Chemical indicators as enhancers of the chemiluminescent luminol-H2O2-horseradish peroxidase reaction

Dı́az, A. Navas; Sánchez, Francisco; García, J.A. González

doi:10.1016/1010-6030(94)03970-6

Cited by 20 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous, certain chemical indicators, such as phenolphtha-lein, cresolphthalein, phenol red, bromophenol blue, etc. have been reported as enhancers of luminol-H 2 O 2 -HRP CL reaction [17][18][19]. In our preliminary experiment, the enhancing effect of iodophenol blue on luminol-H 2 O 2 -HRP CL system was firstly investigated.…”

Section: Introductionmentioning

confidence: 99%

Iodophenol blue-enhanced luminol chemiluminescence and its application to hydrogen peroxide and glucose detection

Wang

Zhao

et al. 2016

Talanta

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Iodophenol blue-enhanced luminol chemiluminescence and its application to hydrogen peroxide and glucose detection

Wang

Zhao

et al. 2016

Talanta

View full text Add to dashboard Cite

“…[44] Certain chemical indicators, such as phenolphthalein, cresolphthalein, phenol red, and so on, have also been developed as enhancers of the HRP-catalyzed chemiluminescent oxidation of luminol. [45] In this work, we present a sensitive and promising analytical method employing a new CL enhancer, bromophenol blue (BPB), based on a an MB separation and AuNP labeling technique. This novel strategy takes advantage of easy magnetic separation by MBs and amplification feature of colloidal gold label along with CL intensity enhanced by BPB for the luminol-H 2 O 2 -HRP system.…”

Section: Introductionmentioning

confidence: 99%

“…[45,48] For the luminol-H 2 O 2 -HRP-BPB system, the enhanced CL spectra was obtained immediately after injection luminol into H 2 O 2 -HRP-BPB substrate solution ( Figure 5). …”

mentioning

confidence: 99%

Gold Nanolabels for New Enhanced Chemiluminescence Immunoassay of Alpha‐Fetoprotein Based on Magnetic Beads

Yan

Yang

et al. 2009

Chemistry A European J

151

View full text Add to dashboard Cite

Gold'n'beads: A chemiluminescence immunoassay for the sensitive and rapid determination of AFP has been developed, employing bromophenol blue as a novel chemiluminescence enhancer by taking advantages of easy separation by magnetic beads and signal amplification by gold nanoparticles based on a sandwich-type immunoreaction (see scheme).A novel and sensitive chemiluminescence immunoassay (CLIA) has been developed by employing a new chemiluminescence (CL) enhancer, bromophenol blue (BPB), for the determination of alpha-fetoprotein (AFP) based on magnetic beads (MBs) and colloidal gold nanoparticles (AuNPs) modified with HRP-labeled anti-AFP antibodies. BPB, as a chemical indicator, was found to act as a novel and highly signal enhancer of the peroxidase-catalyzed CL reaction of luminol with hydrogen peroxide. After optimizing the CL reaction conditions, this new luminol-H(2)O(2)-HRP-BPB CL system was applied to a sandwich-type CLIA based on the magnetic separation and the amplification feature of AuNPs as HRP labels. A linear range was obtained when the concentrations of AFP were from 0.1 to 5.0 ng mL(-1) (R=0.9997) with the detection limit of 0.01 ng mL(-1) (3sigma), which is one order of magnitude lower than that obtained without using AuNPs, and much lower than that typically achieved by ELISA. The present method was successfully applied to the determination of AFP in human serum samples. The results indicated that this proposed protocol could be quite promising for the application in immunoassays.

show abstract

“…A typical CL system is luminol-H 2 O 2 -Horseradish Peroxidase (HRP), in which HRP-catalyzed reaction between luminol and H 2 O 2 produces CL. It is already known that numerous phenol derivatives can enhance luminol-H 2 O 2 -HRP CL (Diaz et al, 1995). In this work, bromophenol blue was used as the enhancer of the chemiluminescent luminol-H 2 O 2 -HRP reaction.…”

Section: Cntsmentioning

confidence: 99%

Detection of Cancer Biomarkers With Nanotechnology

Zhang

et al. 2013

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Early detection of cancer biomarkers with high precision is critically important for cancer therapy. A variety of sensors based on different nanostructured materials have attracted intensive research interest due to their potential for highly sensitive and selective detection of cancer biomarkers. This review covers the use of a variety of nanostructured materials, including carbon nanotubes, silicon nanowires, gold nanoparticles and quantum dots, in the fabrication of sensors. Emphases are placed on how the detection systems work and what detection limits can be achieved. Some assays described in this review outperform established methods for cancer biomarker detection. It is highly promising that these sensors would soon move into commercial-scale production and find routine use in hospitals.

show abstract

Chemical indicators as enhancers of the chemiluminescent luminol-H2O2-horseradish peroxidase reaction

Cited by 20 publications

References 10 publications

Iodophenol blue-enhanced luminol chemiluminescence and its application to hydrogen peroxide and glucose detection

Iodophenol blue-enhanced luminol chemiluminescence and its application to hydrogen peroxide and glucose detection

Gold Nanolabels for New Enhanced Chemiluminescence Immunoassay of Alpha‐Fetoprotein Based on Magnetic Beads

Detection of Cancer Biomarkers With Nanotechnology

Contact Info

Product

Resources

About