Chemical composition of Histoplasma capsulatum

Cino, P. M.; Tewari, Ram P.

doi:10.1007/bf02051666

Cited by 9 publications

(2 citation statements)

References 26 publications

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“…The number of yeast cells that would contain 100 ug of ribosomal RNA (or ribosomal protein) was calculated after performing the chemical analysis of early log yeast cells of H capsulatum (Cino and Tewari, 1972). The yeast cells of Histoplasma contained 6.8% total RNA and the dry weight of 1x109 yeast cells was 329 mg. From these data it was estimated that approximately 5x106 to 6x106 To determine the immunogenicity of ribosomes isolated from formalinized cells, mice were immunized s.c. with varying doses of ribosomes with or without FIA.…”

Section: Comparativementioning

confidence: 99%

Immunogenicity of subcellular fractions from yeast cells of Histoplasma capsulatum.

Tewari

LaFemina

1983

Jpn J Med Mycol

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We have previously demonstrated a high degree of protection in mice immunized with partially purified ribosomes and ribosomal protein from H. capsulatum. In order to define more precisely the nature of the protective antigen(s), we have further investigated the immunogenicity of different subcellular fractions from yeast cells of H. capsulatum. The ribosomes and a membrane-rich cytoplasmic fraction from Histoplasma protected 90-100% of the immunized mice against a lethal challenge with yeast cells of H. capsulatum. The ribosomes incorporated 14C-L-phenylalanine in a poly U directed cell-free protein synthesizing system and could be dissociated into 40 and 60S subunits. Significant but somewhat lower levels of protection were obtained with the mitochondrial fractions. In contrast, only 30-50% protection was observed with cell wall preparations and 20-40% protection with microsomal and ribosomal supernates. High levels of protective immunity were observed when mice were immunized with combination of suboptimal doses of ribosomes and cell walls. Immunization with ribosomes from Saccharomyces cerevisiae and Candida albicans in combination with Histoplasma cell walls did not elicit significant protection against H. capsulatum, indicating the specific nature of the immune response. These results demonstrate that ribosomes and membrane-rich cytoplasmic fractions are the major protective cellular constituents of Histoplasma. Furthermore, the combination of purified immunogenic subcellular constituents in the ritht proportion may provide a safe and effective veccine against histoplasmosis.

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Section: Comparativementioning

confidence: 99%

Immunogenicity of subcellular fractions from yeast cells of Histoplasma capsulatum.

Tewari

LaFemina

1983

Jpn J Med Mycol

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“…Both morphologic forms can be cultured in vitro under controlled conditions. There have been several studies which have compared physiological or chemical characteristics of the two phases but none have attempted to characterize biochemical events during yeast to mycelial (Y -+ M) or mycelial to yeast (M + Y) transitions (Cino & Tewari, 1972;Gupta & Howard, 1971 ;Kobayashi & Guiliacci, 1967;Mahvi, 1965). As a first step in the characterization of these morphogenetic processes, we have examined RNA metabolism of the yeast, mycelial and intermediate stages during the M + Y transition.…”

Section: Introductionmentioning

confidence: 99%

RNA Metabolism during Morphogenesis in Histoplasma capsulatum

Cheung

Kobayashi

Schlessinger

et al. 1974

Journal of General Microbiology

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SUMMARYDuring the transition from the mycelial to the yeast form of the dimorphic fungus Histoplasma capsulatum, RNA synthesis decreased to 26.7 % of the normal level for at least 4h. There was a decrease in total RNA on the first day of mycelial +yeast transition. Starting on the second day, RNA synthesis in the mycelial -+ yeast transition phase resumed and this event was paralleled by the emergence of bud-like constrictions on hyphal tips.

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Aspects of physiology of Histoplasma capsulatum (A review)

Boguslawski

Stetler

1979

Mycopathologia

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Chemical composition of Histoplasma capsulatum

Cited by 9 publications

References 26 publications

Immunogenicity of subcellular fractions from yeast cells of Histoplasma capsulatum.

Immunogenicity of subcellular fractions from yeast cells of Histoplasma capsulatum.

RNA Metabolism during Morphogenesis in Histoplasma capsulatum

Aspects of physiology of Histoplasma capsulatum (A review)

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