Chemical composition, antioxidant activity and cytotoxicity of flower extracts from Magnolia grandiflora L. found in southeast Mexico

Abstract

Many reports state that different structures of M. grandiflora contain bioactive components. Nonetheless, phytochemical studies reported that the extracted essential oils are chemically different and remarkably variable in their qualitative and quantitative compositions. Further studies Mexican M. grandiflora needs to be done. Thus, the research aimed to characterize a) the chemical composition, b) the antioxidant activity and c) cytotoxicity effect of two M. grandiflora flower extracts. The chemical composition was evaluated by preliminary phytochemical test followed by thin layer chromatography, UV-Vis and FTIR spectrophotometer analysis. The antioxidant activity of the flower extracts was measured by the free radical-scavenging activity (ABTS) and the stable radical of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method and the cytotoxicity by an Artemia salina bioassay. Water and ethyl flower extracts showed the presence of organic chromophores such as flavonoids. Both extracts (ethyl and water) demonstrated antioxidant activity by both ABTS (459.6±8.5 µmol TE g-1 and 274.2±5.7 µmol TE g-1 respectively) and DPPH (3210.4 ± 2.5 µmol TE g-1 and 219.7 ± 0.9 µmol TE g-1 respectively) methologies; and non-cytotoxic activity (LC50 , µg mL-1) (1,285 ± 14 and 1,116 ± 15 respectively). The water and ethyl extracts of M. grandiflora flowers found in southeast Mexico are a promissory source of chemical compounds with attributed biological activity according to the presented results.

Phytochemical Analysis and Specific Activities of Bark and Flower Extracts from Four Magnolia Plant Species

“…Studies reported polyphenols levels, ranging from 14 mg/g to 17 mg/g, between 86 mg GAE/g and 192 mg GAE/g, at 46.8 mg GAE/g, with variations depending on factors like moisture and temperature. The antioxidant activity in these studies recorded to range between 84 and 311 mg VCE/g MFE, or was 54.5µg/mL (DPPH/IC50), 459.6µmol TE/g (ABTS), thus demonstrating the significant antioxidant potential of Magnolia species [21][22][23].…”