Chemical Derivatization of Metabolite Mass Profiling of the Recretohalophyte Aeluropus lagopoides Revealing Salt Stress Tolerance Mechanism

Abstract

Plants are the primary producers of food for human being. Their intracellular environment alternation is influenced by abiotic stress factors such as drought, heat and soil salinity. Aeluropus lagopoides is a strong halophyte that grows with ease under high saline muddy banks of creeks of Gujarat, India. To study the response of salinity on metabolite changes in Aeluropus, three treatments, i.e. control, salinity and recovery, were selected for both shoot and root tissue. The cytosolic metabolite state was analysed by molecular chemical derivatization gas chromatography mass profiling. During saline treatment, significant increase of compatible solutes in shoot and root tissue was observed as compared to control. Subsequently, metabolic concentration decreased under recovery conditions. The metabolites like amino acids, organic acids and polyols were significantly detected in both shoot and root of Aeluropus under salinity. The metabolites like proline, aspartic acid, glycine, succinic acid and glycolic acid were significantly upregulated under stress. The salicylic acid was found to play a role in maintaining the polyols level by its down-regulation during salinity. The principle component analysis of all detected metabolites in both shoot and root showed that metabolites expressed under salinity (component 1) were highly variable, while metabolites expressed under recovery (component 2) were comparatively less variable as compared to control. The evolved intracellular compartmentalization of amino acids, organic acids and polyols in A. lagopoides can be a hallmark to sustaining at high salinity stress.

Cell Cycle Arrest in Different Cancer Cell Lines (Liver, Breast, and Colon) Induces Apoptosis under the Influence of the Chemical Content of Aeluropus lagopoides Leaf Extracts

“…Aeluropus lagopoides , a halophyte plant from the family Poaceae, is a perennial grass that tolerates salinity and grows in coastal salt marshes [17]. A. lagopoides is a productive plant used as a source of nutrition for livestock [18,19] and, in Arabian countries, for healing wounds and killing pain [20]. To our knowledge, this is the first report on LC-MS/MS profiling and anticancer activity of crude A. lagopoides extracts within cancer cell lines.…”

Metabolomic variability of four macroalgal species of the genus Lobophora using diverse approaches

“…Other markers evidenced by the NMR method include: 2pentenoic, which is a small unsaturated fatty acid previously found in plants (Wu and Chen, 1992). Pentenoic acid was found up-regulated under salinity stress in the halophyte Aeluropus lagopoides (Paidi et al,2017).…”

l-Aspartate: An Essential Metabolite for Plant Growth and Stress Acclimation

“…For example, under alkaline salt stress, a significant increase (3.97-fold) in Asp and other metabolites, such as proline (Pro), Glu, serine (Ser), and alanine (Ala), in wild soybean seedlings compared to semi-wild and cultivated soybean has been observed [85]. In response to 250 mM NaCl salt stress, the level of Asp increased by 11-fold in the root and about 6.2-fold in the shoot of Aeluropus lagopoides [86]. Under the same conditions, Asn, Lys, glycine (Gly), and Pro increased by 1.46-to 9.98-fold in the shoot, while in the root, Gly, Pro, Phe, and ethanolamine increased by approximately 2.5-to 15.6-fold.…”