Chemical composition, antibacterial and antifungal properties of Tunisian Nigella sativa fixed oil

Harzallah, Hanene Jrah; Noumi, Emira; Békir, Karima; Bakhrouf, Amina; Mahjoub, Touhami

doi:10.5897/ajmr11.1073

Cited by 9 publications

(7 citation statements)

References 17 publications

(18 reference statements)

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“…Both samples gave more or less the same percentage yield of fixed oil (EFO = 38.25% and IFO =38.05%) (Table 1). These amounts were higher than those reported for fixed oil obtained from seeds grown in Turkey (24.4-29.5 % (Nimet et al, 2015)) and Iran ((30-35 %) (Harzallah et al, 2012;Hosseinia et al, 2019)). They were comparable to yields reported from seeds grown in Yemen (36.8-38.4% (Al-Naqeeb et al, 2009) and Morocco (37%, (Gharby et al, 2015).…”

Section: Chemical Profile Of Oils Samplescontrasting

confidence: 68%

“…The amount of linoleic acid was higher in IFO while that of oleic acid was relatively higher in the EFO. Linoleic acid content was comparable or slightly higher than recorded from Iranian (51.67% and 55.95%) (Hosseinia et al, 2019) and Saudi-Arabia (56.5%) (Gharby et al, 2015) seeds samples and lower than that of Tunisian origin (58%) (Harzallah et al, 2012). Hydrodistillation of N. sativa seeds of Ethiopian origin (EEO) resulted in a yield of 9.2% dark brown-coloured essential oil (Table 1).…”

Section: Chemical Profile Of Oils Samplesmentioning

confidence: 71%

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Comparative chemical profiling and antimicrobial activity of Nigella sativa seeds oils obtained from different sources

Fatima¹,

Sonibare²,

Yagi³

2022

NRFHH

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Nigella sativa L. seeds are widely used in Sudan as a spice, food preservative, and medicine. Sudan does not grow the plant. The study aimed to compare the chemical profile and antibacterial activity of fixed and essential oils of N. sativa from Ethiopian and Indian seeds and the oil offered by Attarin in the local market. A Soxhlet device extracted fixed oils and hydrodistillation to obtain essential oils and analysed their oil profile using GC-MS. Disk diffusion was used to test antimicrobial activity. The fixed oil of Ethiopian (EFO) and Indian (IFO) seeds contained ten components, with linoleic acid (50.12% in EFO and 57.69% in IFO) being the most abundant. Ethiopian seeds were used to extract the essential oil. 51.96% of the oil was hydrogenated monoterpenes. The main chemicals were p-cymene (36.76%) and thymoquinone (18.70%). There were fixed and essential oils in the Attarin oil sample. The main component was linoleic acid (14.61%), followed by p-cymene (13.85%). The maximum antibacterial activity (MIC 6.25 µg/disc) was seen in both fixed and liquid oil samples against Escherichia coli and Pseudomonas aeruginosa. The best anti-P. aeruginosa action was attarin oil (MIC 12.5 µg/disc). Finally, the Sudanese market needs to standardise N. sativa seeds and oil.

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Section: Chemical Profile Of Oils Samplescontrasting

confidence: 68%

Section: Chemical Profile Of Oils Samplesmentioning

confidence: 71%

Comparative chemical profiling and antimicrobial activity of Nigella sativa seeds oils obtained from different sources

Fatima¹,

Sonibare²,

Yagi³

2022

NRFHH

View full text Add to dashboard Cite

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“…However, lowering of extraction temperature and time periods eliminates its biological activity on Serratia marcescens bacteria. The results shows powerful effects of Nigella sativa seeds watery extract on bacteria without reach a drastic or complex biochemical extraction process [27][28][29]. The UV-VIS spectrum reported an increase of extracted compounds with increased temperature.…”

Section: Resultsmentioning

confidence: 99%

Investigating the Efficiency of Simple Aqueous Extract of Nigella Sativa Activity Against Serratia Marcescens Bactria

Shkhaier

Majid

Alawi

et al. 2020

MJS

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Nigella sativa seeds is considered as a very effective treatment for a variety of human diseases in traditional medicine worldwide with low human toxicity. Serratia marcescens pathogenic species, a Gram-negative, opportunistic bacteria, are widespread and distributed in the human environment with considered resistance to different types of antibiotics. Extraction was performed on 12.5 gm. /150 ml of Nigella seeds using rotary evaporator. Different time periods for extraction were applied (1-4 hours) with different temperature ranges of 50 °C to 60 °C performed separately for each time period. The 3 and 4 hours extraction period at 60 °C for seeds was effective against Serratia marcescens with inhibition zone of 11.18 and 12.27 mm respectively. Nigella seeds could be a promising inhibitor with enhanced activity against Serratia marcescens. Water extraction was efficient and effective at 60 °C, with extraction period of 2-3 hours for seeds. No activity recorded below this temperature.

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“…innocua and E . coli were, respectively, 7 mm and 4.66 mm [ 43 ]. The antimicrobial activity of fractions (SH4) and (SH7) relied on compounds 17 pentatriacontene (50%) and hexadecane which confirmed their bioactivity [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition Related to Antimicrobial Activity of Moroccan Nigella sativa L. Extracts and Isolated Fractions

Tiji

Rokni

Benayad

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Nigella sativa L. (NS) is an aromatic and medicinal plant commonly used in Mediterranean cuisine. Its grains contain a large amount of fixed oil and have many therapeutic virtues and medicinal properties (antioxidant, antidiabetic, antimicrobial, and anticancer). Aim. The aim of this work is to study the antimicrobial activity of Nigella sativa L. extracts and separated fractions on various pathogenic strains and to correlate that with its chemical composition. Methods. Extracts from Moroccan Nigella sativa seeds were extracted using successive organic solvents, and their hexane and acetone extracts were separated by column chromatography. The chemical composition of extracts, fractions, and essential oil was determined by GC-MS and HPLC-DAD. Extracts and fractions were evaluated for antimicrobial activity through disk diffusion against Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, and Listeria innocua), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Candida pelliculosa) for 1 mg/mL concentration. Bacterial strains were followed to study their behaviors over time in different concentrations. The minimum inhibitory concentration of Nigella sativa essential oil was determined against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Candida albicans. Results and Conclusion. Although hexane extract was active against both types of bacteria (Gram+ and Gram−), some of its fractions were specifically active against only one type. Fraction (SH4) had the highest activity (15 mm inhibitory diameter). Acetone extract was nonactive but surprisingly resulted in specific active fractions, and the most interesting one was (SA7) that had an inhibitory diameter of 13 mm. This antibacterial effect was related to fatty acids (linoleic and palmitic acids) in (SH4) and 17 pentatriacontene in (SA7). Moreover, the antifungal activity of hexane fractions (10–13 mm) was higher than hexane extract (8 mm), but for acetone, it was the opposite. Acetone extract had a higher activity (18 mm) than its fractions (8–12 mm), except for (SA7) (19 mm). Those inhibitions were attributed to gallic acid, cysteine, and apigenin in acetone extract and cysteine with ascorbic acid in fraction (SA7). Antifungal activity of the essential oil was more pronounced than the antibacterial one. Indeed, determined MICs in the first case were on the microgram scale (MIC = 8 μg/mL, Candida albicans), while in the second case, they were on the milligram scale (MIC = 0.96 mg/mL for Staphylococcus aureus, 0.5 mg/mL for Bacillus cereus, and 0.68 mg/mL for Escherichia coli). This antifungal activity was attributed to three major compounds beta-cymene, alpha-thujene, origanene, and thymoquinone. Results of strains behavior over time at different concentrations of the fractions showed all the curves went through a maximum around 20 hours and had a delay of expression of 5 hours at the start. Taking all results into count, Nigella sativa L. extracts and/or derived principles could form promising antimicrobial agents for therapeutical and industrial uses.

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Chemical composition, antibacterial and antifungal properties of Tunisian Nigella sativa fixed oil

Cited by 9 publications

References 17 publications

Comparative chemical profiling and antimicrobial activity of <i>Nigella sativa</i> seeds oils obtained from different sources

Comparative chemical profiling and antimicrobial activity of <i>Nigella sativa</i> seeds oils obtained from different sources

Investigating the Efficiency of Simple Aqueous Extract of Nigella Sativa Activity Against Serratia Marcescens Bactria

Chemical Composition Related to Antimicrobial Activity of Moroccan Nigella sativa L. Extracts and Isolated Fractions

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