Chemical Composition of Essential Oils From Vitex Agnus-Castus L. Growing in Morocco and Its in Vitro Antibacterial Activity Against Clinical Bacteria Responsible for Nosocomial Infections

Kamari, Fatima El; Taroq, Amal; Atki, Yassine El; Aouam, Imane; Lyoussi, Badiâa; Abdellaoui, Abdelfattah

doi:10.22159/ajpcr.2018.v11i10.27307

Cited by 10 publications

(16 citation statements)

References 24 publications

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“…These results are in agreement with previous studies 10 . The essential oil extracted from different aromatic plants has an antibacterial power 23,24 . The essential oil of the Lebanese laurel shows antibacterial activity against the tested bacteria 9 .…”

Section: Resultsmentioning

confidence: 99%

“…While Grampositive bacteria included only Staphylococcus aureus (S. aureus). For the bacterial susceptibility screening test, we used the agar-disc diffusion method as mentioned earlier 23,24 . Each microorganism stock was suspended in Mueller-Hinton (MH) broth and then incubated at 37 °C for 18-24 h. The overnight cultures were diluted and adjusted to get a density of 100 CFU/ml (0.5 McFarland turbidity standards).…”

Section: Identification Of Essential Oil Constituents By Gas Chromatomentioning

confidence: 99%

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2019

IJPSR

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Bacterial resistance and oxidative stress are an important etiology of chronic diseases. This is the reason why new alternatives were established to overcome the incidence of some pathologies. Thus, the present study was aimed to evaluate the antibacterial activity of the extracted oil against bacteria that causes nosocomial infections in neonatal intensive care by using disc diffusion method. The antioxidant activity was evaluated using various methods (1,1-diphenyl-2picrylhydrazyl (DPPH) radical scavenging, reducing power assay, total antioxidant activity) and the content of total phenolic and flavonoids were determined. In the essential oil, the twenty-eight compound was identified by GC/MS analysis. The major components were: 1,8-cineol (26.76%), 1-α-pinene (8.63%), linalool (7.78%). The oil strongly reduced the DPPH radical (IC 50 = 4. 4 µg/ml) and reducing of molybdate (0.53 ± 0.0027 mg Eq ascorbic acid/µg EO. The oil showed strong antibacterial activity against nosocomial bacteria tested. This study confirms the essential oil of laurus nobilis. L processes antibacterial and antioxidant properties in-vitro.

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Section: Resultsmentioning

confidence: 99%

Section: Identification Of Essential Oil Constituents By Gas Chromatomentioning

confidence: 99%

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2019

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“…Antibacterial effect was tested by measuring the zones of inhibition. The antibiotic standards used were imipenem, ampicillin, pristinamycin, and ceftriaxone [26][27][28]. The tests were carried out in triplicates.…”

Section: Antibacterial Activity Assessmentmentioning

confidence: 99%

Chemical Composition, Antimicrobial, and Antioxidant Activities of Two Moroccan Thymus Essential Oils

Aouam

Atki

Taroq

et al. 2019

Asian J Pharm Clin Res

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Objective: The aim of the present study is to investigate chemical constituents of Thymus riatarum and Thymus blecherianus essential oils (EOs) as well as to evaluate, for the first time, their antioxidant effect and antibacterial activity against six bacterial strains responsible for nosocomial infections.Methods: The chemical composition of EOs was analyzed using gas chromatography (GC) and GC-mass spectrometry, the antibacterial capacity of the two thymus species was evaluated against six bacteria species: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Acinetobacter baumannii, and Citrobacter sp. using disk diffusion method and microdilution assay. Finally, the antioxidant activity was measured by four different test systems of assay, namely free radical scavenging activities, ferric reducing/antioxidant power assays, total phenolic, and flavonoid contents.Results: A total of 15 compounds representing 99.6% of T. riatarum oil were identified with thymol (28.8%), borneol (20.0%), and α-phellandrene (13.0%) as the main constituents. Eleven components of T. blecherianus were identified representing 98.2% of the total oil composition; the most abundant constituents were as follows: Carvacrol (45.9%), bornyl acetate (20.1%), and borneol (15.7%). Strong antibacterial activity of the two EOs was identified against all bacterial strains tested. Concerning the antioxidant results, T. riatarum EO exhibited higher antioxidant activity than T. blecherianus in the three assays with an IC50 value equal to 5.75±0.06 mg/ml, which was probably due to its high content of polyphenols (28.95±0.13 mg GAE/g DW). Total flavonoid content was found equal for the two EOs.Conclusion: EO of T. riatarum and T. blecherianus from Morocco can be exploited as a natural antibacterial and antioxidant new potential sources.

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“…Vitex agnus-castus is a well-known herbal plant (included in the European Pharmacopoeia) which is rich in numerous bioactive substances. A number of studies have been applied to establishing the chemical composition of the essential oils (EOs) obtained from V. agnus-castus fruits [ 3 , 4 ]. These show variation in the yield of EOs and their composition, related to the habitat conditions [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Antimicrobial Activity of Essential Oil of Fruits from Vitex agnus-castus L., Growing in Two Regions in Bulgaria

Zhelev

Petkova

Kostova³

et al. 2022

Plants

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The chemical composition of Vitex agnus-castus L. (Verbenaceae family) fruits, collected from two regions in Bulgaria (south-central and north-east Bulgaria), was investigated. The content of proteins (5.3–7.4%), carbohydrates (73.9–78.8%), fiber (47.2–49.9%), ash (2.5–3.0%), essential oils (0.5%), and vegetable oil (3.8–5.0%) were identified in the fruits. The composition of the essential oils (EOs) of Vitex fruits from both regions was determined; the main compounds were 1,8-cineole (16.9–18.8%), α-pinene (7.2–16.6%), sabinene (6.7–14.5%), and bicyclogermacrene (7.3–9.0%), but significant differences in the quantitative and qualitative composition of EOs between the regions were found. The EOs of plants from north-east Bulgaria demonstrated antimicrobial activity against the pathogenic species Salmonella abony, Staphylococcus aureus, and Bacillus subtilis, but the Gram-negative bacteria Esсherichia coli and Pseudomonas aeruginosa exhibited resistance to the oil. Linoleic acid predominated in vegetable oil from both regions, followed by oleic acid. β-sitosterol and γ-tocopherol were the main components in the sterol and tocopherol fraction of the lipids. Phosphatidic acids were the main components in the vegetable oil from north-east Bulgaria, while in the vegetable oil from south-central Bulgaria, all phospholipids were found in almost the same quantity. Overall, significant differences were observed in the chemical composition (proteins, carbohydrates, ash and moisture) of the fruits from the two regions of Bulgaria, as well as in the content of the main components of their essential and vegetable oils.

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Chemical Composition of Essential Oils From Vitex Agnus-Castus L. Growing in Morocco and Its in Vitro Antibacterial Activity Against Clinical Bacteria Responsible for Nosocomial Infections

Cited by 10 publications

References 24 publications

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Chemical Composition, Antimicrobial, and Antioxidant Activities of Two Moroccan Thymus Essential Oils

Chemical Composition and Antimicrobial Activity of Essential Oil of Fruits from Vitex agnus-castus L., Growing in Two Regions in Bulgaria

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