Chemical derivatization of hydroxyatrazine for gas chromatographic analysis

Determination of terbutryn and its degradation products in water, sediments, aquatic plants, and fish

“…HC has similar partitioning behavior to HT between CH2C12 and water, and since cyprazine is not used extensively, it is unlikely to be encountered in untreated pond samples. Actual recoveries of HT ranged from 40 to 60% by LC analysis which was similar to recoveries of hydroxyatrazine from soils found by GLC (after methylation) (Muir and Baker, 1978; Khan et al, 1975). A liquid chromatogram of a fortified sediment extract containing HC, HT, and DEHT is shown in Figure 3.…”

Metabolism of deethylatrazine, deisopropylatrazine, and hydroxyatrazine by the soluble fraction (105000g) from goose liver homogenates

“…Eluate II was concentrated to about 1 mL on a rotary evaporator at room temperature, and an excess of freshly prepared ethereal solution of diazomethane (prepared from Diazald, Aldrich Co., Inc., Milwaukee, WI) was added until the yellow color persisted (Khan et al, 1975). The flask was stoppered, and the contents were allowed to stand overnight (16 h) at room temperature with occasional shaking.…”

High-pressure liquid chromatographic determination of hydroxy-s-triazine residues in plant material

“…Some reports, which we found in literature, were concerned with the formation of volatile derivatives to allow gas chromatographic determination of hydroxy-s-triazines. Silylation procedures were described first for the derivative formation of hydroxy-s-triazine compounds by Montgomery et al (1968), Flint andAue (1970), and Khan et al (1975). Reaction conditions should be carefully observed, since the reaction can lead to mono-, di-, and trisilyl derivatives.…”

“…Recoveries in corn samples (grain and silage) were reported to be in the range of 62-88% at fortification levels of 0.1-0.2 mg/kg. Khan et al (1975) and Khan and Marriage (1977) described a methylation procedure for hydroxyatrazine with diazomethane. Hydroxyatrazine was converted to atraton with 50-80% yields.…”

“…Alkylation with methyl iodide in strongly basic solutions was used for confirmation of triazine residues by Lawrence (1974) and Cochrane (1975). The same reaction was also successfully used for derivatization of hydroxyatrazine (Khan et al, 1975) with average yields of the trimethylated derivative of 60-80%. Recoveries of fortified soil samples after derivatization by the alkylation method ranged from 53 to 60%.…”