Chemical intervention tools to probe phosphoinositide-dependent signalling

Woscholski, Rüdiger

doi:10.1042/bst20140186

Cited by 2 publications

(3 citation statements)

References 36 publications

(40 reference statements)

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“…This subject has been covered extensively in previous reviews, mostly for protein phosphatases. ,,,− Here, we aim to give an overview and briefly summarize previous approaches, mention newer developments, and add considerations for the use of phosphatase modulators. For a general classification of phosphatase modulators, the reader is referred to Table (based on ref ).…”

Section: A Brief Guide To the Classification And Resources Of Phospha...mentioning

confidence: 99%

“…Another example is the development of hydrolysis-resistant pyridoxal 5′-phosphate analogs as inhibitors of the pyridoxal phosphatase chronophin . The principle of nonhydrolizable substrate analogs and bioisosters for phosphatase inhibition has also been applied to PIP-phosphatases such as PTEN, for which also small molecule inhibitors were identified . Nevertheless, inhibitors of lipid phosphatases are still of very limited availability.…”

Section: A Brief Guide To the Classification And Resources Of Phospha...mentioning

confidence: 99%

“…190 The principle of nonhydrolizable substrate analogs and bioisosters for phosphatase inhibition has also been applied to PIP-phosphatases such as PTEN, 131 for which also small molecule inhibitors were identified. 156 Nevertheless, inhibitors of lipid phosphatases are still of very limited availability. Examples are inhibitors for inositol 5-phosphatases, including specific ones for OCRL/ INPP5B that were discovered by diverse screening approaches.…”

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confidence: 99%

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Approaches to Study Phosphatases

2016

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Phosphatases play key roles in normal physiology and diseases. Studying phosphatases has been both essential and challenging, and the application of conventional genetic and biochemical methods has led to crucial but still limited understanding of their mechanisms, substrates, and exclusive functions within highly intricate networks. With the advances in technologies such as cellular imaging and molecular and chemical biology in terms of sensitive tools and methods, the phosphatase field has thrived in the past years and has set new insights for cell signaling studies and for therapeutic development. In this review, we give an overview of the existing interdisciplinary tools for phosphatases, give examples on how they have been applied to increase our understanding of these enzymes, and suggest how they-and other tools yet barely used in the phosphatase field-might be adapted to address future questions and challenges.

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Section: A Brief Guide To the Classification And Resources Of Phospha...mentioning

confidence: 99%

Section: A Brief Guide To the Classification And Resources Of Phospha...mentioning

confidence: 99%

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Approaches to Study Phosphatases

2016

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Phosphoinositides in Control of Membrane Dynamics

Schink

Tan

Stenmark

2016

Annu. Rev. Cell Dev. Biol.

242

268

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Most functions of eukaryotic cells are controlled by cellular membranes, which are not static entities but undergo frequent budding, fission, fusion, and sculpting reactions collectively referred to as membrane dynamics. Consequently, regulation of membrane dynamics is crucial for cellular functions. A key mechanism in such regulation is the reversible recruitment of cytosolic proteins or protein complexes to specific membranes at specific time points. To a large extent this recruitment is orchestrated by phosphorylated derivatives of the membrane lipid phosphatidylinositol, known as phosphoinositides. The seven phosphoinositides found in nature localize to distinct membrane domains and recruit distinct effectors, thereby contributing strongly to the maintenance of membrane identity. Many of the phosphoinositide effectors are proteins that control membrane dynamics, and in this review we discuss the functions of phosphoinositides in membrane dynamics during exocytosis, endocytosis, autophagy, cell division, cell migration, and epithelial cell polarity, with emphasis on protein effectors that are recruited by specific phosphoinositides during these processes.

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Chemical intervention tools to probe phosphoinositide-dependent signalling

Cited by 2 publications

References 36 publications

Approaches to Study Phosphatases

Approaches to Study Phosphatases

Phosphoinositides in Control of Membrane Dynamics

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