Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus

Kalogeropoulos, Nick; Konteles, Spyros J.; Troullidou, Elena; Mourtzinos, Ioannis; Karathanos, Vaios Τ.

doi:10.1016/j.foodchem.2009.02.060

Cited by 289 publications

(225 citation statements)

References 34 publications

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“…The main classes of compounds identified and their abundances are listed in Table 2. GC-MS analysis was chosen for chemical profiling because of the high resolving power of capillary GC and the valuable structural information provided by the EIMS, especially for phenolics and terpenes (Cuesta-Rubio et al, 2007;Kalogeropoulos, Konteles, Troullidou, Mourtzinos, & Karathanos, 2009;Popova et al, 2010), despite the disadvantages of the silylation procedure.…”

Section: Resultsmentioning

confidence: 99%

The specific chemical profile of Mediterranean propolis from Malta

et al. 2011

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a b s t r a c tSeventeen Maltese propolis samples were studied by GC-MS after silylation. They exhibited the typical Mediterranean chemical profile, rich in diterpene compounds (18-92% of TIC, GC-MS): 32 individual diterpenes were identified; 22 of them were present in each specimen. The other abundant compound group was that of sugars and sugar derivatives. In some samples, however, another compound group was observed (0-12% of TIC, GC-MS); the corresponding mass spectra were consistent with monoand sesquiterpenyl esters of substituted benzoic acids. Two new propolis constituents of this group, daucane diterpene esters of hydroxybenzoic acids, were isolated. Their origin is suggested to be Ferula communis, as they are taxonomic markers for this species. All propolis samples were active against Staphylococcus aureus but only those with high concentrations of terpenyl esters showed antifungal activity against Candida albicans. The present results confirm that Mediterranean propolis is a valuable natural product with potential to improve human health.

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Section: Resultsmentioning

confidence: 99%

The specific chemical profile of Mediterranean propolis from Malta

et al. 2011

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“…[2][3][4][5][6] As propolis has characteristics that are beneficial to human health, such as antioxidant, anti-inflammatory, antifungal and anticancer activities, its chemical composition and properties have been extensively studied. [7][8][9][10] The antioxidant activity of ethanolic extracts of propolis has been evaluated by free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH • ) scavenging, 8 by the ferric reducing ability power (FRAP) using the ferric-tripyridyltriazine (Fe 3+ -TPTZ) complex 11 and by oxidation of linoleic acid. 5 Similarly, mass spectrometry analyses has been used to examine the chemical composition of propolis extracts and its correlation with type and properties.…”

Section: Introductionmentioning

confidence: 99%

“…9,12 The compositions of total phenolic compounds by FolinCiocalteu method and of flavonoid by using AlCl 3 have been determined. 11,13 It seems, however, that the correlation between the antioxidant activity and the contents of phenolic compounds and flavonoids of propolis extracts obtained with different ethanol contents and propolis concentrations has not been investigated yet.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity and composition of propolis obtained by different methods of extraction

Cottica¹,

Sawaya²,

Eberlin³

et al. 2011

J. Braz. Chem. Soc.

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Extratos etanólicos de própolis obtidos a partir de diferentes concentrações de própolis e com álcool hidratado com diferentes teores de álcool/água foram analisados. A ação antioxidante destes extratos foi determinada através da captura do radical livre 1,1-difenil-2-picril-hidrazila (DPPH•) e do poder de redução férrico (FRAP) utilizando o complexo férrico-tripiridiltriazina (Fe 3+ -TPTZ). O teor de compostos fenólicos totais e a quantificação de flavonóides também foram verificados e obtidos por espectrometria de massas com ionização por electrospray (ESI(-)-MS) dos extratos. Observou-se que a composição química dos extratos de própolis e sua atividade antioxidante variam com a concentração de própolis e, principalmente, com o teor de álcool/água do etanol hidratado utilizado na extração. Foi possível perceber também que a atividade antioxidante dos extratos é significativamente dependente destes parâmetros de extração e que as composições de compostos fenólicos e flavonóides também variam muito. Os valores de FRAP não apresentaram, porém, boa correlação com o teor de flavonóides.Ethanolic extracts of propolis obtained with different concentrations of propolis and made using hydrous ethanol with various water/ethanol contents were analyzed. The antioxidant action of the extracts was determined by free radical 1,1-diphenyl-2-picryl-hydrazyl (DPPH•) scavenging and by ferric reducing ability power (FRAP), using the ferric-tripyridyl triazine (Fe 3+ -TPTZ) complex. The total phenolic and flavonoid contents were also determined, and the electrospray ionization mass spectrometry spectra (ESI(-)-MS) of the extracts were acquired. The chemical composition of propolis extracts and their antioxidant activity were observed to vary with the concentration of propolis, and especially with the water/ethanol contents of the hydrous ethanol used for extraction. The antioxidant activity of the extracts was also found to be significantly dependent on these extraction parameters, as well as on the composition of the phenolic compounds and flavonoids. FRAP values showed, however, poor correlation with the flavonoid content. Keywords: total phenolic contents, antioxidant activity, ESI(-)-MS fingerprint, propolis extraction, flavonoids IntroductionPropolis is a resin produced by honey bees by mixing wax, pollen, salivary secretions and collected natural resins. 1 The chemical composition of propolis is complex and varies with the vegetation visited by bees, as well as with bee species. [2][3][4][5][6] As propolis has characteristics that are beneficial to human health, such as antioxidant, anti-inflammatory, antifungal and anticancer activities, its chemical composition and properties have been extensively studied. [7][8][9][10] The antioxidant activity of ethanolic extracts of propolis has been evaluated by free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH • ) scavenging, 8 by the ferric reducing ability power (FRAP) using the ferric-tripyridyltriazine Antioxidant Activity and Composition of Propolis Obtained by Different Methods o...

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“…Owing the ability to synthesize many different substances, the propolis is one of the top richest sources of new drugs (1,11). It is showed that ethanol extract of propolis has a high potential as an alternative source of antibacterial compounds (2,3,4,5,13,19,20,22,33).…”

Section: Introductionmentioning

confidence: 99%

In vitro antibacterial activities of ethanol extract of iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri & Streptococcus iniae)

Tukmechi¹,

Ownagh²,

Mohebbat³

2010

Braz. J. Microbiol.

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The ''in vitro'' antibacterial activity of ethanol extract of propolis (EEIP) from Urmia, Iran was investigated against three prevalent species of fish bacterial pathogens including: Aeromonas hydrophila LMG 3770, Yersinia ruckeri LMG 3279 and Streptococcus iniae LMG 14520. In this study two standard susceptibility testing techniques (Micro-broth dilution method and Agar-well diffusion method) were used to evaluation of the antibacterial activity of EEIP against the mentioned micro-organisms. Also the chemical composition of propolis was determined by the method of Gas chromatography-mass spectrometry (GC-MS). Twenty-six compounds were identified by gas chromatography-mass spectrometry analysis. Results showed Chemical composition of EEIP contained significant amounts of flavonoids, Sesquiterpenes -mainly Eudesmol and Caryophyllene oxide -aromatic acid, and low amounts of aldehydes and triterpens. Furthermore the ethanol extract of propolis inhibited the growth of all examined micro-organisms with the highest antimicrobial activity against Gram-positive bacteria Streptococcus iniae. Ethanol did not influence the antimicrobial effect of EEIP. These antibacterial properties would warrant further studies on the clinical applications of propolis in aquaculture field.

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Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus

Cited by 289 publications

References 34 publications

The specific chemical profile of Mediterranean propolis from Malta

The specific chemical profile of Mediterranean propolis from Malta

Antioxidant activity and composition of propolis obtained by different methods of extraction

In vitro antibacterial activities of ethanol extract of iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri & Streptococcus iniae)

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