“…18) Sterilized BCP liquid medium (1 L; glucose 1.0, yeast extract 2.5, peptone 5.0, polysorbate 80 1.0, L-cysteine 0.1, and bromocresol purple 0.06 g/L) was prepared in a 2-L conical flask. Lactic acid bacteria, Lactobacillus casei, isolated from a commercial environmental preservation materials (named Ehime AI), 23) was inoculated into the BCP liquid medium.…”