Chemical Compositions by Using LC-MS/MS and GC-MS and Biological Activities of Sedum sediforme (Jacq.) Pau

Abstract

In this research, the chemical composition and biological activities of various extracts obtained from whole parts of Sedum sediforme (Jacq.) Pau were compared. The amounts of total phenolic and flavonoid components in crude extracts were determined by expression as pyrocatechol and quercetin equivalents, respectively. All of the extracts (petroleum ether, acetone, methanol, and water) obtained from S. sediforme showed strong antioxidant activity in four tested methods. Particularly, the IC50 values of the methanol extract, which was the richest in terms of total phenolic and flavonoid contents, were found to be lower than those of α-tocopherol and BHT in β-carotene bleaching (9.78 ± 0.06 μg/mL), DPPH free radical scavenging (9.07 ± 0.07 μg/mL), and ABTS cation radical scavenging (5.87 ± 0.03 μg/mL) methods. Furthermore, the methanol extract of S. sediforme showed higher inhibition activity than galanthamine against acetyl- and butyryl-cholinesterase enzymes. Also, acetone and methanol extracts exhibited moderate antimicrobial activity against Candida albicans. The main constituents of fatty acid and essential oil were identified as palmitic acid (C16:0) (28.8%) and α-selinene (20.4%), respectively, by GC-MS. In the methanol extract of S. sediforme, quercetin, rutin, naringenin, and protocatechuic, p-coumaric, caffeic, and chlorogenic acids were detected and quantified by LC-MS/MS. Results of the current study showed that the methanol extract of S. sediforme may also be used as a food supplement.

Phytochemical profile and some biological activities of three Centaurea species from Turkey

“…Several studies are present in literature reporting the use of liquid chromatography electrospray ionization tandem mass spectrometry to perform quantitative analyses [18,19]. In this study, an accurate method on a mass spectrometer equipped with a The antibacterial effect of C. balsamita seen in this study has been reported previously in eight Centaurea species [4,9].…”

“…ESI source was chosen instead of APCI (Atmospheric Pressure Chemical Ionization) and APPI (Atmospheric Pressure Photoionization) sources as the phenolic compounds were small and relatively polar molecules. Tandem mass spectrometry was used for the current study since this system is commonly used for its fragmented ion stability [18] (Table 1). Regression coefficient of each calibration graph was found to be higher than 0.99.…”

Chemical Constituents, Cytotoxic, Antioxidant and Cholinesterases Inhibitory Activities of Silene Compacta (Fischer) Extracts

“…For the studied compounds, LOD ranged from 0.05 to 25.8 µg/L and LOQ ranged from 0.17 to 85.9 µ/L (Table 1). Moreover, the recoveries of the phenolic compounds ranged from 96.9 % to 106.2 % [12,16].…”

“…Several studies are present in literature reporting the use of liquid chromatography electrospray ionization tandem mass spectrometry to perform quantitative analyses of phenolic compounds [12,14,24]. Thus, for quantitative purpose, an accurate method on a mass spectrometer equipped with a triple quadrupole analyzer was developed for the analyses of twenty-four phenolic compounds (flavonoids, flavonoid glycosides, phenolic acids, phenolic aldehyde, coumarin) and three non-phenolic organic acids in the methanol extract of S. compacta methanol extract (SCM).…”

“…However, only this group of drugs is used in the mild treatment to moderate Alzheimer's disease and side effects required make identify new anti-Alzheimer's drugs. With the prolonging human life, the increase of chronic diseases (cancer, cardiovascular disease, Alzheimer's, and so on) has attracted the interest to the antioxidants nowadays [11,12]. On the other hand, synthetic antioxidants are used to extend the shelf life of foods and prevent their degradation.…”