Chemical Investigation of a Co-Culture of Aspergillus fumigatus D and Fusarium oxysporum R1

Yu, Ronglu; Li, Mingzhu; Wang, Yi; X, Bai; Chen, Jianwei; Li, Xing‐Nuo; Wang, Hong; Zhang, Huawei

doi:10.25135/rnp.199.20.07.1728

Cited by 9 publications

(7 citation statements)

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“…Studies have pointed out that Fusarium could decompose cellulose and other organic compounds and could degrade phenolic compounds and polycyclic aromatic hydrocarbons at the same time ( Leite et al, 2008 ). Aspergillus could decompose proteins to produce volatile flavors and also produce organic acids ( Yu et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…In short, the core bacterial community in the F-I-D was the most important driving force for the maturation of Daqu . Wheat might be the source of these fungi whose growth was inhibited during the Daqu making ( Yu et al, 2021 ). Another important reason was that these fungi in the environment transferred to the outer of Daqu and enriched in the maturation process and reproduced during maturation under suitable moisture and temperature, and finally increased its abundance in mature Daqu ( Du et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Understanding the Shifts of Microbial Community and Metabolite Profile From Wheat to Mature Daqu

Zhang

Shen²,

Wei³

et al. 2021

Front. Microbiol.

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Wheat-originated microbes play an important role in shaping the quality of high-temperature Daqu which is commonly used as a starter for producing sauce-flavor Baijiu. However, the shifts of microbiota from raw material to fresh Daqu and then to mature Daqu remain unclear. Hence, in the present study, the inner and outer of fresh and mature Daqu were collected to explore the correlation between microbiota and metabolites as well as the source of the microbiota in Daqu. Results indicated that the activities of amylase and protease between the inner and outer of fresh Daqu varied significantly while both parts became similar after maturation. The predominant bacteria shifted from Saccharopolyspora (outer) and Staphylococcus (inner) to Kroppenstedtia (both outer and inner), while the predominant fungi shifted from Thermoascus (both outer and inner) to Byssochlamys (outer) and Fusarium (inner). A combining analysis of headspace solid-phase micro extraction-gas chromatography-mass spectrometry, headspace gas chromatography-ion mobility spectrometry, and nuclear magnetic resonance was employed to detect the metabolites. The network analysis was conducted to perform the relationships between microbes and metabolites. The results showed that the bacteria, especially Saccharopolyspora, Bacillus, and Acinetobacter, had a strong correlation with the productions of esters, amino acids and their derivatives, and sugars and their derivatives, while most fungi such as Thermoascus, were negatively correlated with the phenylalanine, trimethylamine n-oxide, and isovalerate. SourceTracker analysis indicated that wheat was the important source of the Daqu microbiota, especially, the microorganisms in the inner of Daqu might be the drivers of the microbial succession during maturation. This study provided a comprehensive exploration to understand the microbial sources and shifts in high-temperature Daqu during maturation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Understanding the Shifts of Microbial Community and Metabolite Profile From Wheat to Mature Daqu

Zhang

Shen²,

Wei³

et al. 2021

Front. Microbiol.

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“…and Rumex madaio Makino. The metabolites did not show toxicity against human pathogens [ 35 ]. Finally, 2 was isolated, together with five undescribed α-pyrones named penpolonins A–E, from the endophytic fungus Penicillium polonicum Zalessky collected from the roots of Camptotheca acuminata Decne, showing a moderate cytotoxic activity [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Araufuranone: A New Phytotoxic Tetrasubstituted Dihydrofuro[3,2-b]furan-2(5H)-One Isolated from Ascochyta araujiae

et al. 2022

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Araujia hortorum is a perennial vining plant species native to South America. It was introduced into many countries for ornamental and medicinal purposes as well as for its edible fruits, but it has become highly invasive, generating severe environmental problems. Biological control using bioherbicides and natural compounds is an interesting control option. The pathogenic fungus Ascochyta araujiae, isolated from infected leaves of A. hortorum, could be considered as a potential biocontrol agent. Its ability to produce bioactive metabolites was studied. The organic extract of the fungal culture filtrates showed interesting phytotoxic activities consisting of clearly visible necrotic symptoms (0.5–1 cm in diameter) in the punctured leaves. Thus, it was purified; this afforded three main metabolites. These were chemically and biologically characterised: one proved to be a new pentasubstituted dihydrofuro[3,2-b]furan-2(5H)-one, named araufuranone (1). The others were the already known fungal metabolites neovasinin and 2,4-dihydroxy-6-hydoxymethylbenzaldehyde (2 and 3). The structure of araufuranone was determined using spectroscopic methods (essentially 1D and 2D 1H and 13C NMR and HR ESIMS spectra); its relative configuration was assigned by a NOESY spectrum. To the best of our knowledge, araufuranone is the first example of a naturally occurring compound showing that carbon skeleton. Assayed by a puncture, araufuranone proved to be weakly active on the leaves of Diplotaxis sp. and Sonchus sp.; the other two metabolites were even less toxic. Tested on cress, compounds 2 and 3 were able to partially inhibit rootlet elongation whereas araufuranone was almost inactive.

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“…Cyclosporin C belongs to one class of cyclic depsipeptides and is used as a cyclophilin inhibitor for the prevention or treatment of diseases or disorders, such as organ injury or organ failure [33,34]. Thirteen genes involved in the biosynthesis of cyclosporin C were highly similar (identities and similarities > 80%) with In our previous chemical investigation of strain R1, two novel polyketides (Figure 7a,b) along with eleven known substances (Figure 7c-m) had been isolated and characterized (Figure 7) [39][40][41]. Antimicrobial tests showed that compound 1 had a potent inhibitory effect on Staphyloccocus aureus ATCC 2592 with a MIC value of 6.25 µM and compound 6 displayed weak anti-Helicobacter pylori capability at 16 µM.…”

Section: Analysis Of Secondary Metabolite Biosynthetic Gene Clustersmentioning

confidence: 99%

Genome Features and AntiSMASH Analysis of an Endophytic Strain Fusarium sp. R1

Liu

Tang

et al. 2022

Metabolites

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Endophytic fungi are one of the most prolific sources of functional biomolecules with therapeutic potential. Besides playing an important role in serious plant diseases, Fusarium strains possess the powerful capability to produce a diverse array of bioactive secondary metabolites (SMs). In order to in-depth mine gene clusters for SM biosynthesis of the genus Fusarium, an endophytic strain Fusarium sp. R1 isolated from Rumex madaio Makino was extensively investigated by whole-genome sequencing and in-depth bioinformatic analysis, as well as antiSMASH annotation. The results displayed that strain R1 harbors a total of 51.8 Mb genome, which consists of 542 contigs with an N50 scaffold length of 3.21 Mb and 50.4% GC content. Meanwhile, 19,333 functional protein-coding genes, 338 tRNA and 111 rRNA were comprehensively predicted and highly annotated using various BLAST databases including non-redundant (Nr) protein sequence, nucleotide (Nt) sequence, Swiss-Prot, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG), as well as Pathogen Host Interactions (PHI) and Carbohydrate-Active enzymes (CAZy) databases. Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) results showed that strain R1 has 37 SM biosynthetic gene clusters (BGCs), including 17 nonribosomal peptide synthetases (NRPSs), 13 polyketide synthetases (PKSs), 3 terpene synthases (Ts), 3 hybrid NRPS + PKS and 1 hybrid indole + NRPS. These findings improve our knowledge of the molecular biology of the genus Fusarium and would promote the discovery of new bioactive SMs from strain R1 using gene mining strategies including gene knockout and heteroexpression.

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Chemical Investigation of a Co-Culture of Aspergillus fumigatus D and Fusarium oxysporum R1

Cited by 9 publications

References 0 publications

Understanding the Shifts of Microbial Community and Metabolite Profile From Wheat to Mature Daqu

Understanding the Shifts of Microbial Community and Metabolite Profile From Wheat to Mature Daqu

Araufuranone: A New Phytotoxic Tetrasubstituted Dihydrofuro[3,2-b]furan-2(5H)-One Isolated from Ascochyta araujiae

Genome Features and AntiSMASH Analysis of an Endophytic Strain Fusarium sp. R1

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