Chemical compositions and bioactivities of crude polysaccharides from tea leaves beyond their useful date

Xiao, Jianbo; Huo, Jianglei; Jiang, Hao; Yang, Fan

doi:10.1016/j.ijbiomac.2011.09.013

Cited by 77 publications

(41 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The α-glucosidase inhibitors are mostly evaluated by determination of α-glucosidase inhibitory activity using pNPG as the reaction substrate (Xiao et al, 2011). However, so far there is little information published about the α-amylase and α-glucosidase inhibitory activity of polysaccharides from L. japonica.…”

Section: Inhibitory Activity Of α-Amylase Andmentioning

confidence: 99%

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Wan

Yang

Cai

et al. 2015

J. Ocean Univ. China

View full text Add to dashboard Cite

In the present study, ultrasonic extraction technique (UET) is used to improve the yield of polysaccharides from Laminaria japonica (LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design (BBD) combined with response surface methodology (RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80℃ and ratio of material to solvent at 1:50 (g mL −1 ). Under these optimal ultrasonic extraction conditions, an actual experimental yield (5.75% ± 0.3%) is close to the predicted result (5.67%) with no significant difference (P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides (LJP) and two major ethanol precipitated fractions (LJP1 and LJP2) are in a concentration-dependent manner. LJP2 (30%60% ethanol precipitated polysaccharides) possesses the strongest α-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals (66.09% ± 2.19%, 3.0 mg mL −1 ). Also, the inhibitory activity against α-glucosidase (59.08% ± 3.79%, 5.0 mg mL −1 ) is close to that of acarbose (63.99% ± 3.27%, 5.0 mg mL −1 ). LJP1 (30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals (99.80% ± 0.00%, 3.0 mg mL −1 ) and moderate α-glucosidase inhibitory activity (47.76% ± 1.92%, 5.0 mg mL −1 ). LJP shows the most remarkable DPPH scavenging activity (66.20% ± 0.11%, 5.0 mg mL −1 ) but weakest α-glucosidase inhibitory activity (37.77% ± 1.30%, 5.0 mg mL −1 ). However, all these LJPs exert weak inhibitory effects against α-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provides scientific evidence and advances in the preparation technology and a hypoglycemic activities evaluation method for seaweed polysaccharides, especially glycosidase inhibition in combination with an antioxidative activity evaluation method.

show abstract

Section: Inhibitory Activity Of α-Amylase Andmentioning

confidence: 99%

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Wan

Yang

Cai

et al. 2015

J. Ocean Univ. China

View full text Add to dashboard Cite

show abstract

“…Polysaccharides are very common natural polymers in plants, animals and microorganisms (Xiao et al 2011, Cui et al 2013. Recently, polysaccharides from plants have attracted more and more attention due to their extensive biological activities, such as hypoglycemic activity (Wang et al 2001), free-radical-scavenging activity (Cui et al 2013), anticancer activity (Xie et al 2013), and improvement of immunomodulation activity (Huang & Ning 2010).…”

Section: Introductionmentioning

confidence: 99%

Variation in growth, photosynthesis and water-soluble polysaccharide of Cyclocarya paliurus under different light regimes

Yang¹,

Liu²,

Fang³

et al. 2017

iForest

View full text Add to dashboard Cite

(1-2) As a highly valued and multiple function tree species, Cyclocarya paliurus is planted and managed for timber production and medical use. Responses of growth, photosynthesis and phytochemical accumulation to light environment are useful informations to determine suitable habitat conditions for the cultivation of C. paliurus. A split-plot design with five light quality and three light intensity levels was adopted to compare the variations in plant growth, photosynthesis and water-soluble polysaccharide yield in C. paliurus leaves. Both light intensity and quality treatments significantly affected total biomass, photosynthetic rate and water-soluble polysaccharide yield in C. paliurus leaves. Treatments under red light and blue light with 1000 μmol m -2 s -1 achieved the highest values of biomass growth, photosynthetic rate, specific dry leaf mass per area and accumulation of water-soluble polysaccharide. These results indicate that red light and blue light with higher light intensity level were effective for increasing plant growth, photosynthesis and production of water-soluble polysaccharide in C. paliurus leaves. Manipulating light conditions might be an effective means to improve biomass and achieve higher water-soluble polysaccharide yield in C. paliurus plantations.

show abstract

“…Several studies related to polysaccharides have drawn the attention of chemists and immunobiologists in recent years on account of their immunomodulation and anti-virus properties. Meanwhile, their antibacterial activities are worthy of further research [12,13].…”

Section: Introductionmentioning

confidence: 99%

Extraction, purification and antibacterial activity of polysaccharides from Purslane

Gu¹,

Shu²

2017

Proceedings of the 2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017)

View full text Add to dashboard Cite

Abstract. In this study, the polysaccharides from Purslane were extracted by ultrasonic method and its content was measured by the phenol-sulfuric acid method using glucose as the standard. The crude polysaccharides were collected and purified by DEAE-Cellulose anion-exchange and Sephadex gel-permeation chromatography. Three fractions were obtained and named POL1, POL2 and POL3, respectively. The molecular weights of these three fractions were measured by Sephadex using dextran standard curve. Escherichia coli, Acetic acid bacteria, Saccharomycetes and Aspergillus Niger were used to study the antibacterial activity of the polysaccharides. The results showed that molecular weights of POL1, POL2 and POL3 were 18Kd, 55kd and 108kd, respectively. The polysaccharides had inhibitory effect on microorganisms, and the inhibited order of microorganisms was: Saccharomycetes>Acetic acid bacteria>Aspergillus Niger>Escherichia coli. In addition, the order of antibacterial activities of these three fractions on microorganisms was: POL3>POL2b>POL1b. It could be concluded that three fractions were obtained by purification. These three fractions had inhibitory effect on Saccharomycetes, Acetic acid bacteria, Aspergillus Niger and Escherichia coli. The antibacterial activity of polysaccharides from Purslane against Saccharomycetes was the strongest, while the weakest against Escherichia coli. In addition, POL3 was found to be the most effective against the tested microorganisms among the three fractions.

show abstract

Chemical compositions and bioactivities of crude polysaccharides from tea leaves beyond their useful date

Cited by 77 publications

References 28 publications

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Variation in growth, photosynthesis and water-soluble polysaccharide of Cyclocarya paliurus under different light regimes

Extraction, purification and antibacterial activity of polysaccharides from Purslane

Contact Info

Product

Resources

About