Chemical, electron microscopic and physiological observations on the role of catecholamines in the carotid body

Zapata, P.; Hess, Arthur; Bliss, Eugene L.; Eyzaguirre, C.

doi:10.1016/0006-8993(69)90123-1

Cited by 131 publications

(60 citation statements)

References 38 publications

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“…However, large doses of reserpine (5-10 mg/kg), which caused a marked decrease in the content of catecholamines in the carotid body (Zapata et al, 1969), may have also reduced the. content of 5-HT as well, since in other tissues, acute reserpine-treatment produces considerable decreases in the content of catecholamines as well as 5-HT and furthermore, the time course of the decrease in the 5-HT content is very similar to that for catecholamines (see Carlsson,1 966).…”

Section: Discussionmentioning

confidence: 99%

“…However, 20 h after a large dose of reserpine, chemoreceptor discharges still occurred, and NaCN as well as asphyxia produced marked chemoreceptor activation (Zapata et al, 1969). Nevertheless, it is possible that 5-HT may play a more subtle role in chemoreceptor mechanisms, since chemoreceptors in the carotid body became more susceptible to 5-HT after reserpine.…”

Section: Discussionmentioning

confidence: 99%

“…Catecholamines and indolamine are known to be normally present in the carotid body of different mammalian species (Lever, Lewis & Boyd, 1959;Niemi & Ojala, 1964;Fillenz & Woods, 1966;Hamberger, Ritzen & Wersall, 1966;Chiocchio, Biscardi & Tramezzani, 1966I-Li Chen, Yates & Duncan, 1967;Zapata, Hess, Bliss & Eyzaguirre, 1969;Kobayashi, 1971;Chiocchio, King, Carballo & Angelakos, 1971 (Eyzaguirre & Koyano, 1965;Eyzaguirre & Zapata, 1968;Zapata et al, 1969), although other evidence indicates otherwise (Biscoe, 1965;Sampson, 1972;Andrew, Black, Comroe & Jacobs, 1972). A physiological role for 5-hydroxytryptamine (5-HT) may not be discarded, since the carotid body contains a certain amount of 5-HT (Chiocchio et al, 1967(Chiocchio et al, , 1971; furthermore, histochemical methods have shown that 5-HT is located in the electron-dense core vesicles of the Type I cells (Chiocchio et al, 1967;I-Li Chen et al, 1967).…”

Section: Introductionmentioning

confidence: 99%

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The Action of 5‐hydroxytryptamine on Chemoreceptor Discharges of the Cats Carotid Body

Nishi

1975

British J Pharmacology

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1 Chemoreceptor discharges were recorded in vivo from fine filaments of the carotid sinus nerve containing a single or several active units; their frequency was used as an index of receptor activity. The effects of 5-hydroxytryptamine (5-HT) on chemoreceptors were studied in 26 adult cats. At times, sinus baroreceptor discharges were recorded from the carotid nerve and the effect of 5-HT on the discharges was examined.2 Intra-carotid injections of 5-HT (2-20 jg) induced a sharp and brief increase in chemoreceptor discharges, followed by depression or block which lasted for several seconds. Repeated injections at short intervals, and a small dose after a large dose of 5-HT resulted in depressed or blocked response to 5-HT. 3 5-HT in high doses (10-20,g, i.a.) slightly depressed the chemoreceptor discharges induced by either acetylcholine (ACh) or NaCN, when these substances were applied within 20 s after 5-HT. 5-HT (5-20,ug, i.a.) applied during asphyxia induced a further increase in chemoreceptor discharges, soon followed by block of the discharges lasting for several seconds. 4 Atropine or hexamethonium in high doses did not change the chemoreceptor response to 5-HT, while that to ACh was markedly depressed. 5 (+)-Lysergic diethylamide (LSD), methysergide or gramine did not alter the response to 5-HT, while LSD in low doses produced a marked increase in chemoreceptor discharges.. 6 Acute and chronic treatment with reserpine (5-10 mg/kg, i.v.) of the animals did not change the sensitivity and the reactivity of the chemoreceptor to ACh and NaCN, while the chemoreceptor response to 5-HT was augmented, indicating an increase in the sensitivity of chemoreceptors to 5-HT. 7 5-HT in small doses (2-10 Mig, i.a.) induced a marked increase in sinus baroreceptor discharges; subsequently discharges were depressed or blocked for several seconds. 8 The results are discussed in relation to possible mechanism of action of 5-HT on the chemoreceptors. It is concluded that the exogenous 5-HT probably acts directly on the chemosensory nerve endings and depolarizes them, but 5-HT contained in the carotid body does not play a significant role in the generation of chemoreceptor discharges.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Action of 5‐hydroxytryptamine on Chemoreceptor Discharges of the Cats Carotid Body

Nishi

1975

British J Pharmacology

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“…On the basis of ultrastructural and fluorescence studies, Hoffman and Bir~ell [18], Blumcke et al [5] and Hervonen et al [17] suggested that hypoxic stimulation of the carotid body depletes the glomus cells of their catecholamines, whereas with similar techniques A1-Lami and Murray [1], Chen et al [8] and Vema [26] could find no evidence for catecholamine depletion, even under conditions of severe anoxia. Using biochemical determinations of endogenous catecholamines, Zapata et al [29] found no change in cat carotid body after severe hypoxia,'-while Mills and Slotkin [ 22] observed a significant decrease in carotid body DA with less severe hypoxia in the same species. More recently, Hellstrom et al [16] also observed a decrease in rat carotid body DA levels with hypoxia.…”

mentioning

confidence: 97%

“…The carotid bodies exposed to low O2 released 81% more [3H]dopamine during this one-hour period than the carotid bodies exposed only to pure oxygen. These data suggest that hypoxia induces release of DA from the carotid body.It is now well documented that the mammalian carotid body contains the biogenic amines dopamine, norepinephrine and 5-hydroxytryptamine [9,12,29], and that of these, dopamine (DA) is present in the tissue in the highest concentration [12,15]. ~ere is also convincing evidence that these substances are contained primarily within the glomus, or Type I, cells of the carotid body [6,7].…”

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confidence: 99%

Increased release of [3H]dopamine during low O2 stimulation of rabbit carotid body in vitro

González

Fidone

1977

Neuroscience Letters

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SUMMARYRabbit carotid bodies synthesized [3H]dopamine (DA) during a 3-h incubation period in modified Tyrode's solution containing 40 uM [3H]tyrosine. Following tbis loading period, the carotid bodies were exposed for one additional hour to unlabelled Tyrode's solution equilibrated with either 10% oxygen in nitrogen or with 100% oxygen. The carotid bodies exposed to low O2 released 81% more [3H]dopamine during this one-hour period than the carotid bodies exposed only to pure oxygen. These data suggest that hypoxia induces release of DA from the carotid body.It is now well documented that the mammalian carotid body contains the biogenic amines dopamine, norepinephrine and 5-hydroxytryptamine [9,12,29], and that of these, dopamine (DA) is present in the tissue in the highest concentration [12,15]. ~ere is also convincing evidence that these substances are contained primarily within the glomus, or Type I, cells of the carotid body [6,7]. Electrophysiological and pharmacological studies in the cat [23,24,28], dog [4] and rabbit (Monti-Bloch and Eyzaguirre, pets. comm.) have shown that exogenous DA alters chemoreceptor activity recorded from the carotid nerve, and it has been suggested that during natural stimulation of the carotid body release of endogenous DA from the glomus cells may function to modulate the activity of chemoreceptor nerve fibers which terminate upon these cells [2,14,28]. However, previous studies of the effects of natural stimulation on the DA stores of the carotid body have produced conflicting results, and there are no ~tudies which have attempted to directly demonstrate either increased release or tunlover of carotid body DA during natttral stimu]a-

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Vesicular nucleotide transporter‐immunoreactive type I cells associated with P2X3‐immunoreactive nerve endings in the rat carotid body

Yokoyama

Yamamoto

Hirakawa

et al. 2019

J of Comparative Neurology

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Chemical, electron microscopic and physiological observations on the role of catecholamines in the carotid body

Cited by 131 publications

References 38 publications

The Action of 5‐hydroxytryptamine on Chemoreceptor Discharges of the Cats Carotid Body

The Action of 5‐hydroxytryptamine on Chemoreceptor Discharges of the Cats Carotid Body

Increased release of [3H]dopamine during low O2 stimulation of rabbit carotid body in vitro

Vesicular nucleotide transporter‐immunoreactive type I cells associated with P2X3‐immunoreactive nerve endings in the rat carotid body

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