Chemical composition, genotoxicity and antigenotoxicity study of Artemisia herba-alba using the eye and wing SMART assay of Drosophila melanogaster

Amkiss, Sanae; Dalouh, A.; Idaomar, Mohamed

doi:10.1016/j.arabjc.2020.102976

Cited by 14 publications

(7 citation statements)

References 47 publications

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“…For example, coadministration of ethanolic extract of Artemisia herba-alba (family: Asteraceae) with MMS showed a significant antimutagenic effect (P < 0.001) and the rate of inhibition was up to 84%. 64 Batista et al also showed that there was no genotoxic/mutagenic effect caused by Licania rigida (family: Chrysobalanaceae) leaf aqueous extract (5, 10 or 20 mg mL −1 ) compared to negative (treated with distilled water) and positive (treated with doxorubicin) controls of SMART. 65…”

Section: Smart Resultsmentioning

confidence: 99%

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Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

et al. 2022

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BACKGROUND Plants have important chemical compounds to protect organisms from many mutagens but they may also have dangerous toxic substances that must be tested before use. Lallemantia iberica has been used by local people for treatment of some diseases but it was never tested for its cell protective and antimutagenic/antirecombinogenic properties. In this study, it was aimed to determine the cytoprotective, antimutagenic/antirecombinogenic, antibacterial, antioxidant and phenolic profile of L. iberica by MTS assay, micronucleus test/somatic mutation recombination test (SMART), disc diffusion method, ABTS/DPPH tests and liquid chromatography (LC), respectively. RESULTS MTS assay results showed that both of the extracts supported the normal growth of healthy human lymphocytes and there was no significant difference between extracts. Although there was no significant increase in micronucleus concentration (‰) of the cultures treated with ethanol or methanol extracts, methanol extract caused slightly lower micronucleus concentration (12.64 ± 3.65‰) than the ethanol extract (24.46 ± 4.50‰). SMART results showed that ethanol and methanol extracts prevented harmful mitotic recombination. Lallemantia iberica also showed antibacterial activity against all of the strains tested, and the largest inhibition zones were observed with Pseudomonas aeruginosa (17.9 mm) and Enterobacter aerogenes (13.8 mm). Methanol extracts showed better antioxidant activities (ABTS IC50: 104.07 μg mL−1, DPPH IC50: 58.8 μg mL−1) than ethanol extracts (ABTS IC50: 344.03 μg mL−1, DPPH IC50: 233.7 μg mL−1). According to the LC results, the most abundant phenolic compounds were rutin hydrate (1796.4 ± 62.9 mg kg−1) and p‐coumaric acid (228.1 ± 7.60 mg kg−1). CONCLUSION Lallemantia iberica extracts had cell‐protective, antimutagenic/antirecombinogenic, antibacterial and strong antioxidant characteristics in relation to their rich phenolic content. © 2022 Society of Chemical Industry.

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Section: Smart Resultsmentioning

confidence: 99%

“…In addition, plants from different families also showed similar antimutagenic effects. For example, co‐administration of ethanolic extract of Artemisia herba‐alba (family: Asteraceae) with MMS showed a significant antimutagenic effect ( P < 0.001) and the rate of inhibition was up to 84% 64 . Batista et al .…”

Section: Resultsmentioning

confidence: 99%

Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

et al. 2022

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“…One study conducted by Hudaib and Aburjai (2006) reported that the main components of the essential oil from the aerial parts of AHA from Jordan were trans -sabinyl acetate (5.4%), germacrene D (4.6%), α-eudesmol (4.2%), and caryophyllene acetate (5.7%) ( Hudaib and Aburjai, 2006 ). Also, Amkiss et al (2021) showed that the major phytocomponents identified in AHA ethanolic extract using GC–MS were Anobin (37.30), a- Santonin (36.53), and Alkhanin (36.02). Moreover, another study done by Tilaoui et al (2011) showed that the major chemical composition of the essential oil of aerial parts of AHA was Bisabolone oxidea (17.55) and Farnesene epoxide, Ea (17.08).…”

Section: Discussionmentioning

confidence: 99%

Anti-MRSA and cytotoxic activities of different solvent extracts from Artemisia herba-alba grown in Shubak, Jordan

Alghonmeen,

Dmour,

Saghir

et al. 2024

Open Vet J

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Background: Globally, resistance to antimicrobial drugs is a major hazard to public health. Infections that were once easily treatable with antibiotics are becoming harder to control, leading to prolonged illnesses, increased mortality rates, and higher healthcare costs. Aim: This study intended to assess the antimicrobial, specifically the anti- Methicillin-resistant Staphylococcus aureus (MRSA) , and anticancer properties of different extracts obtained from A. herba-alba (AHA). Methods: The antibacterial tests of AHA were performed on two Gram-negative bacterial strains (Escherichia coli and Klebsiella pneumonia), two Gram-positive bacterial strains (Methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus). Initial screening for antibacterial activities was conducted using the agar-disc diffusion technique. Subsequently, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined through the broth-dilution assay. The anticancer test was carried out in vitro on a human colorectal carcinoma cell line (HCT-116) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) )MTT( method. Results: Among all extracts, n-hexane extract of AHA was the most effective against S. aureus with the highest inhibition zone (24.67 mm) comparing to standard antibiotic (erythromycin, 24.00 mm) followed by the methanolic extract against MRSA (24.00 mm). The methanol extract of AHA showed the highest antibacterial activity against MRSA. The results of MIC and MBC of the AHA methanol extract against MRSA were 1.17±1.09 mg/mL and 9.375±0.0 mg/mL, respectively demonstrated therapeutically significant antibacterial activity. Ethyl acetate extract has no antibacterial activity against E. coli and K. pneumonia. The findings indicated that the methanol extract of AHA exhibited the highest efficacy against the colorectal carcinoma cell line (HCT-116), with an IC50 value of 126.61±13.35 µg/mL. Conclusion: These findings suggest that methanol extract of AHA could be considered as a potential agent to serve as a source of antibacterial and anticancer compounds.

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“…The genotoxic activity evaluation of natural products, and especially of medicinal plants, has become into something essential in order to guarantee long-term risks absence ( Amkiss et al, 2021 ). With SMART methodology, more than 250 compounds have been analyzed ( Vogel et al, 1985 , Vogel, 1992 , Vogel and Nivard, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

In vivo evaluation of the toxic activity and genotoxicity of the Hymenaea courbaril L.’s resin in Drosophila melanogaster

Anaya-Gil

Ramos-Morales

Muñoz-Hernández

et al. 2022

Saudi Journal of Biological Sciences

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Due to the negative consequences carried by the usage of synthetic insecticides, a global interest into finding substitutes for these chemical compounds through natural products has arisen. When yielded to external attacks, plants generally produce metabolites to defend themselves. The physicochemical characteristics of this kind of compounds have allowed their usage as potential bioinsecticides. The Hymenaea courbaril L. (algarrobo) has proven to be a plant rich in metabolites with outstanding biological activity, in such a way that some of its extracts have been tested as insecticides. The goal of this study was to know the phytochemical composition of Hymenaea courbaril L.’s resin and perform evaluations in vivo of its toxic and genotoxic effects in the biological model Drosophila melanogaster . For this, two resin extracts were prepared and both a phytochemical analysis were carried out on them, having found in the ethanolic total extract the presence of terpenes, flavonoids and coumarins, while in the partial ethanolic extract only presence of terpenes and flavonoids was found. Drosophila larvae were submitted to different concentrations of the extracts and both the survival and the sexual ratio were evaluated, finding that larvae are more sensitive to the partial ethanolic extract. Subsequently, the induction of somatic mutation and mitotic recombination (SMART) was evaluated in the flies’ eyes. The most significant affectations at a genotoxic level were found when larvae were tested with the partial extract, indicating that possibly the coumarins absence makes this insect more susceptible to damages at a genetic material level.

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Chemical composition, genotoxicity and antigenotoxicity study of Artemisia herba-alba using the eye and wing SMART assay of Drosophila melanogaster

Cited by 14 publications

References 47 publications

Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

Anti-MRSA and cytotoxic activities of different solvent extracts from Artemisia herba-alba grown in Shubak, Jordan

In vivo evaluation of the toxic activity and genotoxicity of the Hymenaea courbaril L.’s resin in Drosophila melanogaster

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