Chemical Compositions, Antioxidant and Antimicrobial Activities of Essential Oils of Piper caninum Blume

Abstract

Chemical composition, antioxidant and antimicrobial activities of the fresh leaves and stems oils of Piper caninum were investigated. A total of forty eight constituents were identified in the leaves (77.9%) and stems (87.0%) oil which were characterized by high proportions of phenylpropanoid, safrole with 17.1% for leaves and 25.5% for stems oil. Antioxidant activities were evaluated by using β-carotene/linoleic acid bleaching, DPPH radical scavenging and total phenolic content. Stems oil showed the highest inhibitory activity towards lipid peroxidation (114.9 ± 0.9%), compared to BHT (95.5 ± 0.5%), while leaves oil showed significant total phenolic content (27.4 ± 0.5 mg GA/g) equivalent to gallic acid. However, the essential oils showed weak activity towards DPPH free-radical scavenging. Evaluation of antimicrobial activity revealed that both oils exhibited strong activity against all bacteria strains with MIC values in the range 62.5 to 250 μg/mL, but weak activity against fungal strains. These findings suggest that the essential oils can be used as antioxidant and antimicrobial agents for therapeutic, nutraceutical industries and food manufactures.

Components, Antioxidant and Antibacterial Activity of Tomato Seed Oil

“…The inhibition value (IC 50 ) observed for the oil was significantly higher than that of BHT due to a low amount of phenolic components in the oil (Salleh et al, 2011). Earlier study has shown that kenaf seed oil might actually possess better antiradical activity if compared with some commercial edible oils (canola oil, corn oil and so on) and it can potentially serve as high antioxidative edible oil.…”

Medicinal Plants of the Indigenous Tribes in Peninsular Malaysia: Current and Future Perspectives

“…Mohd Zin et al [10] had carried out antioxidative activity of extracts from Morinda citrifolia L. and had concluded that active compounds in root of the plant might be both polar and nonpolar in nature, whereas compounds that contribute to antioxidative activity of both its leaf and fruit are probably nonpolar in nature. Hakimi Wan Salleh et al [11] studied the chemical compositions and antioxidant and antimicrobial activities of essential oils of Piper caninum Blume. Safrole, β-caryophyllene, β-pinene, and germacrene D were the main components from the leave and stem oil of the plant.…”

Chemical composition and biological activities of essential oil ofBeilschmiedia pulverulenta

“…Antimicrobial tests were determined by the disk diffusion method described by Gulluce et al (2004) with slight modifications as described by Salleh et al (2011). Briefly, 400 mL suspension containing 10 8 CFU/mL bacteria and 10 6 CFU/mL fungal/yeast were spread on NA and SDA mediums, respectively.…”

“…The MIC and MBC were determined by the broth microdilution method using 96-well microplates described by Gulluce et al (2004) with modifications stated by Salleh et al (2011). The inocula of the microbial strains were prepared from 24 h broth cultures and suspensions were adjusted to 0.5 McFarland standard turbidity.…”