Chemical composition of Nigella sativa L. seed extracts obtained by supercritical carbon dioxide

Venkatachallam, Suresh Kumar Tiruppur; Pattekhan, H. H.; Divakar, Soundar; Kadimi, Udaya Sankar

doi:10.1007/s13197-010-0109-y

Cited by 124 publications

(78 citation statements)

References 19 publications

(22 reference statements)

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“…46 The active ingredients of N. sativa have beneficial effects against many diseases, including inflammation, hypertension, diabetes, headache, fever, and gastrointestinal disturbances. 4 Modern pharmacological and toxicological studies have demonstrated that TQ, the most abundant and bioactive constituent of oil extract of N. sativa, exerts antioxidant effects, 27 antiinflammatory effects, 16 and antitumor activity.…”

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confidence: 99%

Neuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosis

Gökçe

Kahveci

Gökçe³

et al. 2016

SPI

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OBJECTIVE Ischemia-reperfusion (I/R) injury of the spinal cord following thoracoabdominal aortic surgery remains the most devastating complication, with a life-changing impact on the patient. Thymoquinone (TQ), the main constituent of the volatile oil from Nigella sativa seeds, is reported to possess strong antioxidant, antiinflammatory, and antiapoptotic properties. This study investigated the effects of TQ administration following I/R injury to the spinal cord. METHODS Thirty-two rats were randomly allocated into 4 groups. Group 1 underwent only laparotomy. For Group 2, aortic clip occlusion was introduced to produce I/R injury. Group 3 was given 30 mg/kg of methylprednisolone intraperitoneally immediately after the I/R injury. Group 4 was given 10 mg/kg of TQ intraperitoneally for 7 days before induction of spinal cord I/R injury, and administration was continued until the animal was euthanized. Locomotor function (Basso, Beattie, and Bresnahan scale and inclined plane test) was assessed at 24 hours postischemia. Spinal cord tissue samples were harvested to analyze tissue concentrations of malondialdehyde, nitric oxide, tumor necrosis factor–α, interleukin-1, superoxide dismutase, glutathione-peroxidase, catalase, and caspase-3. In addition, histological and ultrastructural evaluations were performed. RESULTS Thymoquinone treatment improved neurological outcome, which was supported by decreased levels of oxidative products (malondialdehyde and nitric oxide) and proinflammatory cytokines (tumor necrosis factor–α and interleukin-1), increased activities of antioxidant enzymes (superoxide dismutase, glutathione-peroxidase, and catalase), as well as reduction of motor neuron apoptosis. Light microscopy and electron microscopy results also showed preservation of tissue structure in the treatment group. CONCLUSIONS As shown by functional, biochemical, histological, and ultrastructural analysis, TQ exhibits an important protective effect against I/R injury of the spinal cord.

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confidence: 99%

Neuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosis

Gökçe

Kahveci

Gökçe³

et al. 2016

SPI

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“…Nigella sativa (black cumin) seeds, a dicotyledon of Ranunculaceae family, have been used for thousands years as spice and food preservative [1][2][3]. Black cumin is an annual herbaceous plant widely grown in Mediterranean countries, Middle East, Eastern Europe and Western Asia.…”

Section: Introductionmentioning

confidence: 99%

“…Black cumin is an annual herbaceous plant widely grown in Mediterranean countries, Middle East, Eastern Europe and Western Asia. In Middle East, Northern Africa and India, it has been used traditionally for centuries to treat asthma, cough, bronchitis, headache, rheumatism, fever, influenza and eczema [3,4]. It has also been used as anti-histamine, anti-diabetes and anti-inflammatory.…”

Section: Introductionmentioning

confidence: 99%

“…The oil and seed constituents, in particular thymoquinone (TQ), have shown medicinal properties in treatment and prevention of various diseases [5][6][7][10][11][12]. They have various potent activities such as anti-inflammatory [3]; anti-oxidant [1,2,4]; anti-cancer [6,7,10], etc. In addition, they also expressed anti-microbial properties toward different microbes [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Supercritical fluid carbon dioxide extraction of Nigella sativa (black cumin) seeds using taguchi method and full factorial design

Salea¹,

Widjojokusumo²,

Hartanti³

et al. 2013

Bio Chem Comp

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Optimum condition for Nigella sativa seeds oil and its bioactive compound, thymoquinone (TQ) using supercritical fluid carbon dioxide extraction (SCFE-CO 2 ), were investigated. The optimization process was performed with Taguchi method and full factorial design (FFD) under the following condition: pressure (150, 200 and 250 bar), temperatures (40, 50 and 60 o C) and carbon dioxide (CO 2 ) flowrate (10, 15 and 20 g/min), in which solvent to feed (SF) ratio was set constant at 24. The highest yield of Nigella sativa seeds oil from SCFE-CO 2 process with FFD method was 12% at 250 bar, 60 o C and 20 g/min. Whereas Taguchi method was performed at 250 bar, 50 o C, 10 g/min with oil yield 11.9%. The highest thymoquinone content in Nigella sativa seeds oil from both experimental design was achieved through SCFE-CO 2 extraction condition at 150 bar, 60 o C and 20 g/min with thymoquinone content 20.8 mg/ml. In addition, conventional methods such as high pressure soxhlet with liquid CO 2 , n-hexane soxhlet and percolation with ethanol were performed with oil yield 5.8%, 19.1% and 12.4%; and thymoquinone content 8.8 mg/g oil, 6.3 mg/g oil and 5.0 mg/g oil, respectively. The analysis of variance (ANOVA) with 95% confidence interval, indicates effect of pressure on Nigella sativa seeds oil yield and thymoquinone content from SCFE-CO 2 process. The oil was then evaluated for its activity. The antibacterial activity of the oil from Taguchi Method, shows that all samples were unable to inhibit Escherichia coli O157 and Salmonella typhimurium. For other pathogenic bacterias, all samples show similar inhibition at concentration 10% oil for MRSA, 3% oil for Stapylococcus aureus and 3% oil for Bacillus subtilis.

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“…experimental studies have indicated its anti-histaminic, hypocholesterolemic, antioxidant and antiinflammatory potential, anti-convelsent, anti cancer and anti-microbial activities. Interestingly the polar fraction of these seeds, extracted by methanol, also have antioxidant and anti-inflammatory potential 39 . Recently its radioprotection role has also been reported 40 .…”

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Radiation Induced Gastrointestinal Damage and Protection: Nigella Sativa Seed Extract and Thymoquinone

et al. 2017

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Ionising radiation therapy is a common treatment for different types of cancers. The side effects associated with radiation includes destruction of normal cells, especially the dividing cells. The cells in the gastrointestinal (GI) tract and bone marrow are the primary targets. The GI damage is reflected by early histological changes, functional alterations and symptoms of nausea, vomiting and diarrhea. This has been designated as the radiation syndrome. Many synthetic drugs have been used to treat GI disorders but a definite cure has not been discovered so far and these available medications also cause several side effects. The herbal extracts are being tested for long time as preventive food supplement/drug in this disease. The radio protective effects of Nigella sativa (black cumin, (Ranunculacea) is already reported but its mechanism of action is not well established. Here in this review this aspect has been explored with special reference to various in vitro and in vivo models.Keyword: Nigella sativa; Thymoquinone; Radioprotector; Herbal; Radiation Defence Life Science Journal, Vol. 2, No. 3, July 2017, pp. 264-269, DOI : 10.14429 REVIEW PAPER265 PANDAY, et al.: DEf. LIfE SCI. J., VOL. 2, NO. 3, JuLY 2017, DOI : 10.14429/dlsj.2.11665 cancers of the skin, cervix, prostate, lungs, thyroid and brain), and may be the only treatment needed to cure the cancer 7 . Nevertheless, radiation therapy remains dose limited by the tolerance of surrounding normal tissues.The gastrointestinal tract (GIT) not only is a major doselimiting organ during abdominal, pelvic, and retroperitoneal radiation therapy 8 but is also one of organs for the outcome of accidental whole-body radiation exposure. An exposure to the dose of 5 Gy -8 Gy, may result to death within few weeks. However low dose in the range of 2 Gy -5 Gy, may result to delayed symptoms especially to the hematopoietic system, resulting immunosuppression and rapid manifestation of infections. The GI damage is another cause of death depending on the dose and severity of damage, including intestinal crypt cell death. Therefore, interventions that reduce gastrointestinal radiation injury are urgently needed. Radiation enteritis is a challenging clinical problem in patients receiving ionising radiation 9 . Clinically, irradiation can result in several deleterious gastro-intestinal symptoms such as bleeding, anorexia, nausea, vomiting and diarrhea i.e. gastrointestinal radiation syndrome 10,11 . The high sensitivity of the GIT to ionising radiation (and other cytotoxic insults) is a dose limiting issue in some radio-and chemotherapy practices. The duodenum is the most radiosensitive region of the digestive tract, followed by jejunum, ileum, esophagus, stomach, colon, and rectum, in order of radiosensetivity 12,13 .The mucosal cells are characterised by rapid proliferation rate and relatively high degree of radiosensetivity. In both animal models and patients, small intestinal and whole gut transit was markedly accelerated within hours after irradiation 14,15 . ...

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Chemical composition of Nigella sativa L. seed extracts obtained by supercritical carbon dioxide

Cited by 124 publications

References 19 publications

Neuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosis

Neuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosis

Supercritical fluid carbon dioxide extraction of Nigella sativa (black cumin) seeds using taguchi method and full factorial design

Radiation Induced Gastrointestinal Damage and Protection: Nigella Sativa Seed Extract and Thymoquinone

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