Chemical composition of human bone

Quelch, Kaylene J.; Melick, R. A.; Bingham, Patricia J.; Mercuri, Susan M.

doi:10.1016/0003-9969(83)90100-0

Cited by 52 publications

(33 citation statements)

References 31 publications

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“…Alpha2HS-glycoprotein has a strong affinity for the mineral phase of bone [2, I1, 13]. However, our results suggest that the rate of bone turnover is more important than the mineral content in determining the concentration of %HS-glycoprotein in bone because the highly calcified adult bone [8] and osteopetrotic bone contained the lowest levels of this protein. Albumin has a lower affinity for the mineral phase [2,11] and its concentration did not always follow changes in the concentration of o~2HS-glycoprotein in the diseases studied.…”

Section: Discussioncontrasting

confidence: 71%

“…As both young and abnormal bone contain more rather than less water [8], [Quelch and Melick, unpublished] the results, if expressed relative to the dry weight, would increase the differences found.…”

Section: Resultsmentioning

confidence: 73%

“…Proteinase inhibitors were not used but we showed, using two labeled substrates, that the collagenase (type VII obtained from Sigma Chemical Co. St l.ouis. Mo.. U.S.A. was free of proteinase activity [8]. The digest was dialyzed and the retained soluble noncollagenous pro- Values shown are the mean _+ SE, with the number of patients in parentheses teins were lyophilized and analyzed for their contents of e~zHS-glycoprotein, albnmin, sialic acid, and total protein.…”

Section: Methodsmentioning

confidence: 99%

“…AlphazHSglycoprotein and albumin were measured by radial immunodiffusion using Partigen plates (Behringwerke, Marburg-Lahn, West Germany) containing antiserum to human c~2HS-glycoprotein or to albumin [7]. Sialic acid and water content were measured by previously described methods [8]. The total protein content of the noncollagenous matrix was determined by the method of Lowry et al [9].…”

Section: Methodsmentioning

confidence: 99%

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Noncollagenous proteins in normal and pathological human bone

1984

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The concentrations of alpha 2HS-glycoprotein, albumin, and sialic acid were measured in the bone of 28 normal individuals and 6 patients with osteogenesis imperfecta, 3 patients with Paget's disease, and 4 patients with either renal osteodystrophy, osteoporosis, osteomalacia, or osteopetrosis. The concentration of alpha 2HS-glycoprotein in neonatal bone was 3 X higher than in bone from children and 7 X higher than in adult bone. The concentrations of albumin and sialic acid in neonatal bone were 1.5 X higher that in bone from children and twice the levels in adult bone. The concentrations of alpha 2HS-glycoprotein, albumin and sialic acid were above the normal mean values in the patients with osteogenesis imperfecta, and 4 patients had significantly raised levels of one or more of these proteins. The concentrations of these noncollagenous proteins were also significantly raised in Paget's disease, and the alpha 2HS-glycoprotein was significantly raised in renal osteodystrophy. The lowest level of alpha 2HS-glycoprotein was in osteopetrotic bone and the lowest levels of albumin and sialic acid were in osteoporotic bone. The results of this study suggest that the concentrations of alpha 2HS-glycoprotein, albumin, and sialic acid in bone are related to the rate of bone turnover.

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Section: Discussioncontrasting

confidence: 71%

Section: Resultsmentioning

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Noncollagenous proteins in normal and pathological human bone

1984

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“…Dentin has a similar extracellular matrix consisting approximately of 70% mineral components, dominated mainly by calcium and phosphate (Quelch et al, 1983). In addition, the extracellular matrix proteins of bone and dentin are comparable, consisting primarily of type I collagen, acidic proteins and proteoglycans (Butler et al, 1997).…”

Section: Discussionmentioning

confidence: 99%