Chemical composition of the essential oil and n-hexane extract of Stachys tmolea subsp. Tmolea Boiss., an endemic species of Turkey, and their mosquitocidal activity against dengue vector Aesdes aegypti

Demiray, Hatice; Tabanca, Nurhayat; Estep, Alden S.; Becnel, James J.; Demirci, Betül

doi:10.1016/j.jsps.2019.05.009

Cited by 12 publications

(8 citation statements)

References 29 publications

(35 reference statements)

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“…Also, diterpenes are an important group of compounds with anti-inflammatory activities [19,20]. Phytol is an acyclic diterpene alcohol, commonly found in the composition of various oils [21][22][23], which was found among the bioactive metabolites of P. russeliana n-hexane sub-extract, associated with anti-inflammatory activity [24]. Thus, in the present study, the remarkable anti-inflammatory activity of the bioactive fraction may be influenced from the phytol content.…”

Section: Bioactivity-guided Fractionation and Chemical Characterizationmentioning

confidence: 64%

“…The identifications of the oil components were performed by comparison to their relative retention times (RRT) with those of authentic samples or compared to their relative retention index (RRI) to series of n-alkanes. The computer matching of the separated compounds against commercial (Wiley GC/MS Library, MassFinder Software 4.0) and the in-house "Başer Library of Essential Oil Constituents" built up by genuine compounds and components from literature previous reported [3,4,21]. Folin-Ciocalteu method was used for the determination of the total phenolics content of P. russeliana MeOH extract.…”

Section: Gc-fid and Gc/ms Analysesmentioning

confidence: 99%

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In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

et al. 2020

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The air-dried aerial parts of Phlomis russeliana (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by n-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of P. russeliana extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the P. russeliana methanol extract showed LOX inhibitory activity at IC50 = 23.2 µg/mL, whereas the DPPH• assay showed IC50 = 0.89 mg/mL, and the ABTS• assay showed IC50 = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC–FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the P. russeliana extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of Phlomis species with further potential.

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Section: Bioactivity-guided Fractionation and Chemical Characterizationmentioning

confidence: 64%

Section: Gc-fid and Gc/ms Analysesmentioning

confidence: 99%

In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

et al. 2020

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“…However, similar activity was also observed against the non-carcinoma cell line HaCaT, indicating that it may not possess cancer-specific antiproliferative activity, although further studies are required. Although main components of the EO have been reported as main constituents in other plant extracts with similar biological activities [ 43 , 44 , 45 ], there are no available studies on the biological activities of the individual compounds, which is a valid area of further investigation. Overall, the results of our study indicated that EO form S. raeseri subsp.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritis raeseri subps. raeseri Essential Oil

Mitropoulou

Sidira²,

Skitsa

et al. 2020

Foods

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The aim of the present study was to investigate the antimicrobial potential of Sideritis raeseri subps. raeseri essential oil (EO) against common food spoilage and pathogenic microorganisms and evaluate its antioxidant and antiproliferative activity. The EO was isolated by steam distillation and analyzed by GC/MS. The main constituents identified were geranyl-p-cymene (25.08%), geranyl-γ-terpinene (15.17%), and geranyl-linalool (14.04%). Initially, its activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis, Salmonella Typhimurium, Pseudomonas fragi, Saccharomyces cerevisiae, and Aspergillus niger was screened by the disk diffusion method. Subsequently, minimum inhibitory concentration (MIC), non-inhibitory concentration (NIC), and minimum lethal concentration (MLC) values were determined. Growth inhibition of all microorganisms tested was documented, although it was significantly lower compared to gentamycin, ciproxin, and voriconazole, which were used as positive controls. In a next step, its direct antioxidant properties were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, and the IC50 values were determined. The potential cytoprotective activity of the oil against H2O2–induced oxidative stress and DNA damage was studied in human immortalized keratinocyte (HaCaT) cells using the comet assay. Finally, the antiproliferative activity of the oil was evaluated against a panel of cancer cell lines including A375, Caco2, PC3, and DU145 and the non-cancerous HaCaT cell line using the sulforhodamine B (SRB) assay, and the EC50 values were determined. The oil demonstrated weak radical scavenging activity, noteworthy cytoprotective activity against H2O2–induced oxidative stress and DNA damage in HaCaT cells, and antiproliferative activity against all cell lines tested, being more sensitive against the in vitro model of skin melanoma.

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“…Additionally, hexahydrofarnesyl acetone represented the predominated compound in all overall carotenoid-derived compounds. Hexahydrofarnesyl acetone is a common apocarotenoid-derived compound in EOs derived from the plant kingdom such as Hildegardia barteri [34], Stachys tmolea [35], and Bassia muricata [36].…”

Section: Yields and Chemical Constituents Of P Undulata Eosmentioning

confidence: 99%

Comparative Chemical Profiles and Phytotoxic Activity of Essential Oils of Two Ecospecies of Pulicaria undulata (L.) C.A.Mey

Abd‐ElGawad

Al‐Rowaily

Assaeed

et al. 2021

Plants

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The Asteraceae (Compositae) family is one of the largest angiosperm families that has a large number of aromatic species. Pulicaria undulata is a well-known medicinal plant that is used in the treatment of various diseases due to its essential oil (EO). The EO of both Saudi and Egyptian ecospecies were extracted via hydrodistillation, and the chemical compounds were identified by GC–MS analysis. The composition of the EOs of Saudi and Egyptian ecospecies, as well as other reported ecospecies, were chemometrically analyzed. Additionally, the phytotoxic activity of the extracted EOs was tested against the weeds Dactyloctenium aegyptium and Bidens pilosa. In total, 80 compounds were identified from both ecospecies, of which 61 were Saudi ecospecies, with a preponderance of β-pinene, isoshyobunone, 6-epi-shyobunol, α-pinene, and α-terpinolene. However, the Egyptian ecospecies attained a lower number (34 compounds), with spathulenol, hexahydrofarnesyl acetone, α-bisabolol, and τ--cadinol as the main compounds. The chemometric analysis revealed that the studied ecospecies and other reported species were different in their composition. This variation could be attributed to the difference in the environmental and climatic conditions. The EO of the Egyptian ecospecies showed more phytotoxic activity against D. aegyptium and B. pilosa than the Saudi ecospecies. This variation might be ascribed to the difference in their major constituents. Therefore, further study is recommended for the characterization of authentic materials of these compounds as allelochemicals against various weeds, either singular or in combination.

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Chemical composition of the essential oil and n-hexane extract of Stachys tmolea subsp. Tmolea Boiss., an endemic species of Turkey, and their mosquitocidal activity against dengue vector Aesdes aegypti

Cited by 12 publications

References 29 publications

In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritis raeseri subps. raeseri Essential Oil

Comparative Chemical Profiles and Phytotoxic Activity of Essential Oils of Two Ecospecies of Pulicaria undulata (L.) C.A.Mey

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