Chemical Modification of Chitosan as a Gene Transporter

Jiang, Hu‐Lin; Liu, Xing; Luo, Cheng-Qiong; Zhou, Tian‐Jiao; Li, Huishan; Cho, Chong‐Su

doi:10.2174/1385272821666170926163544

Cited by 10 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although chitosan offers several favourable characteristics as an excellent gene carrier, unmodified chitosan is restricted by its low solubility in physiological conditions. Therefore, chitosan derivatives with enhanced solubility compared to chitosan for gene delivery have been well explored and summarized in several reviews [24,25,26]. For example, Andrey S. et al [14] have explored the covalent modification of chitosan by hydrophilic modification (Scheme 1 in Figure 2) or hydrophobic modification (Scheme 2 in Figure 2).…”

Section: Design Criteria and Requirements For Gene Delivery Using mentioning

confidence: 99%

“…Therefore, it is critical to fine-tune PEGylation degrees to generate chitosan/siRNA nanocomplexes with maximum transfection efficiency. In addition to PEG, some other hydrophilic modifications have also been conjugated to chitosan for gene delivery, such as dextran, poly (vinyl pyrrolidone), poly (β-malic acid) and poly (aspartic acid) [25]. Most of the covalent conjugations are based on the modification of amine groups of chitosan, which may decrease the charge and finally affect the gene binding efficiency.…”

Section: Design Criteria and Requirements For Gene Delivery Using mentioning

confidence: 99%

“…These spermine-SA-chitosan/pDNA nanocomplexes exhibited high DNA binding ability, lysosomal escape property, high gene knock-down and low cytotoxicity [30]. More chemical modification methods of chitosan can be found in reviews done by Hu-lin Jiang et al [25] and Andrey et al [14]. Although several chitosan derivatives have been developed, continued preclinical studies are necessary to fully understand their safety for use and efficiency.…”

Section: Design Criteria and Requirements For Gene Delivery Using mentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances in Chitosan-Based Carriers for Gene Delivery

et al. 2019

View full text Add to dashboard Cite

Approximately 4000 diseases are associated with malfunctioning genes in a particular cell type. Gene-based therapy provides a platform to modify the disease-causing genes expression at the cellular level to treat pathological conditions. However, gene delivery is challenging as these therapeutic genes need to overcome several physiological and intracellular barriers in order, to reach the target cells. Over the years, efforts have been dedicated to develop efficient gene delivery vectors to overcome these systemic barriers. Chitosan, a versatile polysaccharide, is an attractive non-viral vector material for gene delivery mainly due to its cationic nature, biodegradability and biocompatibility. The present review discusses the design factors that are critical for efficient gene delivery/transfection and highlights the recent progress of gene therapy using chitosan-based carriers.

show abstract

Section: Design Criteria and Requirements For Gene Delivery Using mentioning

confidence: 99%

Section: Design Criteria and Requirements For Gene Delivery Using mentioning

confidence: 99%

Section: Design Criteria and Requirements For Gene Delivery Using mentioning

confidence: 99%

See 1 more Smart Citation

Recent Advances in Chitosan-Based Carriers for Gene Delivery

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Chitosan contains slightly positive charge in acidic media which allows the attachment of nucleic acids to the cationic chitosan. The DNA, siRNA and nanoparticles of nucleic acid could therefore be attached to chitosan for genes delivery [58]. However, its poor solubility in water makes it less efficient when compared with other synthetic cationic polymer such as polyethylenimine (PEI) and poly-L-lysine (PLL) [59].…”

Section: Chitosan Encapsulation Of Drugsmentioning

confidence: 99%

Recent developments in chitosan encapsulation of various active ingredients for multifunctional applications

Raza

Khalil

Ayub

et al. 2020

Carbohydrate Research

134

View full text Add to dashboard Cite

Microencapsulation being an emerging technique has provided effective solution to the challenges faced by pharmaceutical, cosmetic, food agriculture and textile industries to deliver ingredients in their active forms to the target sites. Chitosan is a non-toxic, biodegradable and biocompatible amino polysaccharide which makes it useful for the encapsulation of various active ingredients with positive potential applications. Chitosan coating on food products, for example, gives them protection from possible antimicrobial attacks, antioxidants and extended shelf life. Likewise, its coating on pharmaceutics has valuable applications in preservation dn targeted drug delivery. In this review, we discuss the formation of chitosan, its properties, microencapsulation process, micro-capsular morphologies, selection of core and shell materials in addition to the process of chitosan encapsulation of various active ingredients and their applications in various fields of science and technology.

show abstract

“…However, chitosan itself has some limitations, including a low solubility in water at physiological pH and a relatively low transfection efficiency [15]. Fortunately, chemical modification can result in a polymer with more desirable properties [16,17].…”

Section: Introductionmentioning

confidence: 99%

Effect of Double Substitution in Cationic Chitosan Derivatives on DNA Transfection Efficiency

et al. 2020

View full text Add to dashboard Cite

Recently, much effort has been expended on the development of non-viral gene delivery systems based on polyplexes of nucleic acids with various cationic polymers. Natural polysaccharide derivatives are promising carriers due to their low toxicity. In this work, chitosan was chemically modified by a reaction with 4-formyl-n,n,n-trimethylanilinium iodide and pyridoxal hydrochloride and subsequent reduction of the imine bond with NaBH4. This reaction yielded three novel derivatives, n-[4-(n’,n’,n’-trimethylammonium)benzyl]chitosan chloride (TMAB-CS), n-[(3-hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridine)methyl]chitosan chloride (Pyr-CS), and n-[4-(n’,n’,n’’-trimethylammonium)benzyl]-n-[(3-hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridine)methyl]chitosan chloride (PyrTMAB-CS). Their structures and degrees of substitution were established by 1H NMR spectroscopy as DS1 = 0.22 for TMAB-CS, DS2 = 0.28 for Pyr-CS, and DS1 = 0.21, DS2 = 0.22 for PyrTMAB-CS. Dynamic light scattering measurements revealed that the new polymers formed stable polyplexes with plasmid DNA encoding the green fluorescent protein (pEGFP-N3) and that the particles had the smallest size (110–165 nm) when the polymer:DNA mass ratio was higher than 5:1. Transfection experiments carried out in the HEK293 cell line using the polymer:DNA polyplexes demonstrated that Pyr-CS was a rather poor transfection agent at polymer:DNA mass ratios less than 10:1, but it was still more effective than the TMAB-CS and PyrTMAB-CS derivatives that contained a quaternary ammonium group. By contrast, TMAB-CS and PyrTMAB-CS were substantially more effective than Pyr-CS at higher polymer:DNA mass ratios and showed a maximum efficiency at 200:1 (50%–70% transfected cells). Overall, the results show the possibility of combining substituent effects in a single carrier, thereby increasing its efficacy.

show abstract

Chemical Modification of Chitosan as a Gene Transporter

Cited by 10 publications

References 0 publications

Recent Advances in Chitosan-Based Carriers for Gene Delivery

Recent Advances in Chitosan-Based Carriers for Gene Delivery

Recent developments in chitosan encapsulation of various active ingredients for multifunctional applications

Effect of Double Substitution in Cationic Chitosan Derivatives on DNA Transfection Efficiency

Contact Info

Product

Resources

About