Chemical constituents and antibacterial activity of essential oils in <i>Amomum longiligulare</i> from Vietnam

Abstract

This paper reports the chemical constituents and the antibacterial activity of essential oils from the leaves, rhizomes, and fruits of Amomum longiligulare T.L. Wu (Zingiberaceae) obtained by microwave-assisted hydrodistillation. The essential oils were analyzed by gas chromatography–mass spectrometry techniques. The minimum inhibitory concentration (MIC) values were measured by the broth microdilution assay. The oil yields of leaves, rhizomes and fruits from A. longiligulare were 0.23%, 0.27% and 1.93% (v/w), respectively, calculated on a dry weight basis. The leaf essential oil comprised mainly α-humulene (28.4%), α-pinene (24.9%), β-caryophyllene (17.3%), humulene epoxide II (7.3%), and β-pinene (4.7%). The major compounds of the rhizome essential oil were β-caryophyllene (28.7%), bicyclogermacrene (17.1%), humulene epoxide II (10.5%), camphene (7.9%), and α-pinene (5.7%). Camphor (40.7%) and bornyl acetate (34.2%) were the main constituents of the fruit oil. The essential oils demonstrated antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa with the MIC values ranging from 200 to 400 μg/mL. In summary, the A. longiligulare essential oils are a source of promising antibacterial agents. This is the first report on the chemical composition and antibacterial activity of A. longiligulare essential oil obtained by microwave-assisted hydrodistillation.

In Vitro Effect of Purple Amomum (Amomum longiligulare T.L. Wu) Extracts on Seed Germination and Seedling Growth of Different Crop Species

“…Wu) is an important medicinal plant in Vietnam [21,22]. In several phytochemical studies of purple amomum species, the chemical compositions of the volatile oil and non-volatile fractions were determined [21,[23][24][25][26][27][28][29]. Accordingly, the volatile oil of the purple amomum plant was determined to contain abundant amounts of oxidizing monoterpenes (e.g., αpinene, β-pinene, myrcene, camphene, D-limonene, and camphor, etc.)…”

“…Accordingly, the volatile oil of the purple amomum plant was determined to contain abundant amounts of oxidizing monoterpenes (e.g., αpinene, β-pinene, myrcene, camphene, D-limonene, and camphor, etc.) and sesquiterpenes (e.g., caryophyllene and humulene) [21,[26][27][28][29]. Other groups of compounds isolated and identified from purple amomum extracts, in addition to essential oils, include phenolic acids, flavonoids, diarylheptanoids, saponins-steroids, tannins, etc.…”

Antifungal activity of purple amomum (Amomum longiligulare T.L. Wu) plant extracts against Fusarium oxysporum Schlecht. and Helminthosporium sativum P. K. & B.

“…Several compounds isolated from the pseudo-stem and fruit possessed an immune enhancement ability [19][20][21][22]. Essential oils extracted from the leaf, rhizome, and fruit of purple amomum were shown to exhibit antibacterial properties against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa with the minimum inhibitory concentration (MIC) values ranging from 200 to 400 μg/mL [23]. There has been no evaluation of the antifungal activity of purple amomum extracts.…”