Chemical Modification Strategies for Synthesis of Protein-Based Hydrogel

Hwang, Deng Fwu; Damodaran, Srinivasan

doi:10.1021/jf9503826

Cited by 70 publications

(82 citation statements)

References 18 publications

(17 reference statements)

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“…EDTAD was then added incrementally over 30 min, mixed for 3 h, dialyzed (8-kDa molecular weight cut-off) at room temperature for 24 h and then lyophilized to form EDTAD-G (EG) ( Table I). 13 G and EG lysyl groups were also modified with mPEGmA and sodium cyanoborohydride (NaCNBH 3 ) (Aldrich) in a respective 1:0.66:0.186 weight ratio. Briefly, a 20 wt % G or EG solution (0.2 g/mL) was created by dissolving G or EG in deionized water at 50°-60°C for 30 min.…”

Section: Gelatin Backbone Modificationsmentioning

confidence: 99%

“…The percent of gelatin lysyl residues modified by EDTAD and/or mPEGmA was quantified using the established trinitrobenzene sulfonic acid (TNBS) spectrophotometric method. 13,[17][18][19] The percent of gelatin lysyl groups modified by the above procedures is shown in Table II.…”

Section: Gelatin Backbone Modificationsmentioning

confidence: 99%

“…12 EDTAD modification imparts a polyanionic character to the gelatin, resulting in unfolding of the gelatin molecule via intramolecular electrostatic repulsion and provides multiple binding sites for water. 13 PEG exhibits desirable biomedical properties such as protein resistance, low immunogenicity, and enhanced biocompatibility. 14,15 The ethylene oxide repeat units of PEG are easily solvated by water, allowing several water molecules to bind.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and physicochemical analysis of interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate

Burmania¹,

Martínez-Díaz²,

Kao³

2003

J Biomedical Materials Res

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The interrelated effects of gelatin modification, content, and poly(ethylene glycol) molecular weight on the melting temperature, surface hydrophilicity, tensile properties, swelling/degradation, and drug-release kinetics of a novel interpenetrating network (IPN) system containing gelatin and poly(ethylene glycol) diacrylate were evaluated. Gelatin content had a large effect on the IPN melting temperature and Delta H. Modifying gelatin with ethylenediaminetetraacetic acid and/or monomethoxy poly(ethylene glycol) monoacetate ester as well as increasing poly(ethylene glycol) diacrylate molecular weight increased the surface hydrophilicity. Increasing the gelatin weight percent increased the IPN elasticity at room temperature. When buffer and elevated temperature were present in the testing environment, the elasticity of all IPNs tested decreased. IPNs showed an enhanced elasticity and strength when compared with glutaraldehyde-fixed gelatin hydrogels. The extent of IPN swelling and degradation was increased by increasing the gelatin content or by modifying gelatin. The time to complete sample degradation was longer for IPNs when compared with gelatin crosslinked with glutaraldehyde. Modifications to the IPN system increased the maximum percent of chlorhexidine digluconate released from the IPNs. The rate of complete drug release was slower from IPNs than from glutaraldehyde-fixed gelatin matrices. A wide range of IPN physicochemical properties was obtained through formulation changes and chemical modifications.

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Section: Gelatin Backbone Modificationsmentioning

confidence: 99%

Section: Gelatin Backbone Modificationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and physicochemical analysis of interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate

Burmania¹,

Martínez-Díaz²,

Kao³

2003

J Biomedical Materials Res

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“…EDTAD was added incrementally over 30 min, mixed for 3 h, dialyzed (8 kDa molecular weight cut off) at rt for 24 h, and then lyophilized to form EDTAD-modified G (EG). [10] G and EG lysyl groups were also modified with mPEGmA and sodium cyanoborohydride (NaCNBH 3 ) (Aldrich) in a respective 1:0.066:0.186 weight ratio. A 20 weight percent G or EG solution (0.2 g Á mL À1 ) was created by dissolving G or EG in deionized water at 50 to 60 8C for 30 min.…”

Section: Gelatin Backbone Modificationsmentioning

confidence: 99%

“…The percent of gelatin lysyl residues modified by EDTAD and/or mPEGmA was quantified using the established trinitrobenzene sulfonic acid spectrophotometric method. [10][11][12] The extent of gelatin modification is shown in Table 1 and the nomenclature and chemical structure of modified gelatin is shown in Table 2.…”

Section: Gelatin Backbone Modificationsmentioning

confidence: 99%

Mechanical and Chemical Analysis of Gelatin‐Based Hydrogel Degradation

Martínez-Díaz

Nelson

Crone

et al. 2003

Macro Chemistry & Physics

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The interrelated effect of environmental pH and temperature, gelatin backbone modification and content on the tensile and degradative property of interpenetrating networks (IPNs) containing gelatin and poly(ethylene glycol) diacrylate (PEGdA) was examined. Either increasing the PEGdA content or modifying the gelatin backbone with PEG‐monoacetate ester and/or polyanions decreased the IPN elasticity at ambient room temperature (rt). Under an aqueous environment of varying pH levels and elevated temperature, the degradation of IPN tensile properties was further accelerated. IPNs showed an enhanced elasticity and strength when compared to glutaraldehyde‐fixed gelatin hydrogels. Under an aqueous condition, IPNs showed a wider range of degradation products than hydrogels cross‐linked with glutaraldehyde, as characterized with gel permeation chromatography. The nature of IPN degradation products was independent of the type of gelatin backbone modification. The presence of loaded drug, chlorohexidine digluconate, which was found to interact with PEG‐monoacetate esters of the modified gelatin backbone, resulted in unique degradation products. The tensile and chemical degradation of IPNs is a complex interrelationship of the environmental condition, time, and material modification.

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High Capacity Functionalized Protein Superabsorbents from an Agricultural Co‐Product: A Cradle‐to‐Cradle Approach

Capezza

Cui

Numata

et al. 2020

Advanced Sustainable Systems

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Synthesis of superabsorbent particles from nontoxic wheat gluten (WG) protein, as an industrial co‐product, is presented. A natural molecular cross‐linker named genipin (a hydrogenated glycoside) is used together with a dianhydride (ethylenediaminetetraacetic EDTAD), to enable the preparation of a material with a network structure capable of swelling up to ≈4000% in water and ≈600% in saline solution. This represents an increase in swelling by over 10 times compared to the already highly absorbing gluten reference material. The carboxylation (using EDTAD) and the cross‐linking of the protein result in a hydrogel with liquid retention capacity as high as 80% of the absorbed water remaining in the WG network on extensive centrifugation, which is higher than that of commercial fossil‐based superabsorbents. The results also show that more polar forms of the reacted genipin are more effectively grafted onto the protein, contributing to the swelling and liquid retention. Microscopy of the materials reveals extensive nanoporosity (300 nm), contributing to rapid capillarity‐driven absorption. The use of proteins from agricultural industries for the fabrication of sustainable protein superabsorbents is herein described as an emerging avenue for the development of the next generation daily‐care products with a minimal environmental impact.

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Chemical Modification Strategies for Synthesis of Protein-Based Hydrogel

Cited by 70 publications

References 18 publications

Synthesis and physicochemical analysis of interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate

Synthesis and physicochemical analysis of interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate

Mechanical and Chemical Analysis of Gelatin‐Based Hydrogel Degradation

High Capacity Functionalized Protein Superabsorbents from an Agricultural Co‐Product: A Cradle‐to‐Cradle Approach

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