“…Besides, covalent bonds are formed in derivatization [35] and, therefore, derivatization is a more permanent modification than complexation. The most commonly used derivatizing reagents are diethyldithiocarbamate (DDTC), NTA, EDTA, CDTA, DTPA, tartrate, oxalate, citrate, amino acids and CDs [9]. A good complexing agent must be (i) highly pure, (ii) soluble in BGE, (iii) should form stable, sensitive and a single state complex and (iv) should have good UV absorbance.…”