Chemical composition and in vitro evaluation of antioxidant, antimicrobial, and enzyme inhibitory activities of Erucaria uncata and Thymeleae hirsuta

Djermane, Nadia; Gali, Lynda; Arhab, Rabah; Gherraf, Noureddine; Bensouici, Chawki; Erenler, Ram­azan; Gok, Mesut; Abdessamed, Amina

doi:10.1016/j.bcab.2020.101834

Cited by 8 publications

(11 citation statements)

References 51 publications

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“…The aqueous extract from leaves also showed some activity in the antihyaluronidase assay. Our results, therefore, not only confirm previous reports from Djeridane et al, 10 Trigui et al, 15 and Djermane et al 27 on the antioxidant properties of T. hirsuta and a possible link with phenolic compounds through hydrogen transfer reactions but also extend the spectrum of activity of this plant since in addition to antiacetylcholinesterase, antibutylcholinesterase, and anti-α-glucosidase activities, 27 our experiments also showed an inhibitory activity against some skin enzymes, especially l -DOPA tyrosinase and elastase. Nowadays, there is an increasing demand for the use of natural compounds for health and beauty, and the experimental evidence of these inhibitory activities are potentially interesting in cosmetics for maintenance of hydration and repair of the skin.…”

Section: Resultssupporting

confidence: 92%

“…Thus, the antioxidant activity observed could be related to these polyphenols, as already reported in the literature. 27,28 In the flower methanolic extract only, 3 main peaks (Rf respectively of 19.5, 23.5, and 27.5 minutes) could be detected in the HPLC-UV-ELSD analysis and at 330 nm (Supplemental Figure S1). Thus, because of its high activity and the availability of a sufficient amount of this extract compared with the leaf extract, the bioguided fractionation was conducted using the flower extract.…”

Section: Resultsmentioning

confidence: 99%

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Antifungal and Antiaging Evaluation of Aerial Part Extracts ofThymelaea hirsuta(L.) Endl

Amari

Razafimandimby²,

Auberon

et al. 2021

Natural Product Communications

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In western Algeria, Thymelaea hirsuta aerial parts are traditionally used topically for the treatment of human skin infections. For a better knowledge of its biological activities, this study aimed at evaluating the in vitro antifungal activity and antiaging properties of several extracts of the aerial parts by in vitro assays. Antifungal activity was investigated by using the reference microbroth dilution method (National Committee for Clinical Laboratory Standards) against the dermatophytes ( Microsporum audouinii, Nannizzia gypsea, Trichophyton interdigitale, and Trichophyton rubrum) and the yeast ( Candida albicans). Antiaging (inhibition of 1,1-diphenyl-2-picrylhyorazyl radical scavenging and tyrosinase, lipoxygenase, elastase, and hyaluronidase activities) assays were performed in 96-well plates. All the extracts of T. hirsuta exhibited antifungal activity against M. audouinii, T. rubrum, and C. albicans, while no activity was found against N. gypsea and T. interdigitale. The highest activity was observed on M. audouinii for the aqueous leaf (minimum inhibitory concentration [MIC] of 8 µg/mL) and stem extracts (MIC = 31 µg/mL) and the methanolic leaf extract (MIC = 8 µg/mL). Additionally, some marked morphological alterations were observed on M. audouinii hyphae with all aqueous extracts. Concerning antiage bioassays, the major activity was found against hyaluronidase for the aqueous and methanolic extracts from leaves and the methanolic extract from flowers. Analysis of this last extract by high-performance liquid chromatography, electrospray ionization-mass spectrometry, and nuclear magnetic resonance, allowed us to identify daphnoretin and daphnetin as major components of this extract, and, therefore, responsible for the antiaging activity. In conclusion, this study provides additional experimental data supporting the traditional use of T. hirsuta extracts on some cutaneous dermatophytosis, as well as new perspectives on the potential use of T. hirsuta to ensure better healing of the skin.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Antifungal and Antiaging Evaluation of Aerial Part Extracts ofThymelaea hirsuta(L.) Endl

Amari

Razafimandimby²,

Auberon

et al. 2021

Natural Product Communications

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“…The antibacterial activity of the extracts was evaluated using the agar diffusion test according to the method described by Djermane et al (2020). Mueller-Hinton agar was previously poured into 9 mm Petri dishes and allowed to cool.…”

Section: Agar Disc Diffusion Methodsmentioning

confidence: 99%

Phytochemical profile, in vitro antioxidant and antibacterial activities of different extracts of an Algerian endemic: Teucrium atratum

Sadou,

Gali,

Seridi

et al. 2024

Natural Product Research

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Teucrium atratum Pomel. is a species belonging to the Lamiaceae family used in Algerian folk medicine. The present work essentially aimed to assess the phenolic composition and to evaluate some of the biological effects of different extracts, never previously studied, of T. atratum growing in Algeria. High levels of total phenolic and flavonoids were recorded in the hydromethanolic extract. Chlorogenic acid, isoquercetin, coumarin, cinnamic acid, quercetin dihydrate, and catechin were identified in the methanolic extract by mean of HPLC. The antioxidant activity assessed showed that the methanolic extract was the most active, while, the hydromethanolic extract showed a great power to reduce iron. In addition, all extracts had a significant antibacterial effect against the four tested bacterial strains, with Staphylococcus aureus as the most sensitive one. These findings can be a starting point to evaluate the plant as a source of natural bioactive compounds with antioxidant and antibacterial effects.

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“…DPPH ● radical scavenging according to the method described in the literature [ 68 ], was performed to evaluate the antioxidant potential of different concentrations of investigated extracts. A total of 40 µL of the tested solution (extract or standard) in methanol was added to 160 µL of DPPH ● solution (0.06 mg dissolved in one mL of methanol and having an absorbance of 0.5 to 517 nm).…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition, Anti-Quorum Sensing, Enzyme Inhibitory, and Antioxidant Properties of Phenolic Extracts of Clinopodium nepeta L. Kuntze

Hatem

Boudiba

Benahmed

et al. 2021

Plants

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Phenolic extracts of Clinopodium nepeta were prepared and their preliminary phenolic profiles determined using HPLC-DAD with 26 phenolic standards. Apigenin (21.75 ± 0.41 µg/g), myricetin (72.58 ± 0.57 µg/g), and rosmarinic acid (88.51 ± 0.55 µg/g) were the most abundant compounds in DCM (dichloromethane), AcOEt (ethyl acetate), and BuOH (butanol) extracts, respectively. The DCM and AcOEt extracts inhibited quorum-sensing mediated violacein production by C. violaceum CV12472. Anti-quorum-sensing zones on C. violaceum CV026 at MIC (minimal inhibitory concentration) were 10.3 ± 0.8 mm for DCM extract and 12.0 ± 0.5 mm for AcOEt extract. Extracts showed concentration-dependent inhibition of swarming motility on flagellated P. aeruginosa PA01 and at the highest test concentration of 100 μg/mL, AcOEt (35.42 ± 1.00%) extract displayed the best activity. FRAP assay indicated that the BuOH extract (A0.50 = 17.42 ± 0.25 µg/mL) was more active than standard α-tocopherol (A0.50 = 34.93 ± 2.38 µg/mL). BuOH extract was more active than other extracts except in the ABTS●+, where the DCM extract was most active. This antioxidant activity could be attributed to the phenolic compounds detected. C. nepeta extracts showed moderate inhibition on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, and α-amylase. The results indicate that C. nepeta is a potent source of natural antioxidants that could be used in managing microbial resistance and Alzheimer′s disease.

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Chemical composition and in vitro evaluation of antioxidant, antimicrobial, and enzyme inhibitory activities of Erucaria uncata and Thymeleae hirsuta

Cited by 8 publications

References 51 publications

Antifungal and Antiaging Evaluation of Aerial Part Extracts ofThymelaea hirsuta(L.) Endl

Antifungal and Antiaging Evaluation of Aerial Part Extracts ofThymelaea hirsuta(L.) Endl

Phytochemical profile, in vitro antioxidant and antibacterial activities of different extracts of an Algerian endemic: Teucrium atratum

Chemical Composition, Anti-Quorum Sensing, Enzyme Inhibitory, and Antioxidant Properties of Phenolic Extracts of Clinopodium nepeta L. Kuntze

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