Chemical modification of cell death in the Bar eye of Drosophila

Inhibition of RNA Interference and Modulation of Transposable Element Expression by Cell Death in Drosophila

“…When B mutant larvae are raised on food containing 1-2% acetamine, the mutant effect can be largely (but not completely) reversed by prolonging development, which significantly reduces cell death in the late third instar (Fristrom 1972). When female animals with B and GMR-wIR on the X chromosomes were treated with acetamine, reversal of RNAi is diminished as expected ( Figure 1A).…”

FINE STRUCTURAL AND BIOCHEMICAL STUDIES ON CELL DEATH OF THE DEVELOPING <i>BAR</i> EYE DISCS IN <i>DROSOPHILA MELANOGASTER</i>

“…Recent evidences indicated that the cell death appears in the specific regions of the mutant Bar eye discs, i.e., in the anterior portion of the presumptive ommatidial forming regions (Fristrom 1969(Fristrom , 1972Michinomae and Kaji 1973;Michinomae 1974). As pointed out elsewhere, (Michinomae 1974), the characteristic figures in which the activity of acid phosphatase reaction were detected by histochemical procedure, were found in the regions of cell death.…”

Retrotransposon-induced overexpression of a homeobox gene causes defects in eye morphogenesis in Drosophila.

“…The nature and specificity of the mechanism of tominduced mutagenesis raises the question as to the putative involvement in eye morphogenesis of genes affected by the tom element. Dominant phenotypes caused by tom insertion resemble the Bar phenotype in D. melanogaster, suggesting that cell death in the eye imaginal discs of the larvae may be responsible for the adult defect (Fristrom, 1969(Fristrom, , 1972 CAT CTC AGT CAC CAC CAC CAT CCG GCG CTG CAC CAC CAC CCG GTC CTG CAG CAC CAC TAC AGC CTG CAG CAG CAG CAT CAG CAG CAG CAA +264 His Leu Ser His His His His Pro Ala Leu His His His Pro Val Leu Gtn His His Tyr Ser Leu Gtn Gin Gln His Gin Gin Gtn Gln 54 0 CAG CAG CAG CCA CCA GCA CCA CCG GCG GTA GCC ACA ACC ACG GTG GTC AAC ATG TCC GGC AGC ACC ACC ACG GCG GCT AAT CTG AAG CCC +354 Gln Gln Gtn Pro Pro Ala Pro Pro Ala Vat Ala Thr Thr Thr Vat Val Asn Met Ser Gly Ser Thr Thr Thr ALa Ala Asn Leu Lys Pro 84 AAC CGC TCC CGC TTC ATG ATC AAC GAC ATC CTG GCG GGC AGT GCC GCG GCC GCC TTT TAC AAG CAA CAG CAG CAG CAC CAC CAC CAC CAC +444 Asn Arg Ser Arg Phe Met lie Asn Asp lle Leu Ala Gly Ser Ala Ala Ala Ala Phe Tyr Lys Gin Gin Gin Gin His His His His His 114 0 CAC CAG AGC CAC CAC AAC AAC AAC AAT CAC TCT GGT GGC TCG AGT GGC GGC ACG AGC CCC ACC CAC CAC AAC AAC AAC AAC GGC GAG GGA +534 His Gln Ser His His Asn Asn Asn Asn His Ser Gly Gly Ser Ser Gly Gly Thr Ser Pro Thr His His Asn Asn Asn Asn Gly Glu Gly 144 TTC GAG CCA CCG AGT GGA GGA GCT GGA GCA GGA GCA GGA GCA GCT GCC CCA CCA CCA CCG CTG CAC CAC CTC CAC CCG CAA TCG CAT CCC +624 Phe Glu Pro Pro Ser Gly Gly Ata Gly Ala Gly Ala Gly Ata Ata Ata Pro Pro Pro Pro Leu His His Leu His Pro Gln Ser His Pro 174 p CAT CCG CAT CCG CAT CCG CAC TCA CAC CCA CAT CCA CAC GCC CTG GTG CAT CCG CAC GCC AAG CTG GCC GCG GGC GGT GGC GCC GCC AAC +714 His Pro His Pro His Pro His Ser His Pro His Pro His Ala Leu Val His Pro His Ata Lys Leu Ala Ala Gty Gly Gty Ata Ala Asn 204 GGT CTG AAT GTG GCA CAG TAC GCG GCG GCC ATG CAA CAG CAC TAT GCC GCC GCA GCC GCA GCA GCA GCG GCC CGA AAC AGT GCA GCA GCA +804 Gly Leu Asn Vat Ala Gln Tyr Ala Ala ALa Met Gin Gin His Tyr Ala Ala Ala Ala ALa Ata Ata Ala Ata Arg Asn Ser Ala Ala Ala 234 GCC GCC GCG GCA GCC GCA GCA GCA GCA TCA GCC GCC GCC GCC GGA GGA GGA GGA GGA GGT GGC CTG GGT GTG GGT GGT GCG CCG GCG GGA +894 Ala Ata Ata Ala Ala Ata Ala Ala Ata Ser Ata Ala Ata Ala Gty Gty Gly GLY Gly Gty Gly Leu Gly Val Gly Gly Ala Pro Ala Gty 264 GCG GAG CTG GAC GAC AGC AGC GAT TAT CAC GAG GAG AAC GAG GAC TGC GAC AGC GAC GAG GGC GGC AGC GCG GGA GGC GGC GGG GGA GGC +984 Ala Gtu Leu Asp Asp Ser Ser Asp Tyr His Glu Glu Asn Gtu Asp Cys Asp Ser Asp Glu Gly Gly Ser Ala GtY Gly Gly Gty Gly Gly 294 AGC AAC CAC ATG GAC GAT CAC AGC GTT TGT AGC AAT Ggtgaggtg--(2421 bp)--cttttagGC GGC AAG GAC GAT GAC GGA AAC AGC ATT AAG +3489 Ser Asn His Met Asp Asp His Ser Vat Cys Ser Asn G ly Gty Lys Asp Asp Asp Gly Asn Ser lle Lys 317 AGC GGC TCC ACC AGC GAC ATG AGC GGG CTG AGC AAG AAG CAG AGG AAG GCC AGG ACC GCC TTC ACC GAC CAC CAG CTG CAG ACG CTG GAG +3579 Ser Gly Ser Thr Ser Asp Met Ser Gly Leu Ser Lys Lys Gtn Arg LS Ala Ars Thr Ala Phe Thr ASD His Gln Leu Gtn Thr Leu Glu 347 AAG TCC TTC GAG CGG CAG AAG TAC CTC AGT GTC CAG GAG CGC CAG GAG CTG GCC CAC AAG CTG GAC TTG AGC GAC TGC CAG GTG AAG ACC +3669 Lys Ser Phe Gtu Arg GlIn LYS 1ar Leu Ser Vat Gin Glu Arg Gtn Glu Leu Ala His Lys Leu ASP Leu Ser ASP CYS Gin Val LYS Thr 377 TGG TAC CAG AAC AGG AGgtgcgtct--(104 bp)--ctttttagG ACC AAA TGG ATG CGA CAG ACT GCT GTC GGG CTG GAA CTC CTC GCC GAG GCC +3858 Tr2 Tyr Gln Asn I=sAr g Thr Lys Trp) Met Arg, Gin Thr Ala. Val Gty Leu Glu Leu Leu Ala Gtu Ala 400 GGA AAC TTC GCC GCC TTC CAG CGG CTC TAC GGC GGA TCC CCC TAC TTG GGC GCC TGG CCG TAC GCG GCT GCT GCC GGT GCT GGG GCC GCT +3948 Gly Asn Phe Ala Ala Phe Gln Arg Leu Tyr Gly Gty Ser Pro Tyr Leu Gly Ala Trp Pro Tyr Ala Ala Ala Ala Gly Ala Gty Ala Ala 430…”