Chemical Composition, Insecticidal, Persistence and Detoxification Enzyme Inhibition Activities of Essential Oil of Artemisia maritima against the Pulse Beetle

Abstract

Pulse beetle is the major pests of pulses that cause significant loss to grains leads to unfit for consumption and marketing. Indiscriminate use of synthetic pesticides for the control of pulse beetle (Callosobruchus chinensis and Callosobruchus maculatus) led to insect resistance, pesticide residues on grains which affect consumer’s health and environment. Essential oils (EOs) are good alternatives to synthetics due to their safety to the environment and consumers’ health. The main objective of the present study was to explore the chemical composition, fumigant, repellency, ovipositional deterrence, persistence, and detoxification enzyme inhibition of Artemisia maritima essential oil against pulse beetle. Results showed that primary components of the EO were 1,8-Cineole and bornyl acetate. EO showed promising fumigant toxicity to C. chinensis and C. maculatus (LC50 = 1.17 and 0.56 mg/L, respectively) after 48 h. In the repellent assay, EO at 20 mg/L showed 92–96% repellence after 1 h. In ovipositional deterrence assay, EO showed more ovipositional deterrence against C. chinensis (OD50 = 3.30 mg/L) than C. maculatus (OD50 = 4.01 mg/L). Higher concentrations of oil (8 and 6 mg/L) in C. maculatus showed significant inhibition of the glutathione-S-transferase enzyme (7.14 and 5.61 n mol/min/mL, respectively).

Toxicity and synergistic activity of compounds from essential oils and their effect on detoxification enzymes against Planococcus lilacinus

“…(2021) , stating that T. minuta oil inhibited the AChE and GST activity against P. xylostella . Similarly, the essential oil from Artemisia maritima inhibited the GST activity against C. chinensis and C. maculatus , as reported previously ( Chauhan et al., 2022 ). However, pure compounds of monoterpenes such as α-bisabolol, 1-8-cineole, carvone, carvacrol, chamazulene, and limonene decreased the AChE and GST activities in insects ( Abdelgaleil et al., 2009 ; Rizvi et al., 2018 ).…”

“…Inhibition activities of detoxification enzymes [acetylcholinesterase (AChE) and glutathione-S-transferase (GST) were carried out using standard procedures ( Chauhan et al., 2022 ). Based on the toxicity assay report, four different concentrations of ocimene and β-myrcene (5,000, 2,500, 1,250, and 625 ppm) were prepared for detoxification enzyme inhibition activity.…”

Sublethal impacts of essential plant oils on biochemical and ecological parameters of the predatory mite Amblyseius swirskii

“…In contrast, GST, αand β-esterase activities were reduced by exposure to high concentrations of Cymbopogon flexuosus essential oil in Aedes aegypti L. (Diptera: Culicidae) (Carreño et al, 2018). Similarly, Liao et al (2017), Gao et al (2019), andChauhan et al (2022) reported inhibitory effects of essential oils on insect detoxifying enzymes. The Cymbopogon citratus essential oil significantly reduced detoxifying enzyme activity of larvae of Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae) (Moustafa et al, 2021).…”

Characterization of Triadica sebifera (L.) Small Extracts, Antifeedant Activities of Extracts, Fractions, Seed Oil and Isolated Compounds against Plutella xylostella (L.) and Their Effect on Detoxification Enzymes

“…Detoxification enzyme inhibition activities of leaf, bark extracts, and seed oil of T. sebifera against P. xylostella were performed as per the method followed [ 68 ]. Four different concentrations of leaf, bark extract, and seed oil (0.25, 0.5, 1, and 2%) were selected for enzyme activity assay according to the antifeedant assay described above.…”