Chemical modification for improving catalytic performance of lipase B from Candida antarctica with hydrophobic proline ionic liquid

Zhang, Xiaoguang; Xue, Yu; Lu, Zeping; Xu, Huajin; Hu, Yi

doi:10.1007/s00449-022-02696-x

Cited by 9 publications

(1 citation statement)

References 43 publications

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“…suggested that the activity of anhydride modified Novozym‐435 can only be improved by approximately 20%, which is far from the requirements for industrial applications. Most recently, functional ionic liquids (ILs) that were generally used as the reaction medium were confirmed to also act as a promising alternative to traditional chemical modifiers as a result of their advantages with respect to designability, stability and low‐cost 16‐19 . However, the mechanism by which the ILs modifiers enhanced the stability and activity of lipase was still ambiguous.…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid modification reshapes the substrate pockets of lipase to boost its stability and activity in vitamin E succinate synthesis

Ma,

Zhang,

Chen

et al. 2023

J Sci Food Agric

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BACKGROUNDThe relative low stability, reusability and activity of enzymes made the industrial production of vitamin E succinate (VES) can only be performed with complex processes and high cost using chemical methods. To address these issues, in the present study, an ionic liquids (ILs) modification strategy was developed to improve the activity and stability of lipases in VES synthesis.RESULTSThe results showed that the [1‐butyl‐3‐methyl imidazole] [N‐acetyl‐l‐proline] ILs modified Candida rugosa lipase (CRL) has the highest modification degree (48.28%), activity (774 U g−1), thermostability and solvent tolerance in three selected modifiers. Additionally, after reaction condition optimization, the highest yield of VES can be improved to 95.18% at 45 °C for 15 h, which was significantly improved compared to some previous studies.CONCLUSIONIn the present study, a high‐efficiency VES synthesis strategy was successfully developed via modification of lipase. Moreover, the mechanism by which ILs modification can enhance the activity and stability of lipase was investigated via both experimental and computational‐aided methods. Molecular dynamics simulation suggested that ILs modification changed the geometry of Phe344 from flat to upright, which significantly reshaped and enhanced the size of substrate binding pocket of CRL. It is also agreement with our circular dichroism and fluorescence spectroscopy results, which suggested that the modification changed the secondary structure of CRL to a certain extent. The larger pocket also endowed the suitable binding pose of succinate, which made the hydrogen bonds between succinate and active site Ser209 become stronger, and thus improving the yield of VES. © 2023 Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Ionic liquid modification reshapes the substrate pockets of lipase to boost its stability and activity in vitamin E succinate synthesis

Ma,

Zhang,

Chen

et al. 2023

J Sci Food Agric

Self Cite

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Hydrophobic Ionic Liquid Gel Microspheres as Bi‐Component Carriers with a Liquid Phase to Immobilize Enzymes for Enhanced Performance

Ben,

Zhao,

et al. 2024

Adv Funct Materials

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This study focuses on incorporating liquid molecules, different from the bulk solution, into the immobilized enzyme carrier to regulate the distribution effect and diffusion‐limiting impact of the carrier's microenvironment for substrates, which generally is difficult to achieve due to the instability of the materials with liquid inclusions. A freestanding liquid‐holding particle carrier, with bi‐component hydrophobic ionic liquid gel microspheres with poly (glycidyl methacrylate) as the network and 1‐butyl‐3‐methylimidazolium hexafluorophosphate as the dispersing medium, is proposed, which is stable in air aqueous solution, and can extract proteins and organic small molecules into its interior due to the mobility of its dispersing medium. Horseradish peroxidase is covalently immobilized into the microspheres, forming a liquid compartment enzyme microreactor. The microreactor exhibits superior stability, enzymatic activity, and catalytic performance for Basic Orange II degradation compared to free enzyme and liquid‐free immobilized enzymes. This is attributed to the biocompatibility of the ionic liquid, its role in substrate enrichment in its interior, and its rapid mass transfer capability. This contribution shows the effectiveness of regulating the carrier's microenvironment with liquid molecules, offering fresh perspectives and strategies for enzyme technology.

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A Practical Synthesis of 2‐Arylimino‐2H‐Chromenes via Biocatalytic System in Water

et al. 2023

ChemistrySelect

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A metal‐free, straightforward, non‐hazardous reagent‐added, one‐pot approach for the efficient synthesis of 2‐arylimino‐2H‐chromenes with high yields was developed for the first time in this report through the coupling of salicylaldehydes with β‐ketothioamides, which was catalyzed by lipase in water. This green strategy provides an efficient method to obtain various 2‐arylimino‐2H‐chromenes and enriches the practical application of biocatalysis.

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Chemical modification for improving catalytic performance of lipase B from Candida antarctica with hydrophobic proline ionic liquid

Cited by 9 publications

References 43 publications

Ionic liquid modification reshapes the substrate pockets of lipase to boost its stability and activity in vitamin E succinate synthesis

Ionic liquid modification reshapes the substrate pockets of lipase to boost its stability and activity in vitamin E succinate synthesis

Hydrophobic Ionic Liquid Gel Microspheres as Bi‐Component Carriers with a Liquid Phase to Immobilize Enzymes for Enhanced Performance

A Practical Synthesis of 2‐Arylimino‐2H‐Chromenes via Biocatalytic System in Water

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