Chemical composition and in vitro activity of Calotropis procera (Ait.) latex on Haemonchus contortus

Cavalcante, Géssica Soares; Morais, Selene Maia de; André, Weibson Paz Pinheiro; Ribeiro, Wesley Lyeverton Correia; Rodrigues, Ana Livya Moreira; Lira, Fábia Cristiane Melo Leite de; Viana, Janaína M.; Beviláqua, Cláudia Maria Leal

doi:10.1016/j.vetpar.2016.06.012

Cited by 32 publications

(17 citation statements)

References 20 publications

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“…AWM is an in vitro test that evaluates the action of compounds isolated from natural products and that can be used to assess the interaction of bioactive compounds with the cuticle of adult nematodes in a short time by SEM (ANDRE et al, 2016; CAVALCANTE et al, 2016). Thymol showed superior action to TA in inhibiting motility of the adult worms.…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic effect of thymol and thymol acetate on sheep gastrointestinal nematodes and their toxicity in mice

André

Cavalcante

Ribeiro

et al. 2017

Rev. Bras. Parasitol. Vet.

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Thymol is a monoterpene and acetylation form of this compound can reduce the toxicity and enhance its biological effects. The objective of this study was to evaluate the effect of thymol and thymol acetate (TA) on egg, larva and adult Haemonchus contortus and the cuticular changes, acute toxicity in mice and the efficacy on sheep gastrointestinal nematodes. In vitro tests results were analyzed by analysis of variance (ANOVA) and followed by comparison with Tukey test or Bonferroni. The efficacy of in vivo test was calculated by the BootStreet program. In the egg hatch test (EHT), thymol (0.5 mg/mL) and TA (4 mg/mL) inhibited larval hatching by 98% and 67.1%, respectively. Thymol and TA (8 mg/mL) inhibited 100% of larval development. Thymol and TA (800 µg/mL) reduced the motility of adult worms, by 100% and 83.4%, respectively. Thymol caused cuticular changes in adult worm teguments. In the acute toxicity test, the LD 50 of thymol and TA were 1,350.9 mg/kg and 4,144.4 mg/kg, respectively. Thymol and TA reduced sheep egg count per gram of faeces (epg) by 59.8% and 76.2%, respectively. In in vitro tests thymol presented better anthelmintic activity than TA. However TA was less toxic and in in vivo test efficacy was similar.Keywords: Acetylation, thymol, Haemonchus contortus, nematodes, sheep. ResumoTimol é um monoterpeno e a acetilação deste composto pode reduzir a toxicidade e potencializar os seus efeitos biológicos. O objetivo deste trabalho foi avaliar o efeito do timol e acetato de timolila (AT) sobre ovos, larvas e adultos de Haemonchus contortus e suas alterações cuticulares, toxicidade aguda em camundongos e a eficácia sobre nematoides gastrintestinais de ovinos. Os resultados dos testes in vitro foram analisados por análise de variância (ANOVA) e comparados pelo testes de Tukey ou Bonferroni. A eficácia do teste de redução da contagem de ovos nas fezes (RCOF) foi calculada pelo programa BootStreet. No teste de inibição da eclosão de ovos (TEO), timol (0,5 mg/mL) e AT (4 mg/mL) inibiram a eclosão das larvas em 98% e 67,1%, respectivamente. Timol e AT (8 mg/mL) inibiram 100% do desenvolvimento larval. Timol e AT (800 µg/mL) reduziram a motilidade dos nematoides adultos, em 100% e 83,4%, respectivamente. O timol provocou alterações cuticulares nos nematoides adultos. No teste de toxicidade aguda, a DL50 do timol e AT foi de 1.350,9 mg/kg e 4.144,4 mg/kg, respectivamente. Timol e AT reduziram a contagem de ovos por gramas de fezes (OPG) dos ovinos em 59,8% e 76,2%, respectivamente. Nos testes in vitro timol apresentou atividade melhor anti-helmíntica do que AT. Entretanto, AT foi menos tóxico do que o timol e no teste in vivo apresentaram eficácia semelhante.

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Section: Discussionmentioning

confidence: 99%

Anthelmintic effect of thymol and thymol acetate on sheep gastrointestinal nematodes and their toxicity in mice

André

Cavalcante

Ribeiro

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…The latex extract inhibited the larvae growth and development by 99.8% at 1 mg/ ml concentration. The extract concentrations also exhibited a dose-dependent manner, with an efficient concentration (EC50) of 1.6 mg/ml and 0.22 mg/ml for EHT and LDT respectively 9 . In the adult Worms Motility Test (WMT), the plant latex extract inhibited the motility of the worms by 100% at 100 mg/ml concentration 9 .…”

Section: Calotropis Proceramentioning

confidence: 94%

“…The extract concentrations also exhibited a dose-dependent manner, with an efficient concentration (EC50) of 1.6 mg/ml and 0.22 mg/ml for EHT and LDT respectively 9 . In the adult Worms Motility Test (WMT), the plant latex extract inhibited the motility of the worms by 100% at 100 mg/ml concentration 9 . Different studies examined the antifungal activity of the proteins presented in the latex of Calotropis procera.…”

Section: Calotropis Proceramentioning

confidence: 94%

“…However, it showed a moderate activity against Epidermophyton floccosum and Microsporum canis fungal strains 5 . A recent work, studied the in vitro effect of the Ethyl Acetate Extract Of Calotropis procera Latex (EAECPL) against one of the common parasites namely Haemonchus contortus 9 . In the Egg Hatching Test (EHT), results showed that the ethyl acetate extract of the plant latex inhibited the larval hatching by 91.8% at a concentration of 4 mg/ml.…”

Section: Calotropis Proceramentioning

confidence: 99%

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Ethnomedicine, phytochemistry and pharmacology of Calotropis procera and Tribulus terrestris

Dirir

Cheruth²,

Ksiksi³

2017

Journal of Natural Remedies

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“…Also, the latex of C. procera has been shown to have antihelmintic effect in sheep [191,192]. Ethyl acetate solutions of lyophilized C. procera latex inhibited egg hatching of H. contortas, showed a pronounced larvicidal effect (EC 50 = 0.22 mg/mL) and inhibited the motility of the parasites by over 80% after 6h contact time [193].…”

Section: Nematicidal/schistosomicidal/antihelminthic Activitymentioning

confidence: 99%

Chemical Constituents and Uses of Calotropis Procera and Calotropis Gigantea – A Review (Part I – The Plants as Material and Energy Resources)

Sulaibi¹,

Thiemann²,

Thiemann³

2020

CHEM

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Chemical composition and in vitro activity of Calotropis procera (Ait.) latex on Haemonchus contortus

Cited by 32 publications

References 20 publications

Anthelmintic effect of thymol and thymol acetate on sheep gastrointestinal nematodes and their toxicity in mice

Anthelmintic effect of thymol and thymol acetate on sheep gastrointestinal nematodes and their toxicity in mice

Ethnomedicine, phytochemistry and pharmacology of Calotropis procera and Tribulus terrestris

Chemical Constituents and Uses of Calotropis Procera and Calotropis Gigantea – A Review (Part I – The Plants as Material and Energy Resources)

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