 3 , 4 This includes abiotic changes and pretreatments with altered environmental conditions as a consequence of activated cross-tolerance. Several short (1.5 h) and repetitive cold (4°C) or heat (38°C) treatments increase the resistance of Arabidopsis thaliana (Arabidopsis) against the hemi-biotrophic, virulent pathogen Pseudomonas syringae pv.…”

Section: Short Communicationmentioning

confidence: 99%

Cold priming on pathogen susceptibility in the Arabidopsis eds1 mutant background requires a functional stromal Ascorbate Peroxidase

Schütte,

Baier,

Griebel

2024

Plant Signaling & Behavior

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24 h cold exposure (4°C) is sufficient to reduce pathogen susceptibility in Arabidopsis thaliana against the virulent Pseudomonas syringae pv. tomato ( Pst ) strain even when the infection occurs five days later. This priming effect is independent of the immune regulator Enhanced Disease Susceptibility 1 (EDS1) and can be observed in the immune-compromised eds1–2 null mutant. In contrast, cold priming-reduced Pst susceptibility is strongly impaired in knock-out lines of the stromal and thylakoid ascorbate peroxidases (sAPX/tAPX) highlighting their relevance for abiotic stress-related increased immune resilience. Here, we extended our analysis by generating an eds1 sapx double mutant. eds1 sapx showed eds1 -like resistance and susceptibility phenotypes against Pst strains containing the effectors avrRPM1 and avrRPS4. In comparison to eds1–2 , susceptibility against the wildtype Pst strain was constitutively enhanced in eds1 sapx . Although a prior cold priming exposure resulted in reduced Pst titers in eds1–2 , it did not alter Pst resistance in eds1 sapx . This demonstrates that the genetic sAPX requirement for cold priming of basal plant immunity applies also to an eds1 null mutant background.

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Chemical priming of plant defense responses to pathogen attacks

Cited by 25 publications

References 166 publications

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Genome-wide characterization and expression analysis of the CINNAMYL ALCOHOL DEHYDROGENASE gene family in Triticum aestivum

Cold priming on pathogen susceptibility in the Arabidopsis eds1 mutant background requires a functional stromal Ascorbate Peroxidase

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