Chemical fingerprint, acute oral toxicity and anti-inflammatory activity of the hydroalcoholic extract of leaves from Tocoyena formosa (Cham. & Schlecht.) K. Schum

Abstract

Inflammation is a protective response of the organism against damaging agents, this process is considered beneficial, however in some situations, this response can be damage when exacerbated effect are present. This claim objective to evaluate the qualitative and quantitative chemical profile, acute toxic and anti-inflammatory effects of the hydroalcoholic extract of leaves from Tocoyena formosa (Cham. & Schlecht.) K. Schum . (HELTF). Quantitative and qualitative phytochemical analysis was performed by HPLC-DAD and colorimetric assay. The topical anti-inflammatory activity was determined in Croton oil-induced ear edema assay and systemic activity was performed in vascular permeability, paw edema induced by carrageenan and dextran. Phytochemical analysis of leaves from HELTF showed presence of tannin, flavonoid, saponins an other that confirmed by HPLC analysis. The extract did not cause significant with LD 50 greater than 5000 mg/kg and did not promote significate reduction in topical inflammatory process. However, HELTF demonstrate significant reduction of paw edema induced by carrageenan and dextran. The HELTF (200 mg/kg) reduced the protein/cell migration in the intradermal carrageenan-induced inflammation. Our results demonstrated that the first time the chemical profile and describe the effective action in systemic anti-inflammatory, antiedematogenic activity and low acute toxicity. This activity presents, supporting its traditional use. However, new studies are necessary for the detection and clarification of the possible mechanism of action.

COMPOSIÇÃO QUÍMICA E ATIVIDADE ANTILEISHMANIA DE Tocoyena hispidula

“…[8][9][10] Algumas atividades biológicas têm sido relatadas, como anti-inflamatória, antinociceptiva, antifúngica, estimulante em útero de ratas, antioxidante e gastroprotetora para T. formosa e capacidade de diminuição da perda óssea em ratos com periodontite para T. sellowiana. 9,11 Tocoyena hispidula Standl é conhecida popularmente por flor-do-cerrado/angelca e jenipapinho, sendo encontrada nas regiões Norte (Pará) e Nordeste (Maranhão e Piauí) do Brasil na forma de arbustos ou subarbustos. Tradicionalmente suas raízes são utilizadas no combate a dor de barriga e inflamação do útero, 12 entretanto, não há registros de estudo químico e biológico sobre esta espécie.…”