Chemical constituents from the endophytic fungus Trichoderma ovalisporum isolated from Panax notoginseng

Dang, Li-Zhi; Li, Guohong; Yang, Zhongshan; Luo, Shao-Liu; Zheng, Xi; Zhang, Ke‐Qin

doi:10.1007/s13213-010-0043-2

Cited by 27 publications

(13 citation statements)

References 19 publications

(17 reference statements)

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“…However, Dang et al . [34] only reported one fungal isolate ( Trichoderma ovalisporum ) in P. ginseng . This result indicates that distribution of endophytes depends on plant species and geological locations.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, fungi have advantages over plants, such as short cultivation time and large biomass production. However, there are very few reports on fungal endophytes in ginseng [32-34]. Therefore, an extensive investigation of fungal endophytes in ginseng can increase the opportunities of identifying potential fungi that can be used to produce bioactive compounds and control against pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Fungal Endophytes from Three Cultivars of Panax ginseng Meyer Cultivated in Korea

Park¹,

Lim²,

Park³

et al. 2012

Journal of Ginseng Research

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In order to investigate the diversity of endophytes, fungal endophytes in Panax ginseng Meyer cultivated in Korea were isolated and identified using internal transcribed spacer (ITS) sequences of ribosomal DNA. Three cultivars of 3-year-old ginseng roots (Chunpoong, Yunpoong, and Gumpoong) were used to isolate fungal endophytes. Surface sterilized ginseng roots were placed on potato dextrose agar plates supplemented with ampicilin and streptomycin to inhibit bacterial growth. Overall, 38 fungal endophytes were isolated from 12 ginseng roots. According to the sequence analysis of the ITS1-5.8S-ITS2, 38 fungal isolates were classified into 4 different fungal species, which were Phoma radicina, Fusarium oxysporum, Setophoma terrestris and Ascomycota sp. 2-RNK. The most dominant fungal endophyte was P. radicina in 3 cultivars. The percentage of dominant endophytes of P. radicina was 65.8%. The percentage of colonization frequency of P. radicina was 80%, 52.9%, and 75% in Chunpoong, Yunpoong, and Gumpoong, respectively. The second most dominant fungal endophyte was F. oxysporum. The diversity of the fungal endophytes was low and no ginseng cultivar specificity among endophytes was detected in this study. The identified endophytes can be potential fungi for the production of bioactive compounds and control against ginseng pathogens.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fungal Endophytes from Three Cultivars of Panax ginseng Meyer Cultivated in Korea

Park¹,

Lim²,

Park³

et al. 2012

Journal of Ginseng Research

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“…Shikimic acid (197) (Figure 14 ), was obtained from the endophyte Trichoderma ovalisporum strain PRE-5 of the root of the herbal Panax notoginseng. The compound (197) is activity against S. aureus , Bacillus cereus , M. luteus and E. coli (Dang et al, 2010 ).…”

Section: Antibacterials From Endophytic Fungimentioning

confidence: 99%

Endophytic fungi: a reservoir of antibacterials

2015

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Multidrug drug resistant bacteria are becoming increasingly problematic particularly in the under developed countries of the world. The most important microorganisms that have seen a geometric rise in numbers are Methicillin resistant Staphylococcus aureus, Vancomycin resistant Enterococcus faecium, Penicillin resistant Streptococcus pneumonia and multiple drug resistant tubercule bacteria to name a just few. New drug scaffolds are essential to tackle this every increasing problem. These scaffolds can be sourced from nature itself. Endophytic fungi are an important reservoir of therapeutically active compounds. This review attempts to present some data relevant to the problem. New, very specific and effective antibiotics are needed but also at an affordable price! A Herculean task for researchers all over the world! In the Asian subcontinent indigenous therapeutics that has been practiced over the centuries such as Ayurveda have been effective as “handed down data” in family generations. May need a second, third and more “in-depth investigations?”

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“…Endophytic fungi spend all or part of their life cycle in the host consuming nutrients from the intercellular fluids of the plants [10,11]. Some endophytic fungi produce a wide range of bioactive compounds that have commercial potential as important compounds for medicinal, agricultural and industrial purposes [9,12,13], such as alkaloids, steroids, terpenoids, isocoumarin derivatives, quinones, flavonoids, and lignans. These compounds have diverse functions in plants, such as in protection against phytopathogens and the promotion of growth, which occurs due to the production of such phytohormones as auxin and gibberellins and the absorption of such nutrients as nitrogen and phosphorus from the soil [11,14].…”

Section: Introductionmentioning

confidence: 99%

“…These compounds have diverse functions in plants, such as in protection against phytopathogens and the promotion of growth, which occurs due to the production of such phytohormones as auxin and gibberellins and the absorption of such nutrients as nitrogen and phosphorus from the soil [11,14]. However, there are only a few reports on the fungal endophytes present in ginseng [12,15,16]. Therefore, this study was conducted to explore the diversity of the endophytic fungi in plants of different ages and different tissues of P. ginseng cultivated in Korea.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Fungal Endophytes in Various Tissues of Panax ginseng Meyer Cultivated in Korea

Park¹,

Lee²,

Ahn³

et al. 2012

Journal of Ginseng Research

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Endophytic fungi were isolated from various tissues (root, stem, petiole, leaf, and fl ower stalk) of 3- and 4-year-old ginseng plants (Panax ginseng Meyer) cultivated in Korea. The isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer (ITS), 1-5.8-ITS 2. A morphological characterization was also conducted using microscopic observations. According to the identification, 127 fungal isolates were assigned to 27 taxa. The genera of Phoma, Alternaria and Colletotrichum were the most frequent isolates, followed by Fusarium, Entrophospora and Xylaria. Although 19 of the 27 taxa were identified at the species level, the remainder were classified at the genus level (6 isolates), phylum level (Ascomycota, 1 isolate), and unknown fungal species (1 isolate). Endophytic fungi of 13 and 19 species were isolated from 3- and 4-year-old ginseng plants, respectively, and Phoma radicina and Fusarium solani were the most frequently isolated species colonizing the tissues of the 3- and 4-year-old ginseng plants, respectively. The colonization frequency (CF%) was dependant on the age and tissue examined: the CFs of the roots and stems in the 3-year-old ginseng were higher than the CF of tissues in the 4-year-old plants. In contrast, higher CFs were observed in the leaves and petioles of 4-year-old plants, and endophytic fungi in the flower stalks were only detected in the 4-year-old plants. In conclusion, we detected diverse endophytic fungi in ginseng plants, which were distributed differently depending on the age and tissue examined.

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Chemical constituents from the endophytic fungus Trichoderma ovalisporum isolated from Panax notoginseng

Cited by 27 publications

References 19 publications

Fungal Endophytes from Three Cultivars of Panax ginseng Meyer Cultivated in Korea

Fungal Endophytes from Three Cultivars of Panax ginseng Meyer Cultivated in Korea

Endophytic fungi: a reservoir of antibacterials

Diversity of Fungal Endophytes in Various Tissues of Panax ginseng Meyer Cultivated in Korea

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