Chemical Effects of Tritium Decay in Aqueous Solution of Uracil

Asano, Takeyoshi; Halpern, A.; Stöcklin, G.

doi:10.1524/ract.1983.33.23.75

Cited by 3 publications

(7 citation statements)

References 16 publications

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“…Like in the previous [2-14 C,5-3 H]uracil study [9], only a small fraction of radioactivity was found in the gaseous phase. The yields of tritiated and 14 C-products varied under the influence of oxygen as described in 3.4.…”

Section: Gaseous Productssupporting

confidence: 66%

“…To minimize the detection loss of lower radioactive spots, the TLC spot of [2][3][4][5][6][7][8][9][10][11][12][13][14] 0.9 X 10 s dpm ( 14 C)) was covered with a piece of aluminum foil (> 40 mg/cm 2 ) and was exposed finally without the foil for 10 min.…”

Section: Tlc-spark Chamber Countingmentioning

confidence: 99%

“…The gaseous samples were analyzed by a temperature gradient GC (Intersmat IGC-16) [9]. The GC column was Porapack Q (60-80 mesh) and 300 X 0.4 cm.…”

Section: Temperature Gradient Radio Gc-gas Flow Countingmentioning

confidence: 99%

“…"-radiolysis (R. effects) are operative [8]. More promising are chemical studies in which a technique of double labelling [10] is utilized [6,9]. In this technique, a DNA base contains besides 3 H also 14 C as a marker, so that the end-products can be determined radioanalytically.…”

Section: Introductionmentioning

confidence: 99%

“…Previously we reported on the chemical effects of tritium decay in aqueous solution of uracil [9]. The results proved that whereas both the T. and R. effects contributed to product formation, the products formed by the latter mechanism largely exceeded those formed * Present address: TERUMO Corporation R and D Center, 1500 Inokuchi, Nakaimachi, Ashigarakami-gun, Kanagawa 259-0151,Japan due to the former mechanism (G R > G T ), making a quantitative estimation of the relative importance of both mechanisms difficult.…”

Section: Introductionmentioning

confidence: 99%

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Chemical Effects of Tritium Decay in Aqueous Solutions of Cytosine

Asano¹,

Kiritani²,

Fujita³

et al. 1998

Radiochimica Acta

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The products resulting from the decay of tritium in doubly labelled [2-14 C,5-3 H]cytosine were determined in diluted oxygenated or deaerated aqueous solutions. The effort was to recognize the relative importance of the transmutation effects and internal /?~-radiolysis. To achieve this, experiments were carried out in the presence of a large excess of non-radioactive cytosine (the diluent addition method). Equations were derived to calculate the decomposition of a labelled parent compound as well as the yields of products. The percent decomposition of [2-14 C,5-3 H]-cytosine per Ή decay was 78% in oxygenated solution and 41% in deaerated solution. Various products that resulted from the transmutation effects were observed in both solutions, a major one with the yield of 28% and 22% per Ή decay in oxygenated and deaerated solutions, respectively. It was suggested that a significant amount of a cytosine cation formed by β~ decay revert to stable cytosine by a hydride ion (H ) abstraction.

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Section: Gaseous Productssupporting

confidence: 66%

Section: Tlc-spark Chamber Countingmentioning

confidence: 99%

“…The gaseous samples were analyzed by a temperature gradient GC (Intersmat IGC-16) [9]. The GC column was Porapack Q (60-80 mesh) and 300 X 0.4 cm.…”

Section: Temperature Gradient Radio Gc-gas Flow Countingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chemical Effects of Tritium Decay in Aqueous Solutions of Cytosine

Asano¹,

Kiritani²,

Fujita³

et al. 1998

Radiochimica Acta

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Syntheses of [5‐²H]‐uracil, [5‐²H]‐cytosine, [6‐²H]‐uracil and [6‐²H]‐cytosine

Kiritani

Asano

Fujita

et al. 1986

Labelled Comp Radiopharmac

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SUMMARY 2Facile syntheses of [ 5-2H] -, [ 6-H] -uracil and [ 5-2H7-,[6-HI-cytosine were investigated. The catalytic reaction of uracil or cytosine with 2H2 gas in alkaline media gave rise to [6-HI-compounds almost exclusively. On the other hand, the reaction of 5-bromouracil or 5-bromocytosine with gave rise to a mixture of [5-H]-, 16-HI-and [5-2H,6-2H]-compounds depending on the experimental conditions. By controlling the temperature, the pressure of 2H2 gas and the amounts of catalyst, [5-HI-uracil and [5-HI-cytosine were obtained. The isotopic distribution in each product was measured by 'H NMR spectroscopy combined with an HPLC method.

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Synthesis of [2‐¹⁴C,5‐³H]cytosine and [2‐¹⁴C,5‐³H]uracil via bromination and catalytic bromine‐tritium gas exchange

Asano

Kiritani

1994

Labelled Comp Radiopharmac

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SUMMARYIn micro-scale experiments. 12-'C. 5-SHlcytosine and [2- 'C, 5-3H] uracil were synthesized via bromination and catalytic Br-'H exchange reaction with the use of [2-'4C1-cytosine and -uracil and tritium gas. The double labelling percentages of these groducts were 70 and 26, respectively. It was assumed that 12-' C,S-Br] uracil was subjected to reaction with hydrogen atom originally adsorbed on a palladium catalyst. This is to a31esser extent valid for [2-'"C,5-Br)cytosine. The percentages of H labelling at 5 position of pyrimidine ring of cytosine and uracil were proved to be 96 and 73. respectively. For the analysis and purification of products, the HPLC eluting conditions using C18 reverse column and NaHePO4 aqueous solution or HeO/methanol mixture as eluent were studied. Unreacted tritium gas was recovered with the use of adsorbents such as active charcoal and Zr-V-Fe getter.

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Chemical Effects of Tritium Decay in Aqueous Solution of Uracil

Cited by 3 publications

References 16 publications

Chemical Effects of Tritium Decay in Aqueous Solutions of Cytosine

Chemical Effects of Tritium Decay in Aqueous Solutions of Cytosine

Syntheses of [5‐²H]‐uracil, [5‐²H]‐cytosine, [6‐²H]‐uracil and [6‐²H]‐cytosine

Synthesis of [2‐¹⁴C,5‐³H]cytosine and [2‐¹⁴C,5‐³H]uracil via bromination and catalytic bromine‐tritium gas exchange

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Chemical Effects of Tritium Decay in Aqueous Solution of Uracil

Cited by 3 publications

References 16 publications

Chemical Effects of Tritium Decay in Aqueous Solutions of Cytosine

Chemical Effects of Tritium Decay in Aqueous Solutions of Cytosine

Syntheses of [5‐2H]‐uracil, [5‐2H]‐cytosine, [6‐2H]‐uracil and [6‐2H]‐cytosine

Synthesis of [2‐14C,5‐3H]cytosine and [2‐14C,5‐3H]uracil via bromination and catalytic bromine‐tritium gas exchange

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Syntheses of [5‐²H]‐uracil, [5‐²H]‐cytosine, [6‐²H]‐uracil and [6‐²H]‐cytosine

Synthesis of [2‐¹⁴C,5‐³H]cytosine and [2‐¹⁴C,5‐³H]uracil via bromination and catalytic bromine‐tritium gas exchange