Chemical composition and In vitro antioxidant and antidiabetic activities of Eucalyptus Camaldulensis Dehnh. essential oil

Abstract

The present study was designed to determine the composition of the essential oil of Eucalyptus camaldulensis Dehnh. leaves and to examine its in vitro antioxidant and antidiabetic activities. The chemical composition of the essential oil from Eucalyptus camaldulensis Dehnh. leaves was analyzed by GC/GC-MS, twenty-nine compounds representing 99.10% of the total oil were identified. The major components of the oil were p-cymene (68.43%), 1,8-cineole (13.92%), 1-(S)-Į-pinene (3.45%) and R-(+)-limonene (2.84%). The antioxidant features of the essential oil were evaluated using inhibition of 2,2-diphenyl-1-picrylhydrazyl, hydroxyl, and superoxide radicals, inhibition of hydrogen peroxide and lipid peroxidation assays. We also studied Į-amylase and Į-glucosidase inhibition in vitro to assess the antidiabetic properties of the essential oil. Both Į-amylase and Į-glucosidase were inhibited by a non-competitive mechanism.

The Effects of Curcumin on Alpha Amylase in Diabetics Rats

“…One of the therapeutic approaches to lower postprandial blood glucose is to inhibition breakdown of starch by inhibiting carbohydrate hydrolysis enzymes such as alpha amylase and alpha glucosidase. For this reason, efforts to develop new pharmacological agents focus on the inhibition these enzymes [23,24]. Alpha-amylase catalyzes the hydrolysis of α-(1, 4)-D-glycosidic linkages of starch and other glucose polymers.…”

Exploration of natural enzyme inhibitors with hypoglycemic potentials amongst Eucalyptus Spp. by in vitro assays

“…have been used in folk medicine for the treatment of ailments such as colds, fever, toothache, diarrhea and snake bites. Uses of Eucalyptus leaf hot decoctions as "herbal tea" have been recorded in Aboriginal, European and British Pharmacopeias for the traditional remedy of type DM [10][11][12][13][14][15][16][17][18][19][20][21] . A rich literature exists, reporting over 500 Eucalyptus species with different pharmacological actions [11][12][13][14][15][16][17][18][19][20][21][22] .…”

“…Uses of Eucalyptus leaf hot decoctions as "herbal tea" have been recorded in Aboriginal, European and British Pharmacopeias for the traditional remedy of type DM [10][11][12][13][14][15][16][17][18][19][20][21] . A rich literature exists, reporting over 500 Eucalyptus species with different pharmacological actions [11][12][13][14][15][16][17][18][19][20][21][22] . Hypoglycemic potentials of Eucalyptuses are documented, but the mechanistic actions need to be explored further [11][12][13][14][15][16][17][18][19][20][21] .…”

“…A rich literature exists, reporting over 500 Eucalyptus species with different pharmacological actions [11][12][13][14][15][16][17][18][19][20][21][22] . Hypoglycemic potentials of Eucalyptuses are documented, but the mechanistic actions need to be explored further [11][12][13][14][15][16][17][18][19][20][21] . Inhibiting the actions of carbohydrate hydrolyzing enzymes like α-amylase and α-glucosidase helps to reduce post-prandial (PP) hyperglycemia.…”

“…Attenuation in ROS level may be due to increased production or diminished depletion of enzymes, like catalase, glutathione peroxidase and superoxide dismutase. Natural antioxidants which scavenge free radicals can provide a synergistic action to the overall antidiabetic potential of a plant [12,13] . Namiki (1981, 1985) reported the presence of a powerful antioxidant, 4-hydroxytritriacontane-16,18 dione, in the leaf wax of different Eucalyptus species [24,25] .…”