Chemical Composition of Aqueous Ethanol Extract of <i>Luffa cylindrica</i> Leaves and Its Effect on Representation of Caspase‐8, Caspase‐3, and the Proliferation Marker Ki67 in Intrinsic Molecular Subtypes of Breast Cancer <i>in Vitro</i>

Abdelsalam, Ibrahim M.; Ashmawy, Abeer M.; Hilal, Amany; Eldahshan, Omayma A.; Ashour, Mahmoud

doi:10.1002/cbdv.201800045

Cited by 17 publications

(7 citation statements)

References 37 publications

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“…Another study reported the cytotoxicity of L. cylindrica against BT-474, MDA-MB-231, and HepG2 cells, with the IC50 value of 329.2 µ g/mL against HepG2 [31]. The major cytotoxic compounds were kaempferide, eriodictyol 7-O-glucoside, and apigenin 7-O-glucouronide ( Figure 3), which acted on caspases 3 and 8 [32].…”

Section: Araceaementioning

confidence: 98%

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

Mazumder

Biswas²,

Raja³

et al. 2020

Molecules

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Cancer or uncontrolled cell proliferation is a major health issue worldwide and is the second leading cause of deaths globally. The high mortality rate and toxicity associated with cancer chemotherapy or radiation therapy have encouraged the investigation of complementary and alternative treatment methods, such as plant-based drugs. Moreover, over 60% of the anti-cancer drugs are molecules derived from plants or their synthetic derivatives. Therefore, in the present review, an attempt has been made to summarize the cytotoxic plants available in the Indian subcontinent along with a description of their bio-active components. The review covers 99 plants of 57 families as well as over 110 isolated bioactive cytotoxic compounds, amongst which at least 20 are new compounds. Among the reported phytoconstituents, artemisinin, lupeol, curcumin, and quercetin are under clinical trials, while brazilin, catechin, ursolic acid, β-sitosterol, and myricetin are under pharmacokinetic development. However, for the remaining compounds, there is little or no information available. Therefore, further investigations are warranted on these subcontinent medicinal plants as an important source of novel cytotoxic agents.

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Section: Araceaementioning

confidence: 98%

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

Mazumder

Biswas²,

Raja³

et al. 2020

Molecules

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“…The IC50 of acetogenin-rich extract of avocado pulp against HEPG2 cells, MCF7 cells and HT29 cells was 8.1, 52.1 and 11.3 µg/ml, respectively. Cancer represents a serious health worldwide problem and its incidence is increased in Egypt and may reach three times starting from 2013 to 2050 [40]. Searching for new cytotoxic compounds is necessary, especially when come from natural sources.…”

Section: Table (2): Glc Analysis Of Hydrocarbons and Phytosterols Of Acetogenin-rich Extract Of Avocado Pulpmentioning

confidence: 99%

Antioxidant, anti-cancer and anti-arthritic activities of acetogenin-rich extract of avocado pulp

et al. 2021

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Avocado is an oil-rich fruit pleasant organoleptic characteristic, high nutritional value and health benefits due to its content of bioactive molecules. The objective of the current study was assessment of the antioxidant, anti-cancer and anti-arthritic activities of acetogenin-rich extract of avocado pulp. Total phenolic, total flavonoids, fatty acids profile, phytosterols and hydrocarbons of the extract were determined. Acetogenin was detected using GC-MS. The antioxidant activity of the extract was determined using DPPH, ABTS and FRAP assay. The cytotoxicity of the extract was assessed in different cancer cell lines (liver, colorectal and breast carcinoma cells). The anti-arthritic activity of the extract was evaluated in adjuvant induced arthritis (AIA) model in rats. The results revealed that acetogenin-rich extract of avocado contains phenolic compound (58.6±0.579 mg GAE/g extract) and total flavonoids (10.8±0.449 mg QE/g extract). GC-MS analysis revealed the presence of persediene as one of acetogenin compounds by 1.1% in the extract. The extract possessed antioxidant activity in all the studied methods and the maximum activity was against ABTS free radical. The IC50 of the extract against hepatocellular carcinoma cells (HEPG2), breast adenocarcinoma cells (MCF7) and colorectal cancer cells (HT29) was 8.1, 52.1 and 11.3 µg/ml, respectively. The extract showed significant anti-arthritic activity in rats through reduction of inflammation volume, improvement in the antioxidant status of arthritic rats as observed by reduction of plasma levels of malondiadehyde and elevation of the antioxidant enzymes (SOD, catalase and glutathione peroxidase) in association with reduction of the inflammatory marker TNF-α. The extract showed significant improvement in the histopatological studies of the joint tissue of arthritic rats. In conclusion: Acetogenin-rich extract of avocado pulp can be used as alternative treatment for prevention or treatment of cancer and rheumatoid arthritis due to its antioxidant and anti-inflammatory activities.

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“…Its fruit is also used to treat hyperglycemia [5,6], cataract [7], anti-emetic, anti-inflammatory [8], and antimicrobial effect. [9] Its leaves are also studied extensively on cancer [10], hepatoprotective [11], and anthelminthic [12] activities. Similarly, its seed also has the potential in treating asthma-related effects [13], anti-fungal [14], abortifacient [15], and anti-HIV activity [16].…”

Section: Introductionmentioning

confidence: 99%

The promising guide to LC–MS analysis and cholinesterase activity of Luffa cylindrica (L.) fruit using in vitro and in-silico analyses

Singh

Chitra

2023

Futur J Pharm Sci

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Background Luffa cylindrica (L.) is an annual climbing plant that produces fibrous fruit and can also be used as a vegetable in northern parts of India. Various studies have been carried out on the plant and found to have anti-inflammatory, antifungal, analgesic, anti-myocardial, anti-hyper triglyceride, immunostimulant, anti-allergic, and other properties. The ethanolic extract of the Luffa cylindrica (L.) fruit has not yet been subjected to LC–MS analysis for several bioactive chemicals that target neurological diseases. Oxidative stress is an inevitable situation in AD mechanisms and is a key bridge connecting various AD pathways. Results Luffa cylindrica contains various phytochemicals and showed highest alkaloid content of 21.39 ± 1.47 mg of AE/g. A total of 80 compounds were identified in the ethanolic extract from LC–MS analysis. The bioactive compounds were screened for eligibility by Lipinski's rule of five for docking with receptors responsible for causing oxidative stress-associated Alzheimer's disease. Perlolyrine was chosen to perform in-silico docking. An in vitro activity of cholinesterase showed highest inhibition at 500 µg/ml. In-silico docking of perlolyrine showed better binding affinity and score. Results revealed that out of 10 docked receptors, amyloid beta showed the highest binding affinity with an energy of − 46.1 kcal/mol showing promising drug for Alzheimer's disease. Conclusion Based on current findings, the study reports the presence of a promising, bioactive compound (perlolyrine) and in turn provides an optimistic note in exploring its biological activity in vivo with oxidative stress-related Alzheimer's disease.

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Chemical Composition of Aqueous Ethanol Extract of Luffa cylindrica Leaves and Its Effect on Representation of Caspase‐8, Caspase‐3, and the Proliferation Marker Ki67 in Intrinsic Molecular Subtypes of Breast Cancer in Vitro

Cited by 17 publications

References 37 publications

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

Antioxidant, anti-cancer and anti-arthritic activities of acetogenin-rich extract of avocado pulp

The promising guide to LC–MS analysis and cholinesterase activity of Luffa cylindrica (L.) fruit using in vitro and in-silico analyses

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