Chemical composition, cytotoxicity and antimicrobial activity of selected plant‐derived essential oils against fish pathogens

Assane, Inácio Mateus; Valladão, Gustavo Moraes Ramos; Pilarski, Fabiana

doi:10.1111/are.14935

Cited by 14 publications

(4 citation statements)

References 72 publications

(82 reference statements)

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“…The cytotoxicity and antibacterial effects of several herbs have been previously examined [34,35], highlighting their potential use for controlling bacterial diseases in cultured fish. In our study, CEO inhibited bacterial growth, both in saline and ethanolic solutions.…”

Section: Antimicrobial Activity Of Ceomentioning

confidence: 99%

Antimicrobial and Toxic Activity of Citronella Essential Oil (Cymbopogon nardus), and Its Effect on the Growth and Metabolism of Gilthead Seabream (Sparus aurata L.)

García‐Márquez

Barany

Ruiz

et al. 2021

Fishes

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Aquaculture procedures usually induce stress that affects the physiological status of fish. For this reason, the inclusion of additives in fish feeds to palliate stress might be a good alternative. This study aimed to assess the antimicrobial activity of citronella (Cymbopogon nardus) essential oil (CEO) against bacterial pathogens and to determine its dietary impact on the growth performance of Sparus aurata. In vitro tests confirmed that CEO possesses antimicrobial activity against several fish-specific pathogens. For the in vivo tests, three experimental groups were fed for 60 days with different concentrations of CEO: CTRL (0 mL kg−1 fish feed); CEO1 (1 mL kg−1 fish feed); and CEO2 (2 mL kg−1 fish feed). At the end of the experiment, the physiological status was characterized. Subsequently, the specimens of the CTRL and CEO2 groups were subjected to a challenge with an injection of Poly I:C for immune stimulation. Although S. aurata individuals tolerated CEO inclusion without compromising growth performance, it significantly reduced glycogen in the CEO2 group, concomitant to an increment of total peripheral leucocytes. Moreover, different hematological profiles’ responsive patterns against an inflammatory stimulus were observed. In conclusion, our results suggest that the use of CEO as a fish feed additive can prevent bacterial outbreaks and improve potential in vivo disease resistance in S. aurata without negatively affecting growth.

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Section: Antimicrobial Activity Of Ceomentioning

confidence: 99%

Antimicrobial and Toxic Activity of Citronella Essential Oil (Cymbopogon nardus), and Its Effect on the Growth and Metabolism of Gilthead Seabream (Sparus aurata L.)

García‐Márquez

Barany

Ruiz

et al. 2021

Fishes

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“…EO, a variety of plant EOs exhibited the ability to inhibit the growth of A. hydrophila ( Iturriaga et al, 2012 ; Ruiz-Navajas et al, 2012 ; Majolo et al, 2017 ). For example, Thymus vulgaris , Eugenia caryophyllus , and Tee Tree EO inhibited the growth of A. hydrophila ( Assane et al, 2021 ). Screening of EOs of different plant-derived varieties indicated that 14 of them were found to be active against A. hydrophila ( Kot et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Essential Oils From Citrus unshiu Marc. Effectively Kill Aeromonas hydrophila by Destroying Cell Membrane Integrity, Influencing Cell Potential, and Leaking Intracellular Substances

Zhong

Chen²,

Yang³

et al. 2022

Front. Microbiol.

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Aeromonas hydrophila is one of the important pathogenic bacteria in aquaculture causing serious losses every year. Essential oils are usually used as natural antimicrobial agents to reduce or replace the use of antibiotics. The aim of this study was to evaluate the antibacterial activity and explore the mechanisms of essential oil from satsuma mandarin (Citrus unshiu Marc.) (SMEO) against A. hydrophila. The results of the gas chromatography-mass spectrometer demonstrated that SMEO contains 79 chemical components with the highest proportion of limonene (70.22%). SMEO exhibited strong antibacterial activity against A. hydrophila in vitro, the diameter of the inhibition zone was 31.22 ± 0.46 mm, and the MIC and MBC values were all 1% (v/v). Intracellular material release, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and flow cytometry analysis revealed the dynamic antibacterial process of SMEO, the morphological changes of bacterial cells, and the leakage process of intracellular components. These results demonstrated that SMEO disrupted the extracellular membrane permeability. Our study demonstrated that SEMO has the potential to be used to control and prevent A. hydrophila infections in aquaculture.

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“…The in vitro cytotoxic effect of six synthetized HDPs, FFC, OTC and TAP against fish RBCs was studied by examining the haemolytic activity of each antimicrobial against Nile tilapia Oreochromis niloticus RBCs following the method described in our study (Assane et al . 2021). Briefly, fresh blood was collected from healthy juvenile Nile tilapia by punction of caudal blood vessels, then washed three times by centrifugation (500 g for 5 min, 4°C) with phosphate‐buffered saline (PBS, pH 7·4).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity and antimicrobial activity of synthetic peptides alone or in combination with conventional antimicrobials against fish pathogenic bacteria

et al. 2021

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Aims This study aimed to evaluate the in vitro cytotoxicity and efficacy of synthetic host defence peptides (HDPs), alone or in combination with florfenicol (FFC), oxytetracycline (OTC) or thiamphenicol (TAP), against different pathogenic bacteria isolated from diseased fish. Methods and Results Solid‐phase synthesis, purification and characterization of several HDPs were performed manually, using the fluorenylmethyloxycarbonyl protecting group in different resins and via high‐performance liquid chromatography‐mass spectrometry, respectively. The in vitro cytotoxicity and antimicrobial activity of HDPs, FFC, OTC and TAP against Nile tilapia red blood cells (RBCs) and relevant fish pathogenic bacteria (Aeromonas, Citrobacter, Edwardsiella, Streptococcus, Lactococcus and Vibrio) was determined using the haemolysis assay and broth microdilution method, respectively. The checkerboard assay was used to evaluate the synergy between the most active HDPs and other antimicrobials against the tested strains. MUC 7 12‐mer, FFC, OTC and TAP were not cytotoxic to Nile tilapia RBCs, in all tested concentrations. LL‐37, (p‐BthTX‐I)2 and Hylin‐a1 were not cytotoxic at concentrations up to 78·13, 19·53 and 9·77 μg ml−1, respectively. HDPs demonstrated potent antimicrobial activity (minimum inhibitory concentration ≤31·25 µg ml−1) against Aeromonas jandaei (KR‐12‐a5), Citrobacter freundii (Kr‐12‐a5; (p‐BthTX‐I)2; LL‐37; and Hylin a1), Streptococcus agalactiae (Hylin a1; (p‐BthTX‐I)2 and LL‐37), Lactococcus garviae (Hylin a1), and Vibrio fluvialis (KR‐12‐a5). The combinations of (p‐BthTX‐I)2 with TAP and LL‐37 with FFC showed synergistic activity against C. freundii (fractional inhibitory concentration index of 0·25 and 0·50, respectively). Conclusions Synthetic HDPs have the potential as a good treatment option for bacterial diseases in aquaculture. Significance and Impact of the Study The in vivo effectiveness of synthetic HDPs such as KR‐12‐a5; LL‐37; (p‐BthTX‐I)2 and Hylin a1 can be tested alone or in combination with conventional antimicrobials as a treatment option to reduce the use of antimicrobials in aquaculture.

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Chemical composition, cytotoxicity and antimicrobial activity of selected plant‐derived essential oils against fish pathogens

Cited by 14 publications

References 72 publications

Antimicrobial and Toxic Activity of Citronella Essential Oil (Cymbopogon nardus), and Its Effect on the Growth and Metabolism of Gilthead Seabream (Sparus aurata L.)

Antimicrobial and Toxic Activity of Citronella Essential Oil (Cymbopogon nardus), and Its Effect on the Growth and Metabolism of Gilthead Seabream (Sparus aurata L.)

Essential Oils From Citrus unshiu Marc. Effectively Kill Aeromonas hydrophila by Destroying Cell Membrane Integrity, Influencing Cell Potential, and Leaking Intracellular Substances

Cytotoxicity and antimicrobial activity of synthetic peptides alone or in combination with conventional antimicrobials against fish pathogenic bacteria

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